Muscarinic receptor control of pyramidal neuron membrane potential in the medial prefrontal cortex (mPFC) in rats

Kurowski, Przemysław; Gawlak, Maciej; Szulczyk, Paweł

doi:10.1016/j.neuroscience.2015.07.023

Cited by 29 publications

(24 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings indicated that the antibodies effectively detected Nav1.9 channels. mPFC pyramidal neurons in our study were immunolabeled with Nav1.9 antibodies, results consistent with those of earlier studies indicating the expression of these channels in the central nervous system (Dib‐Hajj et al, ; Blum et al, ; Blum and Konnerth, ; Subramanian et al, ; Wetzel et al, ; Black et al, ; Kurowski et al, ; Radzicki et al, ).…”

Section: Discussionsupporting

confidence: 91%

“…Whole-Cell Current Recordings from Layer II and V Pyramidal Neurons in Slices. Slices for electrophysiological recordings were prepared as previously described (Kurowski et al, 2015). Prior to the current recordings, the slices were kept for 40 min in a warm (348C) extracellular solution containing the following (in mM): NaCl (130), KCl (2.5), NaH 2 PO 4 (1.25), NaHCO 3 (25), MgCl 2 (2), CaCl 2 (2), and glucose (10).…”

Section: Electrophysiologymentioning

confidence: 99%

“…TTX-Resistant Na 1 Currents in Dispersed Neurons. We sought to obtain electrophysiological evidence that TTX-resistant, low-threshold Nav1.9-like Na 1 currents are present in mPFC pyramidal neurons, as suggested by our earlier study (Kurowski et al, 2015). Therefore, whole-cell membrane currents were recorded from dispersed mPFC pyramidal neurons from young rats.…”

Section: Ttx-resistant Na 6 Currents In Mpfc Pyramidal Neuronsmentioning

confidence: 99%

“…However, other studies have shown that these channels are expressed throughout the central nervous system (Blum et al, ; Blum and Konnerth, ; Subramanian et al, ; Wetzel et al, ; Black et al, ). Recently, it was reported that Nav1.9 channels are expressed in layer V mPFC pyramidal neurons (Kurowski et al, ) and that Nav1.9 transcripts are expressed in layer V mPFC tissue (Radzicki et al, ) of young rats.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Age‐dependent expression of Nav1.9 channels in medial prefrontal cortex pyramidal neurons in rats

Gawlak

Szulczyk

Berłowski

et al. 2017

Developmental Neurobiology

View full text Add to dashboard Cite

Developmental changes that occur in the prefrontal cortex during adolescence alter behavior. These behavioral alterations likely stem from changes in prefrontal cortex neuronal activity, which may depend on the properties and expression of ion channels. Nav1.9 sodium channels conduct a Na current that is TTX resistant with a low threshold and noninactivating over time. The purpose of this study was to assess the presence of Nav1.9 channels in medial prefrontal cortex (mPFC) layer II and V pyramidal neurons in young (20-day old), late adolescent (60-day old), and adult (6- to 7-month old) rats. First, we demonstrated that layer II and V mPFC pyramidal neurons in slices obtained from young rats exhibited a TTX-resistant, low-threshold, noninactivating, and voltage-dependent Na current. The mRNA expression of the SCN11a gene (which encodes the Nav1.9 channel) in mPFC tissue was significantly higher in young rats than in late adolescent and adult rats. Nav1.9 protein was immunofluorescently labeled in mPFC cells in slices and analyzed via confocal microscopy. Nav1.9 immunolabeling was present in layer II and V mPFC pyramidal neurons and was more prominent in the neurons of young rats than in the neurons of late adolescent and adult rats. We conclude that Nav1.9 channels are expressed in layer II and V mPFC pyramidal neurons and that Nav1.9 protein expression in the mPFC pyramidal neurons of late adolescent and adult rats is lower than that in the neurons of young rats. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1371-1384, 2017.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Electrophysiologymentioning

confidence: 99%

Section: Ttx-resistant Na 6 Currents In Mpfc Pyramidal Neuronsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Age‐dependent expression of Nav1.9 channels in medial prefrontal cortex pyramidal neurons in rats

Gawlak

Szulczyk

Berłowski

et al. 2017

Developmental Neurobiology

View full text Add to dashboard Cite

show abstract

“…; Kurowski et al . ). Some of these effectors, including transient receptor potential cation (TRPC) (Yan et al .…”

Section: Discussionmentioning

confidence: 97%

A unifying hypothesis for M1 muscarinic receptor signalling in pyramidal neurons

Dasari

Hill

Gulledge

2016

The Journal of Physiology

View full text Add to dashboard Cite

G -coupled M1-type muscarinic acetylcholine (ACh) receptors (mAChRs) mediate two distinct electrophysiological responses in cortical pyramidal neurons: transient inhibition driven by calcium-dependent small conductance potassium ('SK') channels, and longer-lasting and voltage-dependent excitation involving non-specific cation channels. Here we examine the interaction of these two cholinergic responses with respect to their contributions to intracellular calcium dynamics, testing the 'unifying hypothesis' that rundown of inhibitory SK responses at resting membrane potentials (RMPs) reflects depletion of intracellular calcium stores, while mAChR-driven excitation acts to refill those stores by promoting voltage-dependent entry of extracellular calcium. We report that fidelity of cholinergic SK responses requires the continued presence of extracellular calcium. Inhibitory responses that diminished after repetitive ACh application at RMPs were immediately rescued by pairing mAChR stimulation with subthreshold depolarization (∼10 mV from RMPs) initiated with variable delay (up to 500 ms) after ACh application, but not by subthreshold depolarization preceding mAChR stimulation. Further, rescued SK responses were time-locked to ACh application, rather than to the timing of subsequent depolarizing steps, suggesting that cholinergic signal transduction itself is not voltage-sensitive, but that depolarization facilitates rapid cycling of extracellular calcium through the endoplasmic reticulum to activate SK channels. Consistent with this prediction, rescue of SK responses by subthreshold depolarization required the presence of extracellular calcium. Our results demonstrate that, in addition to gating calcium release from intracellular stores, mAChR activation facilitates voltage-dependent refilling of calcium stores, thereby maintaining the ongoing fidelity of SK-mediated inhibition in response to phasic release of ACh.

show abstract

Valproic acid inhibits TTX-resistant sodium currents in prefrontal cortex pyramidal neurons

Szulczyk

Nurowska

2017

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Muscarinic receptor control of pyramidal neuron membrane potential in the medial prefrontal cortex (mPFC) in rats

Cited by 29 publications

References 91 publications

Age‐dependent expression of Nav1.9 channels in medial prefrontal cortex pyramidal neurons in rats

Age‐dependent expression of Nav1.9 channels in medial prefrontal cortex pyramidal neurons in rats

A unifying hypothesis for M1 muscarinic receptor signalling in pyramidal neurons

Valproic acid inhibits TTX-resistant sodium currents in prefrontal cortex pyramidal neurons

Contact Info

Product

Resources

About