Muscle growth in Nile tilapia (Oreochromis niloticus): histochemical, ultrastructural and morphometric study

Pai-Silva, Maeli Dal; Carvalho, Robson Francisco; Pellizzon, Cláudia Helena; Pai, Vitalino Dal

doi:10.1016/s0040-8166(03)00019-3

Cited by 27 publications

(17 citation statements)

References 37 publications

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“…The large red fibers of the adductor pectoral fin muscle of M. furnieri presented the same metabolic features described for the "other red" fiber type of the myotomal musculature of this species, and species such as Oreochromis niloticus (Linnaeus, 1758) and Thunnus orientalis (Temminck & Schlegel, 1844) (Dal pai-silva et al 2003, rOy et al 2012). On the other hand, the activity of the mATPase exhibited a pattern opposite to the one found in the myotomal and abductor muscles (Devincenti et al 2000a(Devincenti et al , 2009).…”

Section: 10 11supporting

confidence: 63%

The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

Hernández¹,

Longo²,

Devincenti³

et al. 2016

Zoologia (Curitiba)

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ABSTRACT. The adductor pectoral fin muscle in post-spawning females of the white croaker Micropogonias furnieri (Desmarest, 1823) is described from a morphological, histochemical and morphometric perspectives. A description of the morphological characteristics was conducted after dissections in different layers, down to the deep layer. Five muscles were found: superficial, medial, radial and deep adductors, and dorsal arrector. Their fibers were studied after applying histochemical techniques: succinic dehydrogenase (SDH) to reveal mitochondria, periodic acid-Schiff (PAS) for glycogen, Sudan black for lipids, myosin adenosinetriphosphatase (mATPase) to reveal the types of fibers, and modified mATPase to evidence the capillaries. Fiber diameters were measured and the number of capillaries was counted. Fiber subtypes named small, medium and large were found within red, pink and white fibers, the latter prevailing. Staining homogeneity was observed in white fibers after alkaline pre-incubations. The number of capillaries decreased from red to white fibers. Due to the prevalence of white fibers, the adductor muscle of the pectoral fins appears to be capable of rapid and discontinuous movements, which are important to body stabilization during subcarangiform swimming. The homogenous staining of white fibers observed in this research corresponds to the post-spawning gonad stage studied.

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Section: 10 11supporting

confidence: 63%

The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

Hernández¹,

Longo²,

Devincenti³

et al. 2016

Zoologia (Curitiba)

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“…Proliferation ceases as the animal grows and muscle fibers gain volume. In other words, a greater number of muscle fibers with greater diameters may be observed in adult animals as compared to juveniles (Dal Pai-Silva et al, 2003).…”

Section: Discussionmentioning

confidence: 97%

Effect of propolis ethanol extract on myostatin gene expression and muscle morphometry of Nile tilapia in net cages

Buck¹,

Mizubuti

Alfieri

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Propolis can be used as growth enhancer due to its antimicrobial, antioxidant, and immune-stimulant properties, but its effects on morphometry and muscle gene expression are largely unknown. The present study evaluates the influence of propolis on muscle morphometry and myostatin gene expression in Nile tilapia (Oreochromis niloticus) bred in net cages. Reversed males (GIFT strain) with an initial weight of 170 ± 25 g were distributed in a (2 x 4) factorial 2 E.L. Buck et al.Genetics and Molecular Research 16 (1): gmr16019404 scheme, with two diets (DPRO, commercial diet with 4% propolis ethanol extract and DCON, commercial diet without propolis, control) and four assessment periods (0, 35, 70, and 105 experimental days). Muscles were evaluated at each assessment period. Histomorphometric analysis classified the fiber diameters into four groups: <20 mm; 20-30 mm; 30-50 mm; and > 50 mm. RT-qPCR was performed to assess myostatin gene expression. Fibers < 20 µm diameter were more frequent in DPRO than in DCON at all times. Fiber percentages >30 µm (30-50 and > 50 µm) at 70 days were 25.39% and 40.07% for DPRO and DCON, respectively. There was greater myostatin gene expression at 105 days, averaging 1.93 and 1.89 for DCON and DPRO, respectively, with no significant difference in any of the analyzed periods. Propolis ethanol extract did not affect the diameter of muscle fibers or the gene expression of myostatin. Future studies should describe the mechanisms of natural products' effects on muscle growth and development since these factors are highly relevant for fish production performance.

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“…Either white or red muscle fibers are recruited depending on the distinct needs of the organism regarding the use of skeletal muscle. Specifically, the red fibers have slow contractions and oxidative metabolisms and are associated with slow movements that are related to foraging and migrations habits, whereas the white fibers have fast contractions and glycolytic metabolisms and are associated with higher-speed movements that are related to escape and to the capture of food [47–49]. In contrast to these morphophysiological differences between the two types of muscle, the present study did not find any significant differences between the expression patterns of MRF transcripts in the white muscles comparing to the red muscles in clear water fish.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Myogenic Regulatory Factor Genes in the Skeletal Muscles of TambaquiColossoma macropomum(Cuvier 1818) from Amazonian Black and Clear Water

Alves-Costa

Barbosa

Aguiar

et al. 2013

International Journal of Genomics

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Hypothesizing that the Amazonian water system differences would affect the expression of muscle growth-related genes in juvenile tambaqui Colossoma macropomum (Cuvier 1818), this study aimed to analyze the morphometric data and expression of myogenic regulatory factors (MRFs) in the white and red muscle from tambaqui obtained from clear and black Amazonian water systems. All of the MRF transcript levels (myod, myf5, myogenin, and mrf4) were significantly lower in the red muscle from black water fish in comparison to clear water fish. However, in white muscle, only the myod transcript level was significantly decreased in the black water tambaqui. The changes in MRFs gene expression in muscle fibers of tambaqui from black water system provide relevant information about the environmental influence as that of water systems on gene expression of muscle growth related genes in the C. macropomum. Our results showed that the physical and chemical water characteristics change the expression of genes that promote muscle growth, and these results may be also widely applicable to future projects that aim to enhance muscle growth in fish that are of substantial interest to the aquaculture.

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Muscle growth in Nile tilapia (Oreochromis niloticus): histochemical, ultrastructural and morphometric study

Cited by 27 publications

References 37 publications

The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

Effect of propolis ethanol extract on myostatin gene expression and muscle morphometry of Nile tilapia in net cages

Differential Expression of Myogenic Regulatory Factor Genes in the Skeletal Muscles of TambaquiColossoma macropomum(Cuvier 1818) from Amazonian Black and Clear Water

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