Murine monoclonal antibodies to Klebsiella pneumoniae protect against lethal endotoxemia and experimental infection with capsulated K. pneumoniae

Mandine, Eliane; Salles, M.F.; Zalisz, R.; Guenounou, Moncef; Smets, P.

doi:10.1128/iai.58.9.2828-2833.1990

Cited by 25 publications

(11 citation statements)

References 29 publications

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“…During the last decade, numerous murine and human MAbs that recognize common core structures within the LPSs of gram-negative bacteria have been developed (1,5,19,33,38,44,47). Some of these MAbs have been shown to react with individual Klebsiella strains that were not characterized with respect to their 0 group (5,38,44).…”

Section: Discussionmentioning

confidence: 99%

A murine monoclonal antibody defines a unique epitope shared by Klebsiella lipopolysaccharides

et al. 1994

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A hybridoma secreting a monoclonal antibody (MAb) directed against Klebsiella lipopolysaccharide (LPS) was derived from spleen cells of mice immunized with a smooth, nonencapsulated Klebsiella strain (Friedlander 201; serogroup 01). The MAb, called V/9-5 (immunoglobulin G2a), cross-reacted with LPS preparations produced from reference strains for the Klebsiella 0 serogroups 01, 02ab, 02ac, 03, 04, 05, and 012. Furthermore, the MAb reacted with LPSs from serogroup reference strains 06/08, 09, and 011, which are regarded as being identical to 01, 02, and 04, respectively. When testing the supernatant of clinically isolated Kkbsiella strains by means of an inhibition enzyme-linked immunosorbent assay, we found that 86 (92.4%) of 93 Klebsiella pneumoniae subsp. pneumoniae isolates and 24 (96.0%) of 25 K. oxytoca isolates harbored the cross-reactive epitope. By contrast, two laboratory strains of K. pneumoniae subsp. rhinoscleromatis did not react with MAb V/9-5. The MAb proved to be specific for the genus Klebsiella, since it did not react with any of a total of 73 strains belonging to other gram-negative bacterial genera. In conjunction with other LPS-specific MAbs, MAb V/9-5 might become a useful reagent for rapid identification of klebsiellae in clinical specimens. Furthermore, the epitope recognized by MAb V/9-5 might serve as a target epitope for the production of human MAbs for immunotherapeutic purposes.

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Section: Discussionmentioning

confidence: 99%

A murine monoclonal antibody defines a unique epitope shared by Klebsiella lipopolysaccharides

et al. 1994

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“…A multivalent LPS vaccine composed of these eight O antigens or at least the inclusion of the common O1 antigen in a broad spectrum capsular polysaccharide vaccine might be a promising approach. Recently, the administration of monoclonal antibodies to the Klebsiella O1 antigen has been reported to be protective in a mouse model of lethal endotoxemia (136). Moreover, the inclusion of O antigens in a multivalent Klebsiella vaccine formulation might be of additional benefit due to strong adjuvant action, as has been demonstrated for the Klebsiella O3 lipopolysaccharide (259).…”

Section: Lipopolysaccharidesmentioning

confidence: 99%

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

1998

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SUMMARY Bacteria belonging to the genus Klebsiella frequently cause human nosocomial infections. In particular, the medically most important Klebsiella species, Klebsiella pneumoniae, accounts for a significant proportion of hospital-acquired urinary tract infections, pneumonia, septicemias, and soft tissue infections. The principal pathogenic reservoirs for transmission of Klebsiella are the gastrointestinal tract and the hands of hospital personnel. Because of their ability to spread rapidly in the hospital environment, these bacteria tend to cause nosocomial outbreaks. Hospital outbreaks of multidrug-resistant Klebsiella spp., especially those in neonatal wards, are often caused by new types of strains, the so-called extended-spectrum-β-lactamase (ESBL) producers. The incidence of ESBL-producing strains among clinical Klebsiella isolates has been steadily increasing over the past years. The resulting limitations on the therapeutic options demand new measures for the management of Klebsiella hospital infections. While the different typing methods are useful epidemiological tools for infection control, recent findings about Klebsiella virulence factors have provided new insights into the pathogenic strategies of these bacteria. Klebsiella pathogenicity factors such as capsules or lipopolysaccharides are presently considered to be promising candidates for vaccination efforts that may serve as immunological infection control measures.

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“…Klebsiellae are the second most common microorganisms isolated, after E. coli, from gram-negative bacteremia patients (11,17). Al-though the structures of the nine known 0 antigens (13,16,27) have been elucidated, very little research has been done on the core and immunochemistry of Klebsiella LPS and very few MAbs against its core and lipid A have been reported (20).…”

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confidence: 99%

“…All assays were performed with 96-well V-shaped polystyrene microtiter plates. Each well (23,25) R. Zalisz (20) N. Kato, Nagoya, Japan (14) N. Kato 26 P. Kosma 9P. Kosma 9" Strains 8238 to 1754/49 are Orskov strains: the remainder are not Orskov strains.…”

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confidence: 99%

Binding studies of a monoclonal antibody specific for 3-deoxy-D-manno-octulosonic acid with a panel of Klebsiella pneumoniae lipopolysaccharides representing all of the O serotypes

et al. 1994

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A monoclonal antibody (MAb) raised against Salmonella minnesota R595 and specific for ot-3-deoxy-D-mannooctulosonic acid (a-Kdo) of the inner core was tested for binding to lipopolysaccharides (LPS) of Klebsiella pneumoniae. The MAb was tested in several assay systems (enzyme-linked immunosorbent assay, passive hemolysis, and inhibition of passive hemolysis) with a large panel (n = 23) of K. pneumoniae LPS representing all nine currently known 0 serotypes. MAb 20 showed reactivity with almost all 0 serotypes of K. pneumoniae LPS, and this reactivity could be inhibited by synthetic Kdo. This suggests an epitope in the cores of these Klebsiella LPS much like that in the inner core of LPS of S. minnesota. Large differences in reactivity between LPS of different strains belonging to the same 0 serotype were observed. After sodium dodecyl sulfatepolyacrylamide gel electrophoresis of LPS followed by immunoblotting, reactivity of MAb 20 was observed only with the fast-moving fraction possibly representing the nonsubstituted core. No binding was seen with the high-molecular-weight fraction that contained core material substituted with several units of 0-antigen building blocks. The chemical basis for these differences in reactivity remains to be established. As far as we know, this is the first report containing comprehensive immunochemical data on the LPS core of K. pneumoniae.

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Murine monoclonal antibodies to Klebsiella pneumoniae protect against lethal endotoxemia and experimental infection with capsulated K. pneumoniae

Cited by 25 publications

References 29 publications

A murine monoclonal antibody defines a unique epitope shared by Klebsiella lipopolysaccharides

A murine monoclonal antibody defines a unique epitope shared by Klebsiella lipopolysaccharides

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

Binding studies of a monoclonal antibody specific for 3-deoxy-D-manno-octulosonic acid with a panel of Klebsiella pneumoniae lipopolysaccharides representing all of the O serotypes

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