Objectives: The present study aim to identify and determine the resistance profile of Staphylococcus coagulase-negative (SCN) and Staphylococcus coagulase-positive (SCP) strains isolated from stethoscopes of nursing students at a public university in Pernambuco. Methods: Samples were collected from 60 students’ stethoscopes and seeded in culture medium supplemented with oxacillin (6 µg/mL) e vancomycin (6 µg/mL) to screen for detection of resistant strains. Subsequently, it was performed the identification of the isolates through breeding in agar Baird Parker and biochemical tests. To determine the resistance profile, disk diffusion tests, “D” test and broth microdilution tests were performed.Results: Among the analyzed strains in the present study, one SCN strain presented resistance to oxacillin and vancomycin and another strain showed intermediate resistance to vancomycin, and resistance to oxacillin with positive “D” test. In addition, other four SCP exhibited total resistance to more than one drug. Conclusions: In brief, the importance of identifying and resolving the resistance profile of microorganisms in fomites present in the hospital environment is highlighted, especially in equipment such as the stethoscope. Emphasizing the use of efficient methods of disinfection, in addition to the use of appropriate therapy for the treatment of these infections.