Mussel-inspired multifunctional surface through promoting osteogenesis and inhibiting osteoclastogenesis to facilitate bone regeneration

ACS Appl. Mater. Interfaces

Shi

Zhu

et al. 2023

Self Cite

Ideal periosteum materials are required to participate in a sequence of bone repair-related physiological events, including the initial immune response, endogenous stem cell recruitment, angiogenesis, and osteogenesis. However, conventional tissue-engineered periosteal materials have difficulty achieving these functions by simply mimicking the periosteum via structural design or by loading exogenous stem cells, cytokines, or growth factors. Herein, we present a novel biomimetic periosteum preparation strategy to comprehensively enhance the bone regeneration effect using functionalized piezoelectric materials. The resulting biomimetic periosteum possessing an excellent piezoelectric effect and improved physicochemical properties was prepared using a biocompatible and biodegradable poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) polymer matrix, antioxidized polydopamine-modified hydroxyapatite (PHA), and barium titanate (PBT), which were further incorporated into the polymer matrix to fabricate a multifunctional piezoelectric periosteum by a simple one-step spin-coating method. The addition of PHA and PBT dramatically enhanced the physicochemical properties and biological functions of the piezoelectric periosteum, resulting in improved surface hydrophilicity and roughness, enhanced mechanical performance, tunable degradation behavior, and stable and desired endogenous electrical stimulations, which is conducive to accelerating bone regeneration. Benefiting from endogenous piezoelectric stimulation and bioactive components, the as-fabricated biomimetic periosteum demonstrated favorable biocompatibility, osteogenic activity, and immunomodulatory functions in vitro, which not only promoted adhesion, proliferation, and spreading as well as osteogenesis of mesenchymal stem cells (MSCs) but also effectively induced M2 macrophage polarization, thereby suppressing reactive oxygen species (ROS)-induced inflammatory reactions. Through in vivo experiments, the biomimetic periosteum with endogenous piezoelectric stimulation synergistically accelerated the formation of new bone in a rat critical-sized cranial defect model. The whole defect was almost completely covered by new bone at 8 weeks post treatment, with a thickness close to that of the host bone. Collectively, with its favorable immunomodulatory and osteogenic properties, the biomimetic periosteum developed here represents a novel method to rapidly regenerate bone tissue using piezoelectric stimulation.

Section: Introductionmentioning

confidence: 99%

Bioinspired Piezoelectric Periosteum to Augment Bone Regeneration via Synergistic Immunomodulation and Osteogenesis

ACS Appl. Mater. Interfaces

Shi

Zhu

et al. 2023

Self Cite

“…Such features endowed the AMAD/MP hydrogel with a strong capacity to promote the transportation of nutrients and metabolic waste, as well as cell infiltration, osteogenesis, and blood vessel in‐growth. [ 29 ] Subsequently, the crystallinity of the AMAD/MP hydrogel was further characterized by XRD. As displayed in Figure 2M, with increasing concentration of MXene@PDA, a sharp (002) peak at 2 θ = 5.8° in the AMAD/MP composite gradually appeared, indicating that MXene@PDA was successfully incorporated with AMAD hydrogels.…”

Section: Resultsmentioning

confidence: 99%

Mild Photothermal‐Stimulation Based on Injectable and Photocurable Hydrogels Orchestrates Immunomodulation and Osteogenesis for High‐Performance Bone Regeneration

Zhu

et al. 2023

Small

Self Cite

A photoactivated bone scaffold integrated with minimally invasive implantation and mild thermal‐stimulation capability shows great promise in the repair and regeneration of irregularly damaged bone tissues. Developing multifunctional photothermal biomaterials that can simultaneously serve as both controllable thermal stimulators and biodegradable engineering scaffolds for integrated immunomodulation, infection therapy, and impaired bone repair remains an enormous challenge. Herein, an injectable and photocurable hydrogel therapeutic platform (AMAD/MP) based on alginate methacrylate, alginate‐graft‐dopamine, and polydopamine (PDA)‐functionalized Ti3C2 MXene (MXene@PDA) nanosheets is rationally designed for near‐infrared (NIR)‐mediated bone regeneration synergistic immunomodulation, osteogenesis, and bacterial elimination. The optimized AMAD/MP hydrogel exhibits favorable biocompatibility, osteogenic activity, and immunomodulatory functions in vitro. The proper immune microenvironment provided by AMAD/MP could further modulate the balance of M1/M2 phenotypes of macrophages, thereby suppressing reactive oxygen species‐induced inflammatory status. Significantly, this multifunctional hydrogel platform with mild thermal stimulation efficiently attenuates local immune reactions and further promotes new bone formation without the addition of exogenous cells, cytokines, or growth factors. This work highlights the potential application of an advanced multifunctional hydrogel providing photoactivated on‐demand thermal cues for bone tissue engineering and regenerative medicine.

“…This may Frontiers in Bioengineering and Biotechnology frontiersin.org be due to the high affinity of copious reactive catechol groups to cells. PDA had been shown to increase the recruitment and proliferation of bone progenitor cells (Hwang et al, 2010;Wu et al, 2022). Allogeneic bone with PDA surface modification promoted bone cell adhesion and proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…( Huang et al, 2022 ). 2) In addition to sticking to VEGF, PDA coating can cling to bone progenitor cells, which promotes bone progenitor cell adhesion and reproduction ( Wu et al, 2022 ; Zhang et al, 2022 ). These factors of this paper are extremely important in clinical application and it is of great significance for clinical healing of bone defects.…”

Section: Discussionmentioning

confidence: 99%

“…Kang et al used a PDA treated titanium surface under alkaline conditions (pH = 8.5) to increase osseointegration and alkaline phosphatase expression in vitro (Kang et al, 2013). Wu et al showed PDA coating to fix a small-molecule activator (LYN-1604), which has excellent osteogenesis-inducing and osteoclastogenesis-inhibiting effects (Wu et al, 2022). Michalicha et al showed a wound dressing with good antibacterial activity was prepared by coupling PDA with antibiotics (Michalicha et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration

Huang

Lü

Front. Bioeng. Biotechnol.

et al. 2022

Objective: Promoting bone regeneration and repairing in bone defects is of great significance in clinical work. Using a simple and effective surface treatment method to enhance the osteogenic ability of existing bone scaffold is a promising method. In this article, we study the application of catecholic amino acid 3,4-dihydroxyphenylalanine (DOPA) surface coating chelated with vascular endothelial growth factor (VEGF) on allogeneic bone.Method: Allogeneic bone is immersed in DOPA solution and DOPA form polydopamine (PDA) with good adhesion. Electron microscopy is used to characterize the surface characteristics of allogeneic bone. MC3T3-E1 cells were tested for biocompatibility and osteogenic signal expression. Finally, a 12-week rabbit bone defect model was established to evaluate bone regeneration capability.Results: We found that the surface microenvironment of DOPA bonded allogeneic bone was similar to the natural allogeneic bone. VEGF loaded allografts exhibited satisfying biocompatibility and promoted the expression of osteogenic related signals in vitro. The VEGF loaded allografts healed the bone defect after 12 weeks of implantation that continuous and intact bone cortex was observed.Conclusion: The PDA coating is a simple surface modification method and has mild properties and high adhesion. Meanwhile, the PDA coating can act on the surface modification of different materials. This study provides an efficient surface modification method for enhancing bone regeneration by PDA coating, which has a high potential for translational clinical applications.