Muscle Changes in Aging

Siparsky, Patrick N.; Kirkendall, Donald T.; Garrett, William E.

doi:10.1177/1941738113502296

Cited by 139 publications

(64 citation statements)

References 40 publications

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“…Nucleation was also affected by the cytokine, since the number of nuclei per visible fiber was reduced from 9 to 5 for the concentration of 80 ng/mL ( Figure 3C). All these morphological changes were consistent with those related to sarcopenia induced by TNF-α 25,26,30,39,40 . In order to test whether functional changes were also compatible, we set the concentration of TNF-α to 40 ng/mL for the remaining experiments, since it was the concentration for which some effects could be observed without becoming too adverse.…”

Section: Resultssupporting

confidence: 85%

3D-printed drug testing platform based on a 3D model of aged human skeletal muscle

Mestre

Garcia

Patiño

et al. 2020

Preprint

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Three-dimensional engineering of skeletal muscle is becoming increasingly relevant for tissue engineering, disease modeling and bio-hybrid robotics, where flexible, versatile and multidisciplinary approaches for the evaluation of tissue differentiation, functionality and force measurement are required. This works presents a 3D-printed platform of bioengineered human skeletal muscle which can efficiently model the three-dimensional structure of native tissue, while providing information about force generation and contraction profiles. Proper differentiation and maturation of myocytes is demonstrated by the expression of key myo-proteins using immunocytochemistry and analyzed by confocal microscopy, and the functionality assessed via electrical stimulation and analysis of contraction kinetics. To validate the flexibility of this platform for complex tissue modelling, the bioengineered muscle is treated with tumor necrosis factor α to mimic the conditions of aged or senescence-like tissue, which is supported by morphological and functional changes. Moreover, as a proof of concept, the effects of Argireline ® Amplified peptide, a cosmetic ingredient that causes muscle relaxation, are evaluated in both healthy and aged tissue models. Therefore, the results demonstrate that this 3D-bioengineered human muscle platform could be used to assess morphological and functional changes in the aging process of muscular tissue with potential applications in biomedicine, cosmetics and bio-hybrid robotics.

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Section: Resultssupporting

confidence: 85%

3D-printed drug testing platform based on a 3D model of aged human skeletal muscle

Mestre

Garcia

Patiño

et al. 2020

Preprint

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“…84 With the aging process, other contributing factors include state of chronic inflammation, 85 functional and structural decline of the neuromuscular systems, lower muscle turnover and repair capacity due to decreased muscle protein synthesis, and altered endocrine function. [86][87][88][89][90] Our study showed that the positive association between sarcopenia with falls and fractures was independent of most of the applied sarcopenia definitions. However, using the EWGSOP and IWGS definitions, which include low physical performance and/or grip strength in addition to low muscle mass in their diagnostic algorithm, 24 higher risks of falls and fractures among sarcopenic individuals compared with nonsarcopenic individuals were shown.…”

Section: Discussionmentioning

confidence: 60%

Sarcopenia and its association with falls and fractures in older adults: A systematic review and meta‐analysis

Yeung

Reijnierse

Pham

et al. 2019

J cachexia sarcopenia muscle

650

463

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Sarcopenia is a potentially modifiable risk factor for falls and fractures in older adults, but the strength of the association between sarcopenia, falls, and fractures is unclear. This study aims to systematically assess the literature and perform a meta‐analysis of the association between sarcopenia with falls and fractures among older adults. A literature search was performed using MEDLINE, EMBASE, Cochrane, and CINAHL from inception to May 2018. Inclusion criteria were the following: published in English, mean/median age ≥ 65 years, sarcopenia diagnosis (based on definitions used by the original studies' authors), falls and/or fractures outcomes, and any study population. Pooled analyses were conducted of the associations of sarcopenia with falls and fractures, expressed in odds ratios (OR) and 95% confidence intervals (CIs). Subgroup analyses were performed by study design, population, sex, sarcopenia definition, continent, and study quality. Heterogeneity was assessed using the I 2 statistics. The search identified 2771 studies. Thirty‐six studies (52 838 individuals, 48.8% females, and mean age of the study populations ranging from 65.0 to 86.7 years) were included in the systematic review. Four studies reported on both falls and fractures. Ten out of 22 studies reported a significantly higher risk of falls in sarcopenic compared with non‐sarcopenic individuals; 11 out of 19 studies showed a significant positive association with fractures. Thirty‐three studies (45 926 individuals) were included in the meta‐analysis. Sarcopenic individuals had a significant higher risk of falls (cross‐sectional studies: OR 1.60; 95% CI 1.37–1.86, P < 0.001, I 2 = 34%; prospective studies: OR 1.89; 95% CI 1.33–2.68, P < 0.001, I 2 = 37%) and fractures (cross‐sectional studies: OR 1.84; 95% CI 1.30–2.62, P = 0.001, I 2 = 91%; prospective studies: OR 1.71; 95% CI 1.44–2.03, P = 0.011, I 2 = 0%) compared with non‐sarcopenic individuals. This was independent of study design, population, sex, sarcopenia definition, continent, and study quality. The positive association between sarcopenia with falls and fractures in older adults strengthens the need to invest in sarcopenia prevention and interventions to evaluate its effect on falls and fractures.

