Murine pulmonary infection with Listeria monocytogenes: differential susceptibility of BALB/c, C57BL/6 and DBA/2 mice

Munder, Antje; Zelmer, Andrea; Schmiedl, Andreas; Dittmar, Kurt E.J.; Rohde, Manfred; Dorsch, Martina; Otto, K; Hedrich, H.-J.; Tümmler, Burkhard; Weiß, Siegfried; Tschernig, Thomas

doi:10.1016/j.micinf.2004.12.021

Cited by 38 publications

(38 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tento 12-week-old female mice of the inbred strain C3H/HeN (Charles River, Sulzfeld, Germany) were inoculated with 30 l of this bacterial suspension containing 7.5 ϫ 10 6 CFU of the different P. aeruginosa PAO1 sublines via view-controlled intratracheal instillation. In the case of 14-day infection experiments, the weight and rectal temperature of the mice were measured daily and their body condition was scored for the parameters vocalization, piloerection, attitude, locomotion, breathing, curiosity, nasal secretion, grooming, and dehydration (29).…”

Section: Methodsmentioning

confidence: 99%

Genome Diversity ofPseudomonas aeruginosaPAO1 Laboratory Strains

et al. 2010

Self Cite

View full text Add to dashboard Cite

Pseudomonas aeruginosa PAO1 is the most commonly used strain for research on this ubiquitous and metabolically versatile opportunistic pathogen. Strain PAO1, a derivative of the original Australian PAO isolate, has been distributed worldwide to laboratories and strain collections. Over decades discordant phenotypes of PAO1 sublines have emerged. Taking the existing PAO1-UW genome sequence (named after the University of Washington, which led the sequencing project) as a blueprint, the genome sequences of reference strains MPAO1 and PAO1-DSM (stored at the German Collection for Microorganisms and Cell Cultures [DSMZ]) were resolved by physical mapping and deep short read sequencing-by-synthesis. MPAO1 has been the source of near-saturation libraries of transposon insertion mutants, and PAO1-DSM is identical in its SpeI-DpnI restriction map with the original isolate. The major genomic differences of MPAO1 and PAO1-DSM in comparison to PAO1-UW are the lack of a large inversion, a duplication of a mobile 12-kb prophage region carrying a distinct integrase and protein phosphatases or kinases, deletions of 3 to 1,006 bp in size, and at least 39 single-nucleotide substitutions, 17 of which affect protein sequences. The PAO1 sublines differed in their ability to cope with nutrient limitation and their virulence in an acute murine airway infection model. Subline PAO1-DSM outnumbered the two other sublines in late stationary growth phase. In conclusion, P. aeruginosa PAO1 shows an ongoing microevolution of genotype and phenotype that jeopardizes the reproducibility of research. High-throughput genome resequencing will resolve more cases and could become a proper quality control for strain collections.The metabolically versatile Pseudomonas aeruginosa is an opportunistic pathogen of plants, animals, and humans and is ubiquitously distributed in soil and aquatic habitats. The common reference strain is P. aeruginosa PAO1, a spontaneous chloramphenicol-resistant mutant of the original PAO strain (earlier called "P. aeruginosa strain 1") that had been isolated in 1954 from a wound in Melbourne, Australia (9, 10). This PAO1 strain from Bruce Holloway's laboratory has become the reference strain for Pseudomonas genetics and functional analyses of the physiology and metabolism of this gammaproteobacterium. A genetic map of its chromosome was generated by exploiting the mechanisms of gene exchange in bacteria, i.e., transduction and conjugation (11). With the advent of pulsedfield gel electrophoresis (PFGE), a physical map of the PAO1 genome was constructed (32) and later merged with the genetic map information (12). By 2000 the PAO1 strain had been completely sequenced (36). Thereafter, the genome annotation has been continually updated and the database content and functionality have been expanded to facilitate accelerated discovery of P. aeruginosa drug targets and vaccine candidates (38). Two near-saturation libraries of transposon insertion mutants have been constructed in P. aeruginosa PAO1 as a global resource for the scient...

show abstract

Section: Methodsmentioning

confidence: 99%

Genome Diversity ofPseudomonas aeruginosaPAO1 Laboratory Strains

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other studies have focused on the variation in longevity and the role of caloric restriction in extending life span (16,17). In the context of the lung, these two strains exhibit differential susceptibilities to a wide variety of stresses, including pulmonary infection (45,50), hypersensitivity pneumonitis (19), ovalbumin-induced pulmonary eosinophilia, and cigarette smoke-induced emphysema (4). In the latter study (4), D2 mice exhibited a greater susceptibility to severe emphysematous changes following cigarette smoke exposure, which was characterized by more uniform and earlier onset of air space enlargement compared with B6 mice.…”

mentioning

confidence: 99%

Global expression profiles from C57BL/6J and DBA/2J mouse lungs to determine aging-related genes

