Mutagenesis in Escherichia coli IV. Photosensitization to near ultraviolet light by 8-methoxypsoralen

Igali, S.; Bridges, B.A.; Ashwood-Smith, M.J.; Scott, Barry R.

doi:10.1016/0027-5107(70)90067-9

Cited by 139 publications

(23 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As reported by Igali et al in 1970 [12] and Igali and von Borstel in 1974 [i7] the spectrum of mutational events is quite different, for example suppressor mutations are present in a rauch lower frequency after 8 510t ) plus 365 nm light than after 254 nm UV Nght.…”

Section: Discussionmentioning

confidence: 80%

Structural specificity in the lethal and mutagenic activity of furocoumarins in yeast cells

Averbeck

Prakash²,

Biswas

1975

Radiat Environ Biophys

View full text Add to dashboard Cite

Using monofunctional (Angelicin) and bifunctional furocoumarins (Psoralen and 8 Methoxypsoralen) plus 365 nm light it is shown that both damages, the induced monoadducts and/or crosslinks in DNA, provoke lethal and mutgenic effects in haploid and diploid cells of Saccharomyces cerevisiae. Bifunctional furocoumarins are about 20 times more effective in cell killing than Angelicin. Diploid cells are always more resistant than haploid cells. Dark repair (agar haolding) increases survival. This effect can be at least in part correlated to the release of bound material from DNA in dark repair conditions. Bifunctional psoralens (10 mug/ml) are at least 10-fold more effective in inducing nuclear gene black mutations (his- to HIS+) than Angelicin (10 mug/ml) plus 365 nm light or 254 nm ultraviolet light. In contrast cytoplasmic "petite" (delta-) mutations are about as frequently induced by Angelicin plus 365 nm light as by 254 nm UV light. Bifunctional furocoumarins are less effective. The frequency of cytoplasmic "petite" mutations per survivors decreases during dark repair conditions more efficiently after Angelicin than after Psoralen plus 365 nm light treatment.

show abstract

Section: Discussionmentioning

confidence: 80%

Structural specificity in the lethal and mutagenic activity of furocoumarins in yeast cells

Averbeck

Prakash²,

Biswas

1975

Radiat Environ Biophys

View full text Add to dashboard Cite

show abstract

“…Fig. 1 and as presented in column 10 of Table 2 and columns 5 (-NUV) and 7 (+ NUV) of Support for the hypothesis that UvrABC incision is necessary for psoralen crosslink-induced mutagenesis was given by Igali et al (5), who noted that 8-methoxypsoralen produced tryptophan revertants in Uvr+ but not Uvr-E. coli. Yatagai et al (46) observed mutations in uvrB-cells treated with 8-methoxypsoralen, but none were at crosslinkable sites.…”

Section: Resultsmentioning

confidence: 86%

“…It has been apparent for some time that psoralen/NUV treatments producing both crosslinks and monoadducts induce mutations in E. coli (4)(5)(6)(7)(8). However, it was only recently that crosslinks alone were proven to be mutagenic and not just lethal.…”

mentioning

confidence: 99%

Incision by UvrABC excinuclease is a step in the path to mutagenesis by psoralen crosslinks in Escherichia coli.

Sladek

Melian

Howard-Flanders

1989

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

ABSTRACT4,5',8-Trimethylpsoralen (psoralen) plus near UV light produces interstrand crosslinks and monoadducts in DNA, both ofwhich are mutagenic. InEscherichia coil, crosslinks are incised by UvrABC excinuclease, an event that can lead to homologous recombination and repair. To determine whether UvrABC incision of crosslinks is a step in the path to mutagenesis as well as repair, the effect of DNA homologous to a target gene on a plasmid was determined. pSV2-gpt DNA was treated with psoralen and transformed into a pair of hosts: one was gpt', the other was A(gpt-ac)5. The DNA was extracted and transformed into a tester strain

show abstract

“…Long-term sensitivity of affected skin areas to sunlight may follow. Furanocoumarins may also be mutagenic (Igali et al 1970;Townsend et al 1971;Clarke 1974). Numerous cases of phytophotodermatitis attributable to contact with H. mantegazzianum have been documented by medical practitioners (Miescher and Burckhardt 1937;Jones and Russell 1968;Smellie 1968;Drever and Hunter 1970;Camm et al 1976a;Mitchell and Rock 1978) and many For personal use only.…”

Section: Economic Importance and Environmentalmentioning

confidence: 99%

The Biology of Invasive Alien Plants in Canada. 4. Heracleum mantegazzianum Sommier & Levier

Page

Wall

Darbyshire³

et al. 2006

Can. J. Plant Sci.

