Murine T cell-specific Ia antigens: monoclonal antibodies define an I-A-encoded T lymphocyte structure.

Hayes, Colleen E.; Hullett, Debra A.

doi:10.1073/pnas.79.11.3594

Cited by 12 publications

(4 citation statements)

References 39 publications

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“…The above earlier observations led to a logical postulate that the murine I region should accommodate a new multigene family expressed on a series of functional T cells and playing important regulatory roles in H-2-restricted cell interactions , which later turned out to be totally wrong. However, a large number of T cell hybridomas and clones was established, and monoclonal antibodies reactive with I-J and I-AT polypeptides have been produced (Hayes et al 1982, Hiramatsu et al 1981, Kanno et al 1981, Wallenbaugh 1981. Biochemical analysis of the I-J and 1-AT polypeptides has begun (Kumagai et al 1984, Miyatani et al 1983, and the in vitro translation of mRNA from I-Jpositive hybridomas was successful (Taniguchi et al 1982.…”

Section: ) I-j Belongs To a Larger Family Of Molecules (Iat) Determimentioning

confidence: 99%

I‐J as an Inducible T Cell Receptor for Self

Tada

Uracz

Ryo

et al. 1985

Immunological Reviews

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“Jedenfalls, wenn man davorsteht, dann sieht man sich selbstaber eben nicht wie in einem gewöhnlichen Spiegel, versteht sich. Man sieht nicht sein Äusseres, sondern man sieht sein wahres inneres Wesen. so wie es in Wirklichkeit beschaffen ist. Wer da durch will, der muß ‐ um es mal so auszudrücken ‐in sich selbst hineingehen.” Die undendliche Geschichte, bei Michael Ende, K. Thiehemanns Verlag, Stuttgart, 1979.

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Section: ) I-j Belongs To a Larger Family Of Molecules (Iat) Determimentioning

confidence: 99%

I‐J as an Inducible T Cell Receptor for Self

Tada

Uracz

Ryo

et al. 1985

Immunological Reviews

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“…Fused cells were dispensed into 10 96-well plates giving clonal growth in medium with hypoxanthine, aminopterin, and thymidine (12). An enzyme-linked immunosorbent assay (12) or a microcytotoxicity assay (I 3) identified clones secreting I-Jk-specific antibodies. Assays used B10.A(3R) and B10.A(5R) T cells.…”

mentioning

confidence: 99%

“…B10.A(5R)-immune B10.A(3R) splenocytes were fused with P3NS1/1-Ag4-1 (10) or Sp2/0-Agl4 (11) exactly as described (12). Fused cells were dispensed into 10 96-well plates giving clonal growth in medium with hypoxanthine, aminopterin, and thymidine (12). An enzyme-linked immunosorbent assay (12) or a microcytotoxicity assay (I 3) identified clones secreting I-Jk-specific antibodies.…”

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confidence: 99%

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T cell surface I-J glycoprotein. Concerted action of chromosome-4 and -17 genes forms an epitope dependent on alpha-D-mannosyl residues.

Klyczek¹,

Cantor²,

Hayes³

1984

The Journal of Experimental Medicine

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Two genes acting in concert control murine T cell I-Jk expression. We determined I-Jk expression with I-Jk--specific monoclonal antibodies WF8 .C12.8 and five others produced in our laboratory in a cytotoxicity assay. Previous experiments established that an H-2k gene and a chromosome 4 gene, Jt , regulate I-Jk expression. We show here that B10. HTT and B10.S( 9R ) do not differ at the H-2k locus required for I-Jk expression. Rather B10. HTT , like B10.A(3R), lacks some important non--H-2 gene (possibly Jt ). The intra--H-2k I-J--controlling locus maps to the right of the I-A subregion. The I-Jk determinant involves a carbohydrate structure associated with protein; inhibiting either protein synthesis or glycosylation prevents T cell I-Jk reexpression after proteolytic removal. Treatment with alpha-mannosidase destroys I-Jk determinants, implicating terminal alpha-D-mannosyl residues in the I-Jk epitope. Models for H-2 and Jt control of I-J expression are discussed.

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Ir gene expression on T cells: Effect of a monoclonal antibody directed against I region‐controlled determinants on T cells

et al. 1986

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A monoclonal antibody directed at an I region-controlled epitope uniquely expressed on T cells (Iat) was studied for its in vivo effect on the antibody response under the control of an Ir gene. The antibody was produced by a hybridoma made from A.TH spleen cells immune to A.TL (anti-Ik), that was selected for its reactivity with T but not B cells and macrophages, and thus was designated as anti-IatK. The injection of this anti-Iatk into H-2k, H-2b and H-2k X bF1 mice resulted in the suppression of antibody response to poly-L-(His,Glu)-poly-D,L-Ala--poly-L-Lys [(H,G)-A--L] in H-2k and F1 mice but not that to poly-L-(Tyr,Glu)-poly-D,L-Ala--poly-L-Lys [(T,G)-A--L] both in H-2b and F1 mice. The adoptive cell transfer of the combinations of anti-Iatk- or normal mouse serum-treated T and B cells into irradiated hosts demonstrated that anti-Iatk primarily affected (H,G)-A--L-specific helper T cells but not B cells and macrophages, resulting in the specific elimination of the antibody response. Suppressor T cells were not induced by the treatment with anti-Iatk. The antibody specifically eliminated the (H,G)-A--L-specific but not (T,G)-A--L-specific helper T cells in F1 spleen cells that had been primed with both (H,G)-A--L and (T,G)-A--L. The results indicated that anti-Iatk affected the H-2k-associated Ir gene function born by T cells but not by antigen-presenting cells, which was expressed on F1 helper T cells with apparent exclusion of the other allele, and implied that the Iat antigen on helper T cells is one of the sites of expression of Ir genes.

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Murine T cell-specific Ia antigens: monoclonal antibodies define an I-A-encoded T lymphocyte structure.

Cited by 12 publications

References 39 publications

I‐J as an Inducible T Cell Receptor for Self

I‐J as an Inducible T Cell Receptor for Self

T cell surface I-J glycoprotein. Concerted action of chromosome-4 and -17 genes forms an epitope dependent on alpha-D-mannosyl residues.

Ir gene expression on T cells: Effect of a monoclonal antibody directed against I region‐controlled determinants on T cells

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