Misra

Lee

Singh

et al. 2007

Physiological Genomics

View full text Add to dashboard Cite

This study identified gene expression profiles that provided evidence for genomic mechanisms underlying the pathophysiology of aging lung. Aging lungs from C57BL/6 (B6) and DBA/2 (D2) mouse strains differ in physiology and morphometry. Lungs were harvested from B6 mice at 2, 18, and 26 mo and from D2 mice at 2 and 18 mo of age. Purified RNA was subjected to oligonucleotide microarray analyses, and differential expression analyses were performed for comparison of various data sets. A significant majority of differentially expressed genes were upregulated with aging in both strains. Aging D2 lungs uniquely exhibited upregulation in stress-response genes including xenobiotic detoxification cascades. In contrast, aging B6 lungs showed downregulation of heat shock-response genes. Age-dependent downregulation of genes common to both B6 and D2 strains included several collagen genes (e.g., Col1a1 and Col3a1). There was a greater elastin gene (Eln) expression in D2 mice at 2 mo, and Eln was uniquely downregulated with age in this strain. The matrix metalloproteinase 14 gene (Mmp14), critical to alveolar structural integrity, was also downregulated with aging in D2 mice only. Several polymorphisms in the regulatory and untranslated regions of Mmp14 were identified between strains, suggesting that variation in Mmp14 gene regulation contributes to accelerated aging of lungs in D2 mice. In summary, lungs of B6 and D2 mice age with variable rates at the gene expression level, and these quantifiable genomic differences provide a template for understanding the variability in age-dependent changes in lung structure and function.Mmp14; lung elasticity; lung senescence; microarray; mouse single nucleotide polymorphisms GLOBAL GENE EXPRESSION PROFILING is a powerful tool to generate hypotheses for less understood processes and has been used to study aging-associated gene expression changes in a variety of mouse tissues (8,9,25,27,35,36). However, differential gene expression analyses have not been applied to the aging lung, a critical primary organ in the defense against environmental insults such as airborne pollutants. To date, only one study has documented global expression in healthy human lungs (18) as a function of age. Although this study did not actually achieve statistical significance in gene expression variation with age, modest global differences in gene expression between young and old subjects were observed.The present study offers certain alternatives relative to studies using clinical samples by employing inbred mouse strains. The use of inbred mice achieves several advantages such as isogenicity and genomewide homozygosity among individuals within a strain, which significantly reduces gene expression variability between individuals. This variability was confounding in the aging human study (18). Aging mouse models have been used, for example, to evaluate global gene expression changes in skeletal muscle (35). One of the primary findings with aged skeletal muscle suggested that stress-response genes, including heat...

show abstract

“…Although both ART2.1 and ART2.2 act as ecto-ARTs, only ART2.1 requires the presence of extracellular thiol cofactors to modulate a reactive cysteine uniquely present at position 201 in ART2.1 (19,20). Interestingly, in the three-dimensional structure of rat ART2.2, the side chains of Ser 60 and Phe 181 , i.e., the residues corresponding to the extra cysteine residues in ART2.1, are in close proximity, suggesting that the two cysteines may form a labile disulfide bridge in ART2.1 (Fig. 11) (49, 50).…”

Section: Discussionmentioning

confidence: 99%

“…LPS and IFN-␥, but not IFN-␤, induced a modest accumulation of (49, 50)). The side chains of residue Ser 60 and Phe 181 are shown as stick models. At the corresponding positions, mouse ART2.1 carries cysteine residues unique in the ART family.…”

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide, IFN-γ, and IFN-β Induce Expression of the Thiol-Sensitive ART2.1 Ecto-ADP-Ribosyltransferase in Murine Macrophages

Hong

Brass²,

Séman

et al. 2007

The Journal of Immunology

View full text Add to dashboard Cite

Extracellular NAD modulates multiple immune and inflammatory responses via the activity of a family of ecto‐ADP‐ribosyltransferases (ARTs) that covalently modify target proteins at arginine or cysteine residues. Functional ARTs have been identified thus far in neutrophils and T cells but not macrophages. This study investigates the expression and function of ARTs by RT‐PCR and western blot analysis in primary bone‐marrow‐derived macrophages (BMDM) isolated from Balb/c mice. Although naive BMDM did not express any of the 6 murine ART genes at the mRNA or protein level, stimulation with lipopolysaccharide (LPS) or ligands for other Toll‐like receptors induced robust expression of ART2.1 as a GPI‐anchored cell surface ecto‐enzyme. ART2.1 expression was potentiated by pharmacological inhibition of ERK1/2 pathways but inhibited by pharmacological blockade of NFkappaB‐ or PI3K‐ signaling pathways. Significantly, catalytic enzyme activity of the inducible ART2.1 was strictly dependent on the presence of extracellular thiol reducing molecules such as cysteine or dithiolthreitol. These data indicate that modulation of immune function by NAD‐dependent ART2.1 activity in macrophages may be synergistically regulated by extracellular redox environments that occur during hypoxia or ishchemia. Support: NIH‐GM‐36387

show abstract

Murine pulmonary infection with Listeria monocytogenes: differential susceptibility of BALB/c, C57BL/6 and DBA/2 mice

Cited by 38 publications

References 35 publications

Genome Diversity ofPseudomonas aeruginosaPAO1 Laboratory Strains

Genome Diversity ofPseudomonas aeruginosaPAO1 Laboratory Strains

Global expression profiles from C57BL/6J and DBA/2J mouse lungs to determine aging-related genes

Lipopolysaccharide, IFN-γ, and IFN-β Induce Expression of the Thiol-Sensitive ART2.1 Ecto-ADP-Ribosyltransferase in Murine Macrophages

Contact Info

Product

Resources

About