View full text Add to dashboard Cite

Heracleum mantegazzianum Sommier & Levier. Can. J. Plant Sci. 86: 569-589. Heracleum mantegazzianum (giant hogweed) is an invasive alien plant of management concern in southern Canada where it has escaped from horticulture and established and spread in natural, ruderal, and agricultural ecosystems. It poses a threat to natural ecosystems and human health, and is also a weed in agricultural and urban areas. It is a member of the Carrot family (Apiaceae) and is closely related to the native species Heracleum maximum Bartram (cow-parsnip). It is a monocarpic perennial, which generally flowers in its 3rd or 4th year. Large size, leaf shape, dark reddish pigments in patches on stems and petioles, and fruit characteristics readily distinguish H. mantegazzianum from other plants in Canada. It is increasingly common in riparian areas, floodplains, and forest edges in or near urban areas in southwestern British Columbia and southern Ontario. Based on herbarium specimens, H. mantegazzianum was first recorded in Ontario in 1949, British Columbia in 1964, Nova Scotia in 1980, Quebec in 1990, and New Brunswick in 2000. The development of dense stands of H. mantegazzianum can also reduce the richness of native plants. Contact with H. mantegazzianum can cause phytophotodermatitis, a serious skin inflammation caused by UV photo-activation of furanocoumarins present in the sap. Control methods include herbicide application, mechanical cutting, and animal grazing, but strategies to address seed dispersal and re-establishment from dormant seed must also be adopted. Widespread establishment in southern Canada suggests that eradication is unlikely. However, range expansion and rapid population growth can be prevented through strategic management including public education. Heracleum mantegazzianum (berce du Caucase) est une espèce exotique envahissante qui préoccupe les producteurs du sud du Canada, car la plante s'est échappée des jardins pour coloniser les écosystèmes naturels, rudéraux et agricoles. En plus d'être une adventice dans les régions agricoles et urbaines, l'espèce s'avère une menace pour les écosystèmes naturels et la santé humaine. Membre de la famille de la carotte (Apiacées), la berce du Caucase est étroitement apparentée à l'espèce indigène Heracleum maximum Bartram (berce laineuse). Cette vivace monocarpienne fleurit habituellement la troisième ou la quatrième année. Il est facile de la distinguer des autres plantes à cause de sa grande taille, de la forme de ses feuilles, des taches rouge foncé sur la tige et les pétioles et des fruits très caractéristiques. On la trouve de plus en plus dans les habitats riverains, les plaines inondables et la lisière des forêts, ou à proximité des zones urbaines dans le sud-ouest de la ColombieBritannique et le sud de l'Ontario. D'après les spécimens conservés dans les herbiers, H. mantegazzianum a été identifiée pour la première fois en 1949 en Ontario, en 1964 en Colombie-Britannique, en 1980 en Nouvelle-Écosse, en 1990 au Québec et en 2000 au Nouveau-Brunswick. Le développem...

show abstract

Mutagenesis in Escherichia coli IV. Photosensitization to near ultraviolet light by 8-methoxypsoralen

Cited by 139 publications

References 16 publications

Structural specificity in the lethal and mutagenic activity of furocoumarins in yeast cells

Structural specificity in the lethal and mutagenic activity of furocoumarins in yeast cells

Incision by UvrABC excinuclease is a step in the path to mutagenesis by psoralen crosslinks in Escherichia coli.

The Biology of Invasive Alien Plants in Canada. 4. Heracleum mantegazzianum Sommier & Levier

Contact Info

Product

Resources

About