Muscle fiber typing in routinely processed skeletal muscle with monoclonal antibodies

Havenith, M. G.; Visser, R.; Schendel, J. M. C. Schrijvers-Van; Bosman, Fred T.

doi:10.1007/bf00266407

Cited by 71 publications

(36 citation statements)

References 12 publications

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“…MHC isoforms of skeletal muscle fibers correspond to certain types of muscle fibers determined by the ordinary enzyme histochemical classification [37,39,56,76,78,79] as shown in Figs. 1 and 2.…”

Section: Classical Identification Of Muscle Fibermentioning

confidence: 96%

Immunohistochemical Classification of Skeletal Muscle Fibers.

Hori¹,

Ishihara

Kobayashi³

et al. 1998

Acta Histochem. Cytochem

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Section: Classical Identification Of Muscle Fibermentioning

confidence: 96%

Immunohistochemical Classification of Skeletal Muscle Fibers.

Hori¹,

Ishihara

Kobayashi³

et al. 1998

Acta Histochem. Cytochem

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“…The specificity and characterization of these mAbs against MHC isoforms have been previously established. The mAbs NOQ7-5-4D is specific to type I MHC (Narusawa et al, 1987;Petrof et al, 1992;Rubinstein and Hoh, 2000), MY-32 to all fast-type MHCs (Havenith et al, 1990;Monemi et al, 2000), SC-71 to type IIa MHC (Schiaffino et al, 1989;Stal et al, 1994;Monemi et al, 2000), ALD-58 to MHC-ton (Shafiq et al, 1984;, BA-G5 to MHC-␣ (Michel et al, 1996), F1.652 to MHC-emb (Michel et al, 1996), and N2.261 to MHC-neo (Michel et al, 1996).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Adult human mylohyoid muscle fibers express slow‐tonic, α‐cardiac, and developmental myosin heavy‐chain isoforms

Wang

et al. 2004

Anat. Rec.

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Some adult cranial muscles have been reported to contain unusual myosin heavy-chain (MHC) isoforms (i.e., slow-tonic, ␣-cardiac, embryonic, and neonatal), which exhibit distinct contractile properties. In this study, adult human mylohyoid (MH) muscles obtained from autopsies were investigated to detect the unusual MHC isoforms. For comparison, the biceps brachii and masseter muscles of the same subjects were also examined. Serial cross-sections from the muscles studied were incubated with a panel of isoform-specific anti-MHC monoclonal antibodies that distinguish major and unusual MHC isoforms. On average, the slow type I and fast type II MHC-containing fibers in the MH muscle accounted for 54% and 46% of the fibers, respectively. In contrast to limb and trunk muscles, the adult human MH muscle was characterized by a large proportion of hybrid fibers (85%) and a small percentage of pure fibers (15%; P Ͻ 0.01). Of the fast fiber types, the proportion of the type IIa MHC-containing fibers (92%) was much greater than that of the type IIx MHC-containing fibers (8%; P Ͻ 0.01). Our data demonstrated that the adult human MH fibers expressed the unusual MHC isoforms that were also identified in the masseter, but not in the biceps brachii. These isoforms were demonstrated by immunocytochemistry and confirmed by electrophoretic immunoblotting. Fiber-to-fiber comparisons showed that the unusual MHC isoforms were coexpressed with the major MHC isoforms (i.e., MHCI, IIa, and IIx), thus forming various major/unusual (or m/u) MHC hybrid fiber types. Interestingly, the unusual MHC isoforms were expressed in a fiber type-specific manner. The slow-tonic and ␣-cardiac MHC isoforms were coexpressed predominantly with slow type I MHC isoform, whereas the developmental MHC isoforms (i.e., embryonic and neonatal) coexisted primarily with fast type IIa MHC isoform. There were no MH fibers that expressed exclusively unusual MHC isoforms. Approximately 81% of the slow type I MHC-containing fibers expressed slow-tonic and ␣-cardiac MHC isoforms, whereas 80% of the fast type IIa MHC-containing fibers expressed neonatal MHC isoform. The m/u hybrid fibers (82% of the total fiber population) were found to constitute the predominant fiber types in the adult human MH muscle. At least seven m/u MHC hybrid fiber types were identified in the adult human MH muscle. The most common m/u hybrid fiber types were found to be the MHCI/slow-tonic/␣-cardiac and MHCIIa/neonatal, which accounted for 39% and 33% of the total fiber population, respectively. The multiplicity of MHC isoforms in the adult MH fibers is believed to be related to embryonic origin, innervation pattern, and unique functional requirements.

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“…The MY-32 antibody (Havenith et al, 1990) has been shown to specifically identify type IIA and IIB muscle fibers. MY-32 immunoreactivity was localized to muscle fibers that were both parallel and perpendicular to the plane of the tissue (Fig.…”

Section: Newborn and Adult Tongue Muscles Are Composed Exclusively Ofmentioning

confidence: 99%

“…The MY-32 monoclonal antibody (Sigma Chemical Co., St. Louis, MO) has been shown to specifically label type II skeletal muscle fibers (Havenith et al, 1990). To attain a high-resolution image of MY-32-labeled thick filaments, the images were recorded photographically and the color print was scanned such that the contrast could be adjusted for printing on a Phaser 440 high-resolution color printer.…”

Section: Immunohistochemistrymentioning

confidence: 99%