Muscarinic receptors in the nucleus accumbens shell play different roles in context-induced or morphine-challenged expression of behavioral sensitization in rats

Liu, Xinhe; Tian, Lin; Cui, Ruisi; Ruan, Heng; Li, Xinwang

doi:10.1016/j.ejphar.2017.11.039

Cited by 6 publications

(2 citation statements)

References 46 publications

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“…M1, M3, and M5 are enriched in pyramidal neurons (Vilaró et al, 1990 ; Levey et al, 1995 ) and lead to Ca 2+ influx and activate intracellular signaling cascades by coupling to Gq proteins (Wess, 2003 ), while M2 and M4 are expressed mostly in nonpyramidal neurons (Levey et al, 1995 ) and coupled to G i/o proteins to inhibit hippocampal inhibitory interneurons (Wess, 2003 ). Therefore, a mild concentration of mAChR (10 μg/2 μl of scopolamine) blocker was used to evaluate mAChR function in MS–dCA1 circuits (Liu et al, 2018 ; Figure 6A ). Based on the decreased cholinergic fEPSP amplitude shown in Figure 4 , and the previously reported disrupted central muscarinic system in AD (Scarr, 2012 ), we conjectured that the application of muscarinic inhibitor would lead to a bigger drop of the MS–dCA1 fEPSP in 2VO rats.…”

Section: Resultsmentioning

confidence: 99%

Cholinergic Dysfunction Involvement in Chronic Cerebral Hypoperfusion-Induced Impairment of Medial Septum–dCA1 Neurocircuit in Rats

Zhang

Sun

et al. 2020

Front. Cell. Neurosci.

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Chronic cerebral hypoperfusion (CCH) is considered a preclinical condition of mild cognitive impairment and thought to precede dementia. However, as the principal cholinergic source of hippocampus, whether the septo-hippocampal neurocircuit was impaired after CCH is still unknown. In this study, we established the CCH rat model by bilateral common carotid artery occlusion (2VO). Under anesthesia, the medial septum (MS) of rats was stimulated to evoke the field excitatory post-synaptic potential (fEPSP) in the pyramidal cell layer of dCA1. Consequently, we observed decreased amplitude of fEPSP and increased paired-pulse ratio (PPR) after 8-week CCH. After tail pinch, we also found decreased peak frequency and shortened duration of hippocampal theta rhythm in 2VO rats, indicating the dysfunction of septo-hippocampal neurocircuit. Besides, by intracerebroventricularly injecting GABAergic inhibitor (bicuculline) and cholinergic inhibitors (scopolamine and mecamylamine), we found that CCH impaired both the pre-synaptic cholinergic release and the post-synaptic nAChR function in MS–dCA1 circuits. These results gave an insight into the role of CCH in the impairment of cholinergic MS–dCA1 neurocircuits. These findings may provide a new idea about the CCH-induced neurodegenerative changes.

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Section: Resultsmentioning

confidence: 99%

Cholinergic Dysfunction Involvement in Chronic Cerebral Hypoperfusion-Induced Impairment of Medial Septum–dCA1 Neurocircuit in Rats

Zhang

Sun

et al. 2020

Front. Cell. Neurosci.

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“…Rada and colleagues 26 induced morphine withdrawal in rats and discovered that there was a decline in dopamine and a surge in acetylcholine concentration in the nucleus accumbens. Acetylcholine elevation is responsible for the increase in peristaltic movement, resulting in the diarrhea and abdominal contraction associated with withdrawal syndrome 27 . Many studies have also proven that withdrawal signs are due to the locus coeruleus 28,29 .…”

Section: Discussionmentioning

confidence: 99%

Xylopic acid from Xylopia aethiopica (Annonaceae) inhibits morphine tolerance in rats

Mante¹,

Abakah-Ewusi²,

Mingle³

et al. 2022

Pharm Sci Asia

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Traditionally, Xylopia aethiopica is used to manage pain disorders such as neuralgia, colic pain, rheumatism and headache. Using animal models, this study aimed to investigate the ability of Xylopic Acid (XA), a kaurene diterpene obtained from Xylopia aethiopica, to cause tolerance when administered alone or combined with morphine. Development of withdrawal symptoms on discontinuation was also investigated. Tolerance to morphine was induced in rats through an 8-day regimen of chronic administration of morphine (10 mg/kg; twice daily). Effects of XA alone (100 mg/kg) or XA (10-100 mg/kg) on morphine tolerance and withdrawal syndrome precipitated with naloxone hydrochloride (3 mg/kg) were also assessed. XA's mechanism of action was then explored through drug-receptor binding.Chronic morphine administration in rats resulted in analgesic tolerance and morphine withdrawal syndrome. Chronic XA administration did not result in tolerance to XA's antinociceptive effect. Development of morphine withdrawal syndrome precipitated by naloxone and morphine tolerance was significantly (F(12, 60)=29.88, p<0.0001) inhibited by XA. Xylopic acid inhibited development of diarrhea, jumps and weight loss. Pretreatment with α-2-adrenoceptor antagonist, yohimbine, 5HT3 antagonist, ondansetron and muscarinic antagonist, atropine, significantly (p=0.0042) blocked the inhibitory effect of XA on withdrawal jumps. Pretreatment with naloxone produced similar effects on withdrawal jumps as XA alone. Drug-receptor binding assays revealed a lack of significant interaction of XA on alpha-2 adrenoceptors (A, B, C) but exhibited significant DOR-selective antagonism similar to naltrindole. This study reveals that xylopic acid significantly inhibits morphine antinociceptive tolerance and withdrawal in rats. This is the first report of xylopic acid's antagonism on delta opioidergic receptors and potential as an inhibitor of chronic morphine tolerance.

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5-HT2A receptor- and M1 muscarinic acetylcholine receptor-mediated activation of Gαq/11 in postmortem dorsolateral prefrontal cortex of opiate addicts

et al. 2021

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Muscarinic receptors in the nucleus accumbens shell play different roles in context-induced or morphine-challenged expression of behavioral sensitization in rats

Cited by 6 publications

References 46 publications

Cholinergic Dysfunction Involvement in Chronic Cerebral Hypoperfusion-Induced Impairment of Medial Septum–dCA1 Neurocircuit in Rats

Cholinergic Dysfunction Involvement in Chronic Cerebral Hypoperfusion-Induced Impairment of Medial Septum–dCA1 Neurocircuit in Rats

Xylopic acid from Xylopia aethiopica (Annonaceae) inhibits morphine tolerance in rats

5-HT2A receptor- and M1 muscarinic acetylcholine receptor-mediated activation of Gαq/11 in postmortem dorsolateral prefrontal cortex of opiate addicts

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