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“…With aging, various physiological changes occur, although muscle aging is prominent 1 . Age-related muscle loss can be characterized as decrease of muscle mass, motor unit, and muscle cross sectional area, and finally decrease of muscle quality 1, 2, 3. Muscle loss in particular can lead to a decrease of functional capacity of muscle and increase risk of developing age-related problems, such as chronic diseases 4 …”

Section: Introductionmentioning

confidence: 99%

“…With one of the muscle's important characteristics, plasticity, 3 muscle can adapt to environment throughout life, and trainability of skeletal muscle and muscle improvement has been demonstrated in aging 6 . Also, exercise accelerates production of various cytokines and growth factors or regulates homeostasis 5 .…”

Section: Introductionmentioning

confidence: 99%

Resistance exercise reduced the expression of fibroblast growth factor-2 in skeletal muscle of aged mice

Yoon

Kim

Choi

et al. 2016

Integrative Medicine Research

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BackgroundFibroblast growth factor-2 (FGF-2) protein level has been shown to be elevated in aged mice muscle compared to adult mice. It activates the satellite cell quiescence, which leads to satellite cell depletion and may accelerate aging process. The purpose of this study was to see the effect of resistance exercise on skeletal muscle FGF-2 protein level in aged mice.MethodsThis study included eight young adult control C57BL/6 male mice (age 12 weeks, YCON group) and 14 aged C57BL/6 male mice (age 19 months), randomly divided into two groups (old control, OCON, n = 7; old resistance exercise, ORT, n = 7). Resistance ladder climbing exercise was conducted 3 d/wk for 12 weeks. Soleus and tibialis anterior muscles were collected for body composition, relative grip strength, and muscle wet weight and for enzyme-linked immunosorbent assay protein analysis.ResultsRelative soleus muscle wet weight and hindlimb lean mass showed a significant increase in ORT group compared to OCON group (p = 0.013 and p = 0.015, respectively). In relative grip strength, both OCON and ORT showed a significant decrease compared to YCON (p < 0.001 and p = 0.011, respectively). However, ORT showed a significant increase compared to OCON (p = 0.02). OCON showed a significant increase in skeletal muscle FGF-2 protein level compared to YCON in soleus (p = 0.035), and ORT showed a significant decrease compared to OCON in soleus muscle (p = 0.045). FGF-2 protein level was significantly decreased in tibialis anterior muscle in the ORT group compared to OCON (p = 0.022). Correlation analysis showed a negative correlation between FGF-2 protein level and soleus and tibialis anterior muscle weight (r2 = 0.514, p = 0.0035; r2 = 0.312, p = 0.025, respectively).ConclusionTwelve weeks of resistance exercise reduced soleus and tibialis anterior muscle FGF-2 protein level in aged mice with improvement in appendicular lean mass and muscle strength. Thus, resistance exercise could be an efficient intervention for preventing age-related increase of FGF-2 in skeletal muscle.

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Muscle Changes in Aging

Cited by 139 publications

References 40 publications

3D-printed drug testing platform based on a 3D model of aged human skeletal muscle

3D-printed drug testing platform based on a 3D model of aged human skeletal muscle

Sarcopenia and its association with falls and fractures in older adults: A systematic review and meta‐analysis

Resistance exercise reduced the expression of fibroblast growth factor-2 in skeletal muscle of aged mice

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