Muscle mitochondrial capacity in high‐ and low‐fitness females using near‐infrared spectroscopy

Lagerwaard, Bart; Janssen, Joëlle J. E.; Cuijpers, Iris; Keijer, Jaap; Boer, Vincent C.J. de; Nieuwenhuizen, Arie G.

doi:10.14814/phy2.14838

Cited by 12 publications

(17 citation statements)

References 34 publications

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“…Consistent with the current results, we suspect that the lower metabolic rate and lower O 2 affinity of females was reflected as a slower rate of O 2 extraction as compared to the males during a period of arterial occlusion. In support of our NIRS-derived mitochondrial-related hypothesis, it has recently been demonstrated that NIRS measurements were capable of providing indices of mitochondria capacity in females (Lagerwaard et al 2021).Therefore, the current results provide an extension to previous findings (Fellahi et al 2014;Beltrame et al 2017;Miotto et al 2018;Cardinale et al 2018Cardinale et al , 2019Ansdell et al 2019;Lagerwaard et al 2021), such that females have slower O 2 extraction rates compared to males likely prompted by differences in the proportional areas of fiber types as well as mitochondrial function.…”

Section: Early Phase Skeletal Muscle Tissue Desaturation Ratesupporting

confidence: 88%

Men exhibit faster skeletal muscle tissue desaturation than women before and after a fatiguing handgrip

Keller

Kennedy

2021

Eur J Appl Physiol

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Purpose The purpose was to test the hypothesis that sex and fatigue effect of the early phase of skeletal muscle tissue oxygenation (StO 2 , %) desaturation rate as well as that strength matched adults may exhibit similar responses. Methods Twenty-four adults visited the laboratory twice to quantify this early phase of desaturation during vascular occlusion tests (VOT) while in a rested state. The second visit included a sustained handgrip task at 25% of maximal muscular strength until task failure. At failure, a post-task VOT was initiated. Muscle desaturation was defined as StO 2 and collected by a near-infrared spectroscopy device. The muscle size and adipose thickness were determined via ultrasonography. Linear regression was used to quantify the rates of desaturation during the VOTs as well as during the fatiguing handgrip. Results There were sex differences in the rate of desaturation pre- and post-handgrip, such that independent of fatigue, the men ( p < 0.001) desaturated more rapidly than the women (pre: b = − 0.208 vs. − 0.123%∙s −1 ; post: − 0.079 vs. − 0.070%∙s −1 ). During the fatiguing handgrip, the transformed StO 2 values indicated that the males desaturated more rapidly than the females ( b = − 0.070 vs. − 0.015). The matched pairs exhibited the same responses as the total sample. Conclusion Overall, muscle size and strength as well as adipose tissue were likely not the primary cause of the differences in rates of muscle desaturation. We hypothesized that differences in fiber type and mitochondria were the principle mechanisms provoking the differences in muscle oxygenation.

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Section: Early Phase Skeletal Muscle Tissue Desaturation Ratesupporting

confidence: 88%

Men exhibit faster skeletal muscle tissue desaturation than women before and after a fatiguing handgrip

Keller

Kennedy

2021

Eur J Appl Physiol

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“…The original objectives of the study were to investigate whether differences exist in vitamin B2 status, in muscle mitochondrial capacity, and in circulating cellular and molecular parameters between high aerobically fit (high-fit) and low aerobically fit (low-fit) females with a validated difference in V̇O 2 peak, and after a recent bout of exercise. Previously, we found a significant difference in skeletal muscle mitochondrial oxygen consumption rate [ 21 ] and mitochondrial function in white blood cells [ 22 ] between the high-fit and low-fit females in our healthy study population. Circulating biomarker and metabolic parameter analysis in this study will now show whether single biomarkers, functional biomarkers, metabolic parameter categories, total biomarkers, and metabolic parameter profiles differ between high-fit and low-fit females, i.e., whether the biomarkers and metabolic parameter, including those that discriminate between health and disease, already differ in healthy individuals with potentially a different long term disease risk, in the basal state and after a recent bout of exercise.…”

Section: Introductionmentioning

confidence: 82%

Single and Joined Behaviour of Circulating Biomarkers and Metabolic Parameters in High-Fit and Low-Fit Healthy Females

