Mutagenesis of Cellulomonas biazotea for enhanced production of xylanases

Rajoka, Muhammad Ibrahim; Bashir, Aftab; Malik, Kauser A.

doi:10.1016/s0960-8524(97)00116-8

Cited by 35 publications

(62 citation statements)

References 40 publications

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“…Coral et al (2002) reported the optimum temperature for CMCase activity as 40ºC in Aspergillus niger Z10 strain. Rajoka et al (1998) and Rajoka (2004), however, observed suppressed exoglucanase production at temperatures higher than 30ºC in Cellulomonas biazotea and C. flavigens, respectively. At lower temperature, substrate transport across the cells is suppressed and lower product yields are attained.…”

Section: Discussionmentioning

confidence: 92%

Optimization of fermentation conditions for cellulase production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut

Ray¹

2007

Acta Ichthyol. Piscat.

144

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Section: Discussionmentioning

confidence: 92%

Optimization of fermentation conditions for cellulase production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut

Ray¹

2007

Acta Ichthyol. Piscat.

144

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“…The exposure to 115 kG radiation gave approximately a 3 logrithmic reduction (Rajoka et al 1998). The mutated cells were allowed to express in 50 lg ml )1 aspartate (asp) + 1AE0% deoxyglucose (DG) + 5% maize starch in Vogel's medium to enrich aspartate requiring (asp )1 ) and simultaneously derepressed mutants.…”

Section: Selection Of Mutantsmentioning

confidence: 99%

Kinetics of enhanced ethanol productivity using raw starch hydrolyzing glucoamylase from Aspergillus niger mutant produced in solid state fermentation

Rajoka

Yasmin

Latif

2004

Lett Appl Microbiol

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Aims: The present investigation deals with the effect of raw starch hydrolyzing glucoamylase by a derepressed mutant of Aspergillus niger on enhanced productivity of ethanol from uncooked starch under non-aseptic conditions. Methods and Results: The parental culture of Aspergillus niger was improved using c-ray treatment. One derepressed mutant was isolated after extensive screening and optimization and grown on corn cobs, maize starch, soluble starch and wheat bran solid media moistened with Vogel's salts solution and corn steep liquor. The mutant was 2AE5-fold improved over its parent with respect to enzyme productivity, product yield and specific activity. The enzyme from mutated culture was also improved for enzyme properties and could effectively hydrolyze raw starch without the aid of a-amylase. Starch hydrolyzed with mutant-derived glucoamylase supported higher volumetric and product yields of ethanol than those of parental and other strains. Significance and Impact of the Study: The results of the present study are of commercial value. Ethanol product yield coefficient, and volumetric productivity revealed the hyper-productivity of ethanol from raw starch hydrolyzate obtained with mutant-derived glucoamylase without addition of liquefying a-amylase under non-aseptic conditions.

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“…Treatment effects were compared by the protected least significant difference method (20) and have been presented as two-factor design in the form of probability (p) using MstatC software.…”

Section: Discussionmentioning

confidence: 99%

“…Different amounts (250-1500 µg) of 1-methyl 3-nitro 1-nitroso guanidine (NTG) were transferred to different tubes for different time intervals (0-30 min). After the time interval of 15 min, which gave 3 log kill (8,20), cells were washed to get them free of NTG and allowed to get them expressed to a cell absorbance of 0.9.…”

Section: Induction Of Mutationmentioning

confidence: 99%

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Mutation of Aspergillus oryzae for improved production of 3, 4-dihydroxy phenyl-L-alanine (L-DOPA) from L-tyrosine

Haq

Ali

2006

Braz. J. Microbiol.

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Aspergillus oryzae mutant strain UV-7 was further improved for the production of L-DOPA from L-tyrosine using chemical mutation. Different putative mutant strains of organism were tested for the production of L-DOPA in submerged fermentation. Among these putative mutant strains, mutant designated SI-12 gave maximum production of L-DOPA (300 mg L-DOPA.g -1 cells). The production of L-DOPA from different carbon source solutions (So= 30 g.l -1 ) by mutant culture was investigated at different nitrogen sources, initial pH and temperature values. At optimum pH (pHo= 5.0), and temperature (t=30ºC), 100% sugars were utilized for production and cell mass formation, corresponding to final L-DOPA product yield of 150 mg.g -1 substrate utilized, and maximum volumetric and specific productivities of 125 mg.l , respectively. There was up to 3-fold enhancement in product formation rate. This enhancement is the highest reported in literature. To explain the kinetic mechanism of L-DOPA formation and thermal inactivation of tyrosinase, the thermodynamic parameters were determined with the application of Arrhenius model: activation enthalpy and entropy for product formation, in case of mutant derivative, were 40 k j/mol and 0.076 k j/mol. K for L-DOPA production and 116 