Musa balbisiana and Musa paradisiaca Starches Increase SCFA and Caspase-3 as well as Decrease β-glucuronidase and MDA of Mouse Model for Colon Cancer

Abstract

BACKGROUND: Administration of resistant starch (RS) influences the diversity and the composition of microbiota as well as inhibits the growth of cancer cell. Banana as a potential source of RS has been reported. Although Musa paradisiaca has been reported to induce apoptosis in colon cancer cells, Musa balbisiana, which has low glycemic index and suitable for particular patients, has not been investigated yet.METHODS: Starches of M. balbisiana and M. paradisiaca were prepared and mixed with other components to make 3 types of mouse pellets. Mouse model for colon cancer was prepared and fed with different types of mouse pellets. Blood was collected and processed for measuring β-glucuronidase and malondialdehyde (MDA) with Enzyme-linked Immunosorbent Assay (ELISA) method. Resected ceca were incised to collect the inner part for short-chain fatty acid (SCFA) measurement with gas chromatography analysis. Resected colas were fixed and processed for immunohistochemistry to detect Caspase-3.RESULTS: Colon-cancer-mice fed with the M. balbisiana and M. paradisiaca starches-contained pellets had significant higher concentrations of total SCFA (p=0.003), acetic acid (p=0.000), propionic acid (p=0.000) and butyric acid (p=0.000); lower concentration of β-glucuronidase (p<0.001); higher Caspase-3 score (p=0.040); and lower MDA concentration (p<0.001) than colon-cancer-mice fed with standard pellet (control).CONCLUSION: M. balbisiana and M. paradisiaca starches could be suggested as potential anti-colon cancer RS. Further research should be carried out to disclose the starches mechanisms in colon cancer cell.KEYWORDS: Musa balbisiana, Musa paradisiaca, colon cancer, resistant starch, Caspase-3, SCFA, β-glucuronidase, malondialdehyde

ANALISIS ZAT GIZI ES KRIM PISANG BATU (Musa balbisiana colla) SEBAGAI PANGAN FUNGSIONAL PENCEGAH KANKER KOLOREKTAL

“…10,11 Pati resisten yang terdapat pada pisang batu juga dapat memperbaiki profil senyawa kimia dalam tubuh yang berhubungan dengan kanker kolon. 12 Kandungan kalsium dan serat pangan yang terdapat pada tepung pisang batu diharapakan dapat menjadi alternatif peningkatan asupan kalsium dan serat pangan melalui pengolahan pisang batu menjadi es krim dengan penambahan tepung pisang batu. Dari hasil penelitian ini diharapkan es krim pisang batu menjadi pangan fungsional pencegah kanker kolorektal.…”

“…Hasil fermentasi yang dihasilkan oleh mikrobiota tersebut salah satunya adalah short chain fatty acid (SCFA) terutama asam asetat, butirat, dan propionate. 11,12 SCFA tersebut memiliki peran penting dalam menjaga kestabilan sel kolon dan memiliki sejumlah fungsi krusial dalam pencegahan kanker kolon. 11 SCFA dapat mempengaruhi proliferasi kolonosit norma dengan konsentrasi yang rendah dan menghambat pertumbuhan sel kanker kolon pada manusia dengan menghentikan siklus sel dan apoptosis melalui regulasi molekuler yang kompleks.…”