Mutagenesis in cultured mammalian cells I. Spontaneous gene mutations in human and Chinese hamster cells

Shapiro, N.I.; Khalizev, A.E.; Luss, Eugeny V.; Marshak, Marina I.; Петрова, О. Н.; Varshaver, Nina B.

doi:10.1016/0027-5107(72)90034-6

Cited by 37 publications

(10 citation statements)

References 21 publications

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“…3b`. 8-azg70r-B dose up (Fig. 4b), described induced by radiations and chemical mutagens is affected by such factors as the mutation expression time, the size of the cell inoculum and the concentration of the selective agent (Chu and Malling 1963;Bridges and Huckle 1970;Arlett and Harcourt 1972;Shapiro et al 1972;Duncan and Brookes 1973) . In this experiment, we chose 1 x 105 cells per 90-mm petri dish as the inoculum size and 48 hours as the mutation expression time following the irradiation with X-rays or UV, to standardize the experimental conditions.…”

Section: Resultsmentioning

confidence: 99%

Studies on Somatic Cell Mutations

Horikawa

Suzuki

Ban

1976

The Japanese journal of genetics

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It is very importantto establish the sensitive assay systems for the mutations using mammalian cells in vitro for detecting various potential mutagens present in the human environment.These systems are also useful for studying the mechanisms of somatic mutation (involving the problems of phenotypic expression, repair of premutational damage, specific stage in the cell cycle and so on) in cultured mammalian cells. We found in the previous study (Ban et al. 1976) that the forward mutation system using prototrophic CH-hai C123 cells isolated from an original Chinese hamster hai cell line is much more sensitive than the reverse mutation system using auxotrophic CH-hai Cl 3 cells for detecting the mutations induced by radiations and chemical carcinogens or mutagens.In the present study, as a series of these experiments, we examined the frequencies of the forward and reverse mutations induced by X-rays and UV, using 8-azaguanine (8AG)-sensitive cells (the prototrophic CH-hai C123 cells) and 8AG-resistan cells which are isolated from them, and compared the sensitivities as a mutation assay system with those of the nutritionally forward and reverse mutations used previously (Ban et al. 1976) . Contrary to expectation, the results obtained in this study were rather negative as far as mutation induction are concerned. MATERIALS AND METHODSCells and medium. The origin, culture procedures and properties of the Chinese hamster hai cell line used in the present study have been described previously (Suzuki and Horikawa 1973). Alanine-, asparagine-, proline-, aspartic acid-, hypoxanthine-, and glutamic acid-prototrophic CH-hai Cl 23 cells (Suzuki and Horikawa 1975;Ban et al. 1976) isolated from the cultures of Chinese hamster hai cells by a replica plating method were used as 8AG-sensitive cells. In this study, however, CH-hai C123 cells were cultured in a medium composed of 90% Eagle's MEM+N17 medium (which lacked 1) Present address:

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Section: Resultsmentioning

confidence: 99%

Studies on Somatic Cell Mutations

Horikawa

Suzuki

Ban

1976

The Japanese journal of genetics

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“…On the other hand, the maximum yields of mutations to 6MP-resistance induced by UV-light and to glucose-independence induced by N nitrosomethyl-urea were recorded when cells were transferred to selective media immediately after treatment (Shapiro et al 1972).…”

Section: Discussionmentioning

confidence: 99%

“…It has been found that the following factors influence the mutation of Chinese hamster cells by chemicals and X-rays: the inoculum size, the period between mutagen quenching and addition of a selective agent (mutation expression time), and the concentration of the selective agent (Chu and Malling 1968;Shapiro et al 1972;Duncan and Brookes 1973).…”

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confidence: 99%

Mutagenesis in Cultured Human Diploid Cells

Kuroda

1974

The Japanese journal of genetics

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“…Cultures of skin fibroblasts that are usable for mutation studies can Life span is still an ineluctable limitation, which just hasn't prevented us and others (5,6) from making progress in the study of mutation. The number of doublings allotted to fibroblasts in vitro is usually estimated at 50-60.…”

Section: Human Cells For Mutation Studiesmentioning

confidence: 99%

Mutation studies with human fibroblasts.

DeMars¹

1973

Environ Health Perspect

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Mutagenesis in cultured mammalian cells I. Spontaneous gene mutations in human and Chinese hamster cells

Cited by 37 publications

References 21 publications

Studies on Somatic Cell Mutations

Studies on Somatic Cell Mutations

Mutagenesis in Cultured Human Diploid Cells

Mutation studies with human fibroblasts.

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