Murine nursing thymic epithelial cell lines capable of inducing thymocyte apoptosis express the self-superantigen Mls-1a

Hiramine, Chiharu; Nakagawa, Toshitaka; Hojo, Kenji

doi:10.1016/0008-8749(95)80021-a

Cited by 10 publications

(12 citation statements)

References 46 publications

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“…A human nursing TEC line was isolated and established using methods described previously for isolation of mouse nursing TEC lines (Hiramine et al, 1990(Hiramine et al, , 1995. These nursing TEC expressed desmosomes and cytokeratin, which are specific for epithelial cells but were negative for Mac-1 (data not shown).…”

Section: Resultsmentioning

confidence: 97%

“…A human nursing TEC line was established from that thymus, which was not invaded by lymphoma cells, as described (Hiramine et al, 1990(Hiramine et al, , 1995. The cells were initially maintained in Eagle's MEM-D-valine medium (Life Technologies) supplemented with D-valine (9.2 mg/100 ml; Wako, Osaka, Japan), 10% FBS, and subsequently the nursing TEC were maintained at 37°C in 5% CO 2 in air at 100% humidity in DMEM medium (Life Technologies) supplemented with 10% FBS and antibiotics.…”

Section: Establishment Of Nursing Tec Linementioning

confidence: 99%

“…The TNC, a thymic lymphoepithelial cell complex, consists of one thymic epithelial cell (TEC) and many thymocytes enclosed by epithelial cytoplasmic processes. It has been considered to be a specialized site for proliferation, differentiation, and maturation of immature thymocytes (Wekerle et al, 1980) and, recently, for positive and negative selection (Hiramine et al, 1995). We have previously reported methods for establishing murine nursing TEC lines capable of binding and enveloping viable immature thymocytes to form TNC (Hiramine et al, 1990(Hiramine et al, , 1995.…”

mentioning

confidence: 99%

“…It has been considered to be a specialized site for proliferation, differentiation, and maturation of immature thymocytes (Wekerle et al, 1980) and, recently, for positive and negative selection (Hiramine et al, 1995). We have previously reported methods for establishing murine nursing TEC lines capable of binding and enveloping viable immature thymocytes to form TNC (Hiramine et al, 1990(Hiramine et al, , 1995. These nursing TEC lines can induce apoptosis mainly in small CD4 ϩ CD8 ϩ thymocytes as well as proliferation/ differentiation of immature thymocytes (Hiramine et al, 1990(Hiramine et al, , 1995.…”

mentioning

confidence: 99%

“…We have previously reported methods for establishing murine nursing TEC lines capable of binding and enveloping viable immature thymocytes to form TNC (Hiramine et al, 1990(Hiramine et al, , 1995. These nursing TEC lines can induce apoptosis mainly in small CD4 ϩ CD8 ϩ thymocytes as well as proliferation/ differentiation of immature thymocytes (Hiramine et al, 1990(Hiramine et al, , 1995. Recently we have demonstrated that nursing TEC in vivo exhibit phagocytic activity in the digestion and clearance of apoptotic thymocytes as shown by the presence of phagolysosomes containing acid phosphatase activity (Hiramine et al, 1996).…”

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confidence: 99%

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Human Scavenger Receptor B1 Is Involved in Recognition of Apoptotic Thymocytes by Thymic Nurse Cells

Imachi

Murao

Hiramine

et al. 2000

Laboratory Investigation

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SUMMARY:Recognition and uptake of apoptotic cells by neighboring phagocytes is essential for the clearance of dying cells without accompanying inflammation or tissue damage. In the thymus, many apoptotic cells are generated in the process of negative selection, and both thymic macrophages (professional phagocytes) and nursing thymic epithelial cells (nursing TEC; nonprofessional phagocytes) recognize and ingest them. However the receptors responsible for this recognition and uptake have not been identified. In the present study, we have established a human nursing TEC line and examined the expression of several genes of the scavenger receptor family considered to be potential receptors for apoptotic cells. Human scavenger receptor-B1 (hSR-B1)/CLA-1, previously shown to recognize apoptotic cells, was strongly expressed in nursing TEC, whereas there was little or no expression of the other scavenger receptors tested: scavenger receptor class A, CD36, or CD68. Suppression of hSR-B1/CLA-1 expression using antisense oligonucleotides decreased the binding of apoptotic thymocytes to nursing TEC by more than 40%. These results indicate that hSR-B1/CLA-1 may play a major role in the clearance of apoptotic cells in the thymus, mediating the recognition and ingestion of apoptotic thymocytes by nursing TEC. (Lab Invest 2000, 80:263-270).

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Section: Resultsmentioning

confidence: 97%

Section: Establishment Of Nursing Tec Linementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Human Scavenger Receptor B1 Is Involved in Recognition of Apoptotic Thymocytes by Thymic Nurse Cells

Imachi

Murao

Hiramine

et al. 2000

Laboratory Investigation

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Selective Antigen Presenting Activity of Cortical Thymic Epithelial Cells against CD4+T Cells Associated with both Lack of Costimulatory Molecules and Inefficient Presentation of MHC–Peptide Ligands

Kaneda

Iwabuchi

Kasai

et al. 1997

Cellular Immunology

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Subtypes of thymic epithelial cells defined by neuroendocrine markers

Brelińska

Ostalska

Zabel

2000

Histochem Cell Biol

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The study attempted to define characteristics of thymic epithelial cells within rat thymus based on the expression of neuroendocrine markers. Using an immunohistochemical approach, the following markers were localised: protein gene product 9.5 (PGP 9.5), neuronspecific enolase (NSE) and chromogranin A (ChA). It was shown that cells displaying immunostaining typical for individual markers reside in distinct regions of the thymus and represent subtypes within various populations of thymic epithelial cells. An immunoreactivity for PGP 9.5 was found exclusively in a subtype of cortical epithelial cells, located mostly within the inner zone of the cortex. On the other hand, NSE represented a marker of most epithelial cells located in the medulla. Few such cells which were negative for NSE proved positive for ChA. Among the cells with a strong reaction for NSE some cells also manifested a positive reaction for ChA. While the pattern of neuroendocrine marker distribution may reflect functional properties of thymic epithelial cells which might be different within distinct areas of the thymus, the differential expression of individual markers seems to reflect biological activity of the cells and/or distinct stages of their differentiation.

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Murine nursing thymic epithelial cell lines capable of inducing thymocyte apoptosis express the self-superantigen Mls-1a

Cited by 10 publications

References 46 publications

Human Scavenger Receptor B1 Is Involved in Recognition of Apoptotic Thymocytes by Thymic Nurse Cells

Human Scavenger Receptor B1 Is Involved in Recognition of Apoptotic Thymocytes by Thymic Nurse Cells

Selective Antigen Presenting Activity of Cortical Thymic Epithelial Cells against CD4+T Cells Associated with both Lack of Costimulatory Molecules and Inefficient Presentation of MHC–Peptide Ligands

Subtypes of thymic epithelial cells defined by neuroendocrine markers

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