Murine model of cutaneous infection with gram-positive cocci

Abstract

Staphylococcus aureus has remained an important cause of nosocomial wound infections, but standardized or reproducible systems for analyzing cutaneous infections caused by S. aureus do not exist. A variety of foreign materials, variable inocula, and skin traumas have been used to promote infection. To minimize these variables and ensure reproducibility, we chose a model using subcutaneous injections of a fixed quantity of dextran microbeads (Cytodex) as the foreign material added to standardized broth suspensions of S. aureus.

Intravital two-photon microscopy of host-pathogen interactions in a mouse model ofStaphylococcus aureusskin abscess formation

“…S2). This represents an inoculum, which is orders of magnitude smaller than the inocula conventionally used in S. aureus infection models (Bunce et al, 1992;Tarkowski et al, 2001;Wright et al, 2005).…”

Invertebrates as animal models forStaphylococcus aureuspathogenesis: a window into host–pathogen interaction

“…Since the early 1960s rabbits, rats, and mice have been used to determine various aspects of the pathophysiological characteristics, immunological responses and efficiency of therapeutics during staphylococcal infections in various organs and systems. Although rats remain the preferred model of staphylococcal endocarditis [4] and rabbits for Toxic Shock Syndrome (TSS) [5] mouse models have been developed for a variety of the pathophysiologies generated by S. aureus, including: wound infection [6], arthritis and sepsis [7], mastitis [8], kidney infection [9], and nasal colonization [10]. Consequently, the murine model has been a hallmark contributor towards discerning the breadth of virulence effectors and regulators identified in staphylococcal species.…”

A porcine bimicrobial abscess model for testing interventional treatments

“…It is possible that the injection of a large volume of the bimicrobial mixture would be enough trauma to elicit a robust and sustained inflammatory response and the subsequent generation of an abscess. However, this was not evaluated since many studies have reported the need for an irritant [14,15,28,29]. It is also possible that preinjuring the tissue before inoculation might also eliminate the need for the dextran; this will be evaluated in future studies.…”