Mussel micronucleus cytome assay

Bolognesi, Claudia; Fenech, Michael

doi:10.1038/nprot.2012.043

Cited by 98 publications

(50 citation statements)

References 87 publications

(137 reference statements)

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“…Micronucleus (MN) frequency was evaluated in hemocyte cells as described elsewhere (Bolognesi and Fenech, 2012). Briefly, a 0.2 mL sample of hemolymph was taken from the posterior adductor muscle and was immediately mixed with 0.2 mL phosphate buffered saline (PBS).…”

Section: Dna Damagementioning

confidence: 99%

The impact of TiO2 nanoparticles on uptake and toxicity of benzo(a)pyrene in the blue mussel (Mytilus edulis)

Farkas

Bergum

Nilsen

et al. 2015

Science of The Total Environment

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Nanoparticles are emerging contaminants of concern. Knowledge on their environmental impacts is scarce, especially on their interactive effects with other contaminants. In this study we investigated effects of titanium dioxide nanoparticles (TiO2NP) on the blue mussel (Mytilus edulis) and determined their influence on the bioavailability and toxicity of benzo(a)pyrene (B(a)P), a carcinogenic polyaromatic hydrocarbon (PAH). Blue mussels were exposed to either TiO2NP (0.2 and 2.0 mg L -1 ) or B(a)P (20 µg L -1 ) and to the respective combinations of these two compounds. Aqueous contaminant concentrations, the uptake of Ti and B(a)P into mussel soft tissue, effects on oxidative stress and chromosomal damage were analyzed. The uncoated TiO2NP agglomerated rapidly in the seawater. The presence of TiO2NP significantly reduced the bioavailability of B(a)P, shown by lowered B(a)P concentrations in exposure tanks and in mussel tissue. The activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were impacted by the various exposures regimes, indicating oxidative stress in the contaminant exposure groups. While SOD activity was increased only in the 0.2TiO2NP exposure group, CAT activity was enhanced in both combined exposure groups.The GPx activity was increased only in the groups exposed to the two single compounds. In hemocytes, increased chromosomal damage was detected in mussels exposed to the single compounds, which was further increased after exposure to the combination of compounds. In this study we show that the presence of TiO2NP in the exposure system reduced B(a)P uptake in blue mussels. However, since most biomarker responses did not decrease despite lower B(a)P uptake in combined exposures, the results suggest that TiO2NP can act as additional stressor, or potentially alters B(a)P toxicity by activation.

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Section: Dna Damagementioning

confidence: 99%

The impact of TiO2 nanoparticles on uptake and toxicity of benzo(a)pyrene in the blue mussel (Mytilus edulis)

Farkas

Bergum

Nilsen

et al. 2015

Science of The Total Environment

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“…Micronuclei were identified according to Bolognesi and Fenech [10]. For each animal 250 cells were counted.…”

Section: Methodsmentioning

confidence: 99%

“…This test is a widely established, relatively easy-to-perform assay. Micronuclei formation indicates chromosomal DNA damage occurring as a result of either chromosome breakage or chromosome mis-segregation during mitosis [10].…”

Section: Introductionmentioning

confidence: 99%

Screening potential genotoxic effect of aquatic plant extracts using the mussel micronucleus test

Eck-Varanka¹,

Kováts²,

Hubai³

et al. 2016

J Coast Life Med

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“…Micronuclei formation indicates chromosomal DNA damage occurring as a result of either chromosome breakage or chromosome mis-segregation during mitosis [1]. In our study, the freshwater bivalve Unio pictorum was selected as test organism.…”

Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

Genotoxic effect ofLythrum salicariaextract determined by the mussel micronucleus test

Eck-Varanka¹,

Kováts²,

Hubai³

et al. 2015

Acta Biologica Hungarica

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A wide range of aquatic plants have been proven to release allelochemicals, of them phenolics and tannin are considered rather widely distributed. Tannins, however, have been demonstrated to have genotoxic capacity. In our study genotoxic potential of Lythrum salicaria L. (Purple Loosestrife, family Lythraceae) was assessed by the mussel micronucleus test, using Unio pictorum. In parallel, total and hydrolysable tannin contents were determined. Results clearly show that the extract had a high hydrolysable tannin content and significant mutagenic effect. As L. salicaria has been long used in traditional medicine for chronic diarrhoea, dysentery, leucorrhoea and blood-spitting, genotoxic potential of the plant should be evaluated not only with regard to potential effects in the aquatic ecosystem, but also assessing its safe use as a medicinal herb.

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Mussel micronucleus cytome assay

Cited by 98 publications

References 87 publications

The impact of TiO2 nanoparticles on uptake and toxicity of benzo(a)pyrene in the blue mussel (Mytilus edulis)

The impact of TiO2 nanoparticles on uptake and toxicity of benzo(a)pyrene in the blue mussel (Mytilus edulis)

Screening potential genotoxic effect of aquatic plant extracts using the mussel micronucleus test

Genotoxic effect ofLythrum salicariaextract determined by the mussel micronucleus test

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