Mutagenesis during plant responses to UVB radiation

Holá, Marcela; Vágnerová, Radka; Angelis, Karel J.

doi:10.1016/j.plaphy.2014.12.013

Cited by 18 publications

(16 citation statements)

References 15 publications

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“…To characterize repair defects of pprad51 , pprtel1 and ppsol we compared their capabilities to remove lesions as DSBs induced by radiomimetic bleomycin, small alkylation adducts induced by methyl methanesulfonate (MMS) and bulky DNA helix distortion by inducing pyrimidine photodimers and 6′‐4′‐photoproducts by UVC irradiation. These lesions represent blocks for DNA replication and are repaired or bypassed by various error‐free as well as error‐prone pathways (Holá et al ., ; Manova and Gruszka, ; Nikitaki et al ., ) The sensitivity of protonemata was tested by 1 h of acute treatment with genotoxin in a liquid medium, followed by explants subculture on Petri plates with the drug‐free medium to determine the ability of the tissue to recover. Explant growth after 3 weeks was monitored as a plant fresh weight rather than plant surface area (Kamisugi et al ., ) as their diameters versus explant mass substantially differed in studied lines (Figure ).…”

Section: Resultsmentioning

confidence: 99%

Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens

et al. 2019

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These authors contributed equally. SUMMARYTelomeres and ribosomal RNA genes (rDNA) are essential for cell survival and particularly sensitive to factors affecting genome stability. Here, we examine the role of RAD51 and its antagonist, RTEL1, in the moss Physcomitrella patens. In corresponding mutants, we analyse their sensitivity to DNA damage, the maintenance of telomeres and rDNA, and repair of double-stranded breaks (DSBs) induced by genotoxins with various modes of action. While the loss of RTEL1 results in rapid telomere shortening, concurrent loss of both RAD51 genes has no effect on telomere lengths. We further demonstrate here the linked arrangement of 5S and 45S rRNA genes in P. patens. The spacer between 5S and 18S rRNA genes, especially the region downstream from the transcription start site, shows conspicuous clustering of sites with a high propensity to form quadruplex (G4) structures. Copy numbers of 5S and 18S rDNA are reduced moderately in the pprtel1 mutant, and significantly in the double pprad51-1-2 mutant, with no progression during subsequent cultivation. While reductions in 45S rDNA copy numbers observed in pprtel1 and pprad51-1-2 plants apply also to 5S rDNA, changes in transcript levels are different for 45S and 5S rRNA, indicating their independent transcription by RNA polymerase I and III, respectively. The loss of SOL (Sog One-Like), a transcription factor regulating numerous genes involved in DSB repair, increases the rate of DSB repair in dividing as well as differentiated tissue, and through deactivation of G2/M cell-cycle checkpoint allows the cell-cycle progression manifested as a phenotype resistant to bleomycin.

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Section: Resultsmentioning

confidence: 99%

Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens

et al. 2019

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“…However, depending on the wavelength, amount of photons, and time of exposure, UV radiation can cause much greater damage, such as mutations, lesions or even plant death (Holá et al, 2015). The strong radiation introduces an amount of energy in the leaf greater than its capacity of use in photosynthesis, overloading the processes, being able to destroy the photosynthetic pigments and the structures of thylakoids (Rai and Agrawal, 2017).…”

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confidence: 99%

How does UV-B radiation affect the initial growth of common bean (Phaseolus vulgaris L.)? Physiological and structural aspects

