Musculoskeletal Tissue Engineering: Tendon, Ligament, and Skeletal Muscle Replacement and Repair

Uquillas, Jorge A.; Pacelli, Settimio; Kobayashi, Shuichiro; Uquillas, Sebastián

doi:10.1002/9783527689934.ch15

Cited by 4 publications

(5 citation statements)

References 332 publications

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“…According to this, tendons are fibrous tissues that join skeletal muscle to bone, making movements possible through force transmission from muscles to bones [ 220 ]. At the same time, ligaments are the dense fibrous connective tissue that connects bone to bone [ 221 ]. The transmission of these tensile forces by tendons and ligaments makes them susceptible to tearing or complete rupture, depending on the amount of the force [ 222 ].…”

Section: Tendon and Ligamentmentioning

confidence: 99%

“…The chemical composition of the tendon and ligament is very similar, with a slight difference in the number of components. The main component of both tendon and ligament is water, 60% to 80% in weight [ 220 , 221 ]. They contain a protein phase (collagen) and a polysaccharide phase (proteoglycans).…”

Section: Tendon and Ligamentmentioning

confidence: 99%

“…The other 5% involves mainly collagen types III and V. There are minimal amounts of collagen types II, VI, IX, X, and XI. On the other hand, ligaments contain more protein, less total collagen, and greater amounts of type III collagen and GAGs [ 221 ]. While many of these collagen types’ full biological and biophysical roles are still unclear, some specific functions of each type have been identified [ 224 ].…”

Section: Tendon and Ligamentmentioning

confidence: 99%

“…They are the predominant cell type in the tendon that can mature into tenocytes with fibroblastic morphology and low metabolic activity. Other types of cells present in the tendon are progenitor cells, synovial cells, endothelial cells, and even chondrocytes [ 221 ]. The primary cell type of ligament is fibroblasts, and these cells help in the production of collagen and matrix remodeling by the degradation of the pre-existing collagen [ 225 ].…”

Section: Tendon and Ligamentmentioning

confidence: 99%

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A Review of Recent Advances in Natural Polymer-Based Scaffolds for Musculoskeletal Tissue Engineering

et al. 2022

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The musculoskeletal (MS) system consists of bone, cartilage, tendon, ligament, and skeletal muscle, which forms the basic framework of the human body. This system plays a vital role in appropriate body functions, including movement, the protection of internal organs, support, hematopoiesis, and postural stability. Therefore, it is understandable that the damage or loss of MS tissues significantly reduces the quality of life and limits mobility. Tissue engineering and its applications in the healthcare industry have been rapidly growing over the past few decades. Tissue engineering has made significant contributions toward developing new therapeutic strategies for the treatment of MS defects and relevant disease. Among various biomaterials used for tissue engineering, natural polymers offer superior properties that promote optimal cell interaction and desired biological function. Natural polymers have similarity with the native ECM, including enzymatic degradation, bio-resorb and non-toxic degradation products, ability to conjugate with various agents, and high chemical versatility, biocompatibility, and bioactivity that promote optimal cell interaction and desired biological functions. This review summarizes recent advances in applying natural-based scaffolds for musculoskeletal tissue engineering.

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Section: Tendon and Ligamentmentioning

confidence: 99%

Section: Tendon and Ligamentmentioning

confidence: 99%

Section: Tendon and Ligamentmentioning

confidence: 99%

Section: Tendon and Ligamentmentioning

confidence: 99%

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A Review of Recent Advances in Natural Polymer-Based Scaffolds for Musculoskeletal Tissue Engineering

et al. 2022

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“…Among all the strategies to manage these kind of injuries tissue engineering is very promising, as it allows regeneration of the native tissue (Santos et al ., ). Thanks to its capability to produce nanofibres from polymeric solutions of both resorbable and biostable materials, electrospinning is one of the most promising technologies in the field of regenerative medicine (Uquillas et al ., ). In fact, electrospinning technology allows to fabricate scaffolds that reproduce the structure of collagen fibrils (Verdiyeva et al ., ).…”

