Muscle extract infusion in rabbits. A new experimental model of the crush syndrome.

Abstract

SUMMARY Previous studies provide inconclusive data concerning the nephrotoxicity of myoglobin following muscle injury. We investigated the possibility that released muscle constituents other than myoglobin may be associated with renal damage, and studied accompanying hematological and coagulation changes. An extract of homologous or autologous muscle was infused intravenously in rabbits in a dose of 100 mg of muscle extract protein/kg; equine myoglobin was given to control animal*. Experimental animals developed proteinuria, cylindruria, and a 50% reduction in glomemlar filtration rate. Leukopenia, thrombocytopenia and evidence of intravascular coagulation also were seen. The muscle extract was shown to have thromboplastlc activity; however inhibition of this by phospholipase C did not prevent the changes induced by muscle extract infusion possibly because the intrinsic coagulation pathway still was activated. Although moderate hypotension and ECG changes developed in some rabbits, these were not consistent and the renal functional changes appeared to be independent of these factors. Pulmonary and glomerular microthrombi were seen in experimental animals and there was vacuolation of the renal proximal tubular cells. These studies indicate that a number of biological systems are activated following muscle extract infusion and that these may be more important than the nephrotoxicity of myoglobin in the pathogenesis of the renal injury. Ore Res 49:114-124, 1981

Hyperosmolal State Associated with Rhabdomyolysis

“…The develop ment of acute tubular necrosis was probably prevented by timely replacement of fluid deficit and the ongoing solute diuresis (glycosuria). The patient also developed thrombocytopenia and elevation of fibrin split products, consistent with disseminated intravascular coagulation, a frequently observed complication in patients with rhab domyolysis [5], A rise in the serum sodium concentration was noted despite the administration of large amounts of 0.5 N saline. This can be attributed to the fall in the serum glucose level and the associated shifts in water to the intracellular space.…”

Central Hemodynamic Changes in Experimental Muscle Crush Injury in Pigs

“…The effects of muscle extract infusion vary widely according to dosage, infusion technique and other model design variables. O'Regan et al [9] observed a mild vasodepressor effect after a 20-min infusion of 10 mg allogenic muscle extract protein/100 g body weight in rats, whereas in the study by Blachar et al [13] a similar allogenic dose injected as a bolus in rabbits caused a 40% immediate decrease in systolic and diastolic blood pressure. While reactive oxygen-carrying species (i.e.…”

Expressão de citoquinas plasmáticas após compressão de membros inferiores de ratos

“…7,8,[12][13][14][15] Com nosso modelo, os resultados demonstraram alteraç ões significantes dos níveis de IL 10 após quatro horas da liberação da compressão. Embora os resultados mais reprodutíveis tenham sido encontrados em modelos experimentais, ensaios clínicos relacionam os níveis de citoquinas plasmáticas e a gravidade de pacientes com múltiplas lesões traumáticas.…”