Muscope: a miniature on-chip lensless microscope

Prajapati, Ekta; Kumar, Sachin; Kumar, Sunil

doi:10.1039/d1lc00792k

Cited by 8 publications

(4 citation statements)

References 46 publications

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“…In addition, the research presented in this work paves the way for the development of miniaturized microscopes [ 80 , 81 , 82 , 83 , 84 ] based on fluorescence, the gold standard tool used in biology. This promising advancement is envisaged through the utilization of large arrays integrating smaller LEDs, complemented by the appropriate driving circuits.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array

Moro,

Canals,

Moreno

et al. 2024

Biosensors

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A Point-of-Care system for molecular diagnosis (PoC-MD) is described, combining GaN and CMOS chips. The device is a micro-system for fluorescence measurements, capable of analyzing both intensity and lifetime. It consists of a hybrid micro-structure based on a 32 × 32 matrix addressable GaN microLED array, with square LEDs of 50 µm edge length and 100 µm pitch, with an underneath wire bonded custom chip integrating their drivers and placed face-to-face to an array of 16x16 single-photon avalanche diodes (SPADs) CMOS. This approach replaces instrumentation based on lasers, bulky optical components, and discrete electronics with a full hybrid micro-system, enabling measurements on 32 × 32 spots. The reported system is suitable for long lifetime (>10 ns) fluorophores with a limit of detection ~1/4 µM. Proof-of-concept measurements of streptavidin conjugate Qdot™ 605 and Amino PEG Qdot™ 705 are demonstrated, along with the device ability to detect both fluorophores in the same measurement.

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Section: Discussionmentioning

confidence: 99%

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array

Moro,

Canals,

Moreno

et al. 2024

Biosensors

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“…Herein, we present a multimodal sensing CMOS imager (M‐imager) based on a dual‐focus imaging strategy that combines traditional lens‐based imaging for visual information acquisition with lensless imaging to integrate other sensing modalities. [ 16 ] Mimicking the multimodal information collection of mosquitoes during host location, we printed colorimetric sensing materials on the imager surface to detect carbon dioxide (CO 2 ), temperature, and humidity. These sensing units and visual information can be captured in a single image, which enables extracting more effective characteristics without dimensional correlation and facilitates further data processing and analysis.…”

Section: Introductionmentioning

confidence: 99%

A Multimodal Sensing CMOS Imager Based on Dual‐Focus Imaging

et al. 2023

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Advanced machine intelligence is empowered not only by the ever‐increasing computational capability for information processing but also by sensors for collecting multimodal information from complex environments. However, simply assembling different sensors can result in bulky systems and complex data processing. Herein, it is shown that a complementary metal‐oxide‐semiconductor (CMOS) imager can be transformed into a compact multimodal sensing platform through dual‐focus imaging. By combining lens‐based and lensless imaging, visual information, chemicals, temperature, and humidity can be detected with the same chip and output as a single image. As a proof of concept, the sensor is equipped on a micro‐vehicle, and multimodal environmental sensing and mapping is demonstrated. A multimodal endoscope is also developed, and simultaneous imaging and chemical profiling along a porcine digestive tract is achieved. The multimodal CMOS imager is compact, versatile, and extensible and can be widely applied in microrobots, in vivo medical apparatuses, and other microdevices.

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“…The requirement for thin microfluidic chips also arises from replacing the traditional bulky and expensive optical microscopes with their lensless counterparts, particularly Muscope [4]. Muscope is a miniature on-chip digital in-line holographic microscope (DIHM) [5,6], reported by us -has a form factor of about 4 mm and can be easily integrated within larger systems.…”

Section: Introductionmentioning

confidence: 99%

Thin Microfluidic Chips with Active Valves

Prajapati

Giri

Rath

et al. 2023

Preprint

Self Cite

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We report the fabrication of very thin microfluidic active and passive devices on rigid and flexible substrates for sample-space-restricted applications. Thin glass coverslips are commonly used substrates, but these being fragile often crack during experiments, leading to device failure. Here, we used PET as a flexible substrate to fabricate robust thin devices. We proposed a simpler process for PET-PDMS bonding without any silane, adhesive, and/or plasma treatment. We presented the compatibility of the thin devices with a digital in-line holographic microscope (DIHM) as a use case. The substitution of the conventional microscope with DIHM in microfluidic large-scale integrated systems renders simplicity, cost effectiveness, portability, and miniaturization of the overall system. It also enables a customized and parallel multisite optical observation for a complex microfluidic circuit chip. These chips comprise various microfluidic components made of active microvalves, particularly Quake valves. We also successfully demonstrated the function of microvalves fabricated with our method to regulate the fluidic flow. Thus, are suited to making sophisticated microfluidic circuit chips to fit a variety of applications like organ-on-chip, cell culture, wearable biosensors, pressure sensors, etc.

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Muscope: a miniature on-chip lensless microscope

Abstract: We report Muscope, a miniature lensless holographic microscope suitable for on-chip integration. The prototype of Muscope measured approximately only 7 mm x 4 mm x 4 mm, yet was capable...

Cited by 8 publications

References 46 publications

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array

A Multimodal Sensing CMOS Imager Based on Dual‐Focus Imaging

Thin Microfluidic Chips with Active Valves

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