Wanzeler

Zanetti

Fantinato

et al. 2019

BJD

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ABCTRACTIncreased UV-B radiation, a topic of concern due to climate change, can affect plant development through structural and physiological changes. Common bean (Phaseolus vulgaris L.) is a legume of great economic relevance, being one of the basic components of the human population's diet. Seeds of P. vulgaris var. Carioca were sown and grown in a growth chamber, and at 12 days of age were exposed for 15 min daily to UV-B radiation for a period of 14 days. Growth, leaf anatomy, gas exchange and pigments were evaluated. It was observed that UV-B radiation negatively affected the initial growth of bean plants, which had a reduction in height, number of leaves, fresh and dry leaf masses, leaf area, as well as reduction of stem and root dry masses. There was a reduction in the epidermis thickness, increase in the thickness of the palisade parenchyma and the leaf blade. It was verified yet reduction of the photosynthetic and photoprotectors pigments, reduction of the photosynthetic rate and alterations on the absorbent compounds of the UV-B radiation. The results obtained showed that the increase in UV-B radiation was harmful to the initial development of this crop.Palavras-chave: Agriculture, Anatomy, Beans, Pigments. RESUMOO aumento da radiação UV-B, tema preocupante em virtude das mudanças climáticas, pode afetar o desenvolvimento das plantas, por meio de mudanças estruturais e fisiológicas. O feijoeiro-comum (Phaseolus vulgaris L.) é uma leguminosa de grande relevância econômica, sendo um dos componentes básicos da alimentação humana. Sementes de P. vulgaris var. Carioca foram germinadas e cultivadas em uma câmara de crescimento, sendo aos 12 dias de idade expostas a 15 min diários de radiação UV-B, por um período de 14 dias. Foram avaliados crescimento, anatomia foliar, trocas gasosas e pigmentos. Observou-se que a radiação UV-B afetou negativamente o crescimento inicial do feijoeiro, o qual teve redução em altura, número de folhas, massa fresca, massa seca e área foliares, bem como, redução da massa seca de caule e raiz. Houve redução na espessura da epiderme e aumento nas espessuras do parênquima paliçádico e do limbo. Verificou-se, ainda, redução dos teores de pigmentos fotossintéticos e fotoprotetores, redução da taxa fotossintética e alterações nos compostos absorventes da radiação UV-B. Os resultados obtidos mostraram que o aumento da radiação UV-B foi prejudicial para o desenvolvimento inicial desta cultura.

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“…However, it remains to be elucidated whether such reduced CPD amounts are really due to the activation of dark DNA repair pathway or rather represent dimer dilution by DNA replication mediated by translesion DNA polymerases. Indeed, it has recently been demonstrated that the bypass of non‐repaired CPD, dependent solely on the ongoing DNA synthesis, has been very efficient but more error‐prone and thus responsible for a high mutation rate in plants (Holá et al ). Moreover, it was shown that the endoreduplication may also represent an alternative strategy for plants adapting to increased levels of UV‐B radiation (Gegas et al ).…”

Section: Discussionmentioning

confidence: 99%

“…The negative impact of UV radiation on plants is mainly due to the induction of DNA lesions leading to cell cycle arrest and/or programmed cell death, formation of mutations and elevated genomic instability (Ries et al , Jiang et al , Holá et al ). The main UV‐induced DNA lesions are cis‐syn cyclobutane pyrimidine dimers (CPDs) and 6‐4 photoproducts, with 75–90% prevalence of the former, although their relative proportion may depend on the DNA composition and the local chromatin structure (Britt , Pfeifer , Dany et al ).…”

Section: Introductionmentioning

confidence: 99%

Efficient removal of cyclobutane pyrimidine dimers in barley: differential contribution of light‐dependent and dark DNA repair pathways

Manova

Georgieva

Borisov

et al. 2016

Physiologia Plantarum

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Barley stress response to ultraviolet radiation (UV) has been intensively studied at both the physiological and morphological level. However, the ability of barley genome to repair UV-induced lesions at the DNA level is far less characterized. In this study, we have investigated the relative contribution of light-dependent and dark DNA repair pathways for the efficient elimination of cyclobutane pyrimidine dimers (CPDs) from the genomic DNA of barley leaf seedlings. The transcriptional activity of barley CPD photolyase gene in respect to the light-growth conditions and UV-C irradiation of the plants has also been analyzed. Our results show that CPDs induced in the primary barley leaf at frequencies potentially damaging DNA at the single-gene level are removed efficiently and exclusively by photorepair pathway, whereas dark repair is hardly detectable, even at higher CPD frequency. A decrease of initially induced CPDs under dark is observed but only after prolonged incubation, suggesting the activation of light-independent DNA damage repair and/or tolerance mechanisms. The green barley seedlings possess greater capacity for CPD photorepair than the etiolated ones, with efficiency of CPD removal dependent on the intensity and quality of recovering light. The higher repair rate of CPDs measured in the green leaves correlates with the higher transcriptional activity of barley CPD photolyase gene. Visible light and UV-C radiation affect differentially the expression of CPD photolyase gene particularly in the etiolated leaves. We propose that the CPD repair potential of barley young seedlings may influence their response to UV-stress.

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Mutagenesis during plant responses to UVB radiation

Cited by 18 publications

References 15 publications

Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens

Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens

How does UV-B radiation affect the initial growth of common bean (Phaseolus vulgaris L.)? Physiological and structural aspects

Efficient removal of cyclobutane pyrimidine dimers in barley: differential contribution of light‐dependent and dark DNA repair pathways

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