Section: Introductionmentioning

confidence: 97%

High‐resolution x‐ray tomographic morphological characterisation of electrospun nanofibrous bundles for tendon and ligament regeneration and replacement

Sensini

Cristofolini

Belcari

et al. 2018

Journal of Microscopy

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Repair of ligaments and tendons requires scaffolds mimicking the spatial organisation of collagen in the natural tissue. Electrospinning is a promising technique to produce nanofibres of both resorbable and biostable polymers with desired structural and morphological features. The aim of this study was to perform high-resolution x-ray tomography (XCT) scans of bundles of Nylon6.6, pure PLLA and PLLA-Collagen blends, where the nanofibres were meant to have a predominant direction. Characterisation was carried out via a dedicated methodology to firmly hold the specimen during the scan and a workflow to quantify the directionality of the nanofibres in the bundle. XCT scans with 0.4 and 1.0 μm voxel size were successfully collected for all bundle compositions. Better image quality was achieved for those bundles formed by thicker nanofibres (i.e. 0.59 μm for pure PLLA), whereas partial volume effect was more pronounced for thinner nanofibres (i.e. 0.26 μm for Nylon6.6). As expected, the nanofibres had a predominant orientation along the axis of the bundles (more than 20% of the nanofibres within 3° and more than 60% within 18° from the bundle axis), with a Gaussian-like dispersion in the other directions. The directionality assessment was validated by comparison against a similar analysis performed on SEM images: the XCT analysis overestimated the amount of nanofibres very close to the bundle axis, especially for the materials with thinnest nanofibres, but adequately identified the amount of nanofibres within 12°. LAY DESCRIPTION: Repair of ligaments and tendons requires dedicated materials (scaffolds) mimicking the spatial organisation of the collagen (the main material composing such natural tissue). Electrospinning is a promising technique that allows production of fibres with nanometric dimension using high voltage to stretch very tiny drops of polymeric solutions. Electrospinning allows processing both polymers that can be resorbed by the host tissue, and nonresorbable ones, to obtain the desired structural and morphological features by arranging the nanofibres in bundles. The aim of this study was to perform high-resolution x-ray computed tomography (XCT) scans of bundles, where the nanofibres were meant to have a predominant direction. The investigation included bundles of different compositions: a biostable polymer (Nylon) and bioresorbable ones (pure Poly-L-lactic acid (PLLA) and PLLA-Collagen blends). The electrospun bundles were produced using a validated method (Sensini et al 2017: https://doi.org/10.1088/1758-5090/aa6204). To this end, we developed a dedicated methodology to scan such small specimens, and a workflow to quantify the directionality of the nanofibres in the bundle. For all the compositions, XCT scans with extremely high resolution (i.e. down to 0.4 μm) were successfully collected. As expected, better images were obtained for those bundles where the nanofibres were thicker than the scanning resolution (i.e. 0.59 μm for pure PLLA). The images of the thinnest nanofibres (i.e. 0.26 μm for Nylon)...

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Protein-Based Scaffolds for Musculoskeletal Tissue Repair and Regeneration

Rashidi,

Tamaddon,

Liu

2023

Handbook of the Extracellular Matrix

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Musculoskeletal Tissue Engineering: Tendon, Ligament, and Skeletal Muscle Replacement and Repair

Cited by 4 publications

References 332 publications

A Review of Recent Advances in Natural Polymer-Based Scaffolds for Musculoskeletal Tissue Engineering

A Review of Recent Advances in Natural Polymer-Based Scaffolds for Musculoskeletal Tissue Engineering

High‐resolution x‐ray tomographic morphological characterisation of electrospun nanofibrous bundles for tendon and ligament regeneration and replacement

Protein-Based Scaffolds for Musculoskeletal Tissue Repair and Regeneration

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