Janssen

Lagerwaard

Nieuwenhuizen

et al. 2023

IJMS

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Biomarkers are important in the assessment of health and disease, but are poorly studied in still healthy individuals with a (potential) different risk for metabolic disease. This study investigated, first, how single biomarkers and metabolic parameters, functional biomarker and metabolic parameter categories, and total biomarker and metabolic parameter profiles behave in young healthy female adults of different aerobic fitness and, second, how these biomarkers and metabolic parameters are affected by recent exercise in these healthy individuals. A total of 102 biomarkers and metabolic parameters were analysed in serum or plasma samples from 30 young, healthy, female adults divided into a high-fit (V̇O2peak ≥ 47 mL/kg/min, N = 15) and a low-fit (V̇O2peak ≤ 37 mL/kg/min, N = 15) group, at baseline and overnight after a single bout of exercise (60 min, 70% V̇O2peak). Our results show that total biomarker and metabolic parameter profiles were similar between high-fit and low-fit females. Recent exercise significantly affected several single biomarkers and metabolic parameters, mostly related to inflammation and lipid metabolism. Furthermore, functional biomarker and metabolic parameter categories corresponded to biomarker and metabolic parameter clusters generated via hierarchical clustering models. In conclusion, this study provides insight into the single and joined behavior of circulating biomarkers and metabolic parameters in healthy females, and identified functional biomarker and metabolic parameter categories that may be used for the characterisation of human health physiology.

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“…The use of oral contraceptives was not excluded; only the use of monophasic oral contraceptives containing low synthetic estradiol and progesterone was allowed and was controlled for ( n = 7 in the high-fit and n = 6 in the low-fit group). Subjects were selected if they had a V̇ o 2peak ≥ 47 mL/kg/min (high-fit group) or ≤37 mL/kg/min (low-fit group) determined using a maximal exercise test, measured using the validated screening protocol of Lagerwaard et al ( 30 , 31 ), which minimized the risk for selective bias. The V̇ o 2peak cut-offs were based on previous findings from our laboratory in high-fit (trained) and low-fit (untrained) males ( 31 ) and previous studies in high-fit (trained) and low-fit (untrained) females ( 32 – 34 ).…”

Section: Methodsmentioning

confidence: 99%

“…For sufficient power, we focused on one sex, because PBMC metabolism was found to differ between males and females ( 35 ) and previous exercise studies have been mainly performed in males ( 36 – 38 ). The V̇ o 2peak data and results of skeletal muscle mitochondrial capacity of these included subjects have been published previously by our group ( 30 ).…”

Section: Methodsmentioning

confidence: 99%

Extracellular flux analyses reveal differences in mitochondrial PBMC metabolism between high-fit and low-fit females

Janssen

Lagerwaard

Porbahaie

et al. 2022

American Journal of Physiology-Endocrinology and Metabolism

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Analyzing metabolism of peripheral blood mononuclear cells (PBMCs) can possibly serve as a cellular metabolic read-out for lifestyle factors and lifestyle interventions. However, the impact of PBMC composition on PBMC metabolism is not yet clear, neither is the differential impact of a longer-term lifestyle factor versus a short-term lifestyle intervention. We investigated the effect of aerobic fitness level and a recent exercise bout on PBMC metabolism in females. PBMCs from 31 young female adults divided into a high-fit (V̇O2peak ≥ 47 mL/kg/min, N = 15) and low-fit (V̇O2peak ≤ 37 mL/kg/min, N = 16) group were isolated at baseline and overnight after a single bout of exercise (60 minutes, 70% V̇O2peak). Oxygen consumption rate (OCR) and glycolytic rate (GR) were measured using extracellular flux (XF) assays and PBMC subsets were characterized using fluorescence-activated cell sorting (FACS). Basal OCR, FCCP-induced OCR, spare respiratory capacity, ATP-linked OCR, and proton leak were significantly higher in high-fit compared to low-fit females (all P < 0.01), while no significant differences in glycolytic rate (GR) were found (all P > 0.05). A recent exercise bout did not significantly affect GR or OCR parameters (all P > 0.05). The overall PBMC composition was similar between high-fit and low-fit females. Mitochondrial PBMC function was significantly higher in PBMCs from high-fit compared to low-fit females, which was unrelated to PBMC composition and not impacted by a recent bout of exercise. Our study reveals a link between PBMC metabolism and levels of aerobic fitness, increasing the relevance of PBMC metabolism as a marker to study the impact of lifestyle factors on human health.

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Muscle mitochondrial capacity in high‐ and low‐fitness females using near‐infrared spectroscopy

Cited by 12 publications

References 34 publications

Men exhibit faster skeletal muscle tissue desaturation than women before and after a fatiguing handgrip

Men exhibit faster skeletal muscle tissue desaturation than women before and after a fatiguing handgrip

Single and Joined Behaviour of Circulating Biomarkers and Metabolic Parameters in High-Fit and Low-Fit Healthy Females

Extracellular flux analyses reveal differences in mitochondrial PBMC metabolism between high-fit and low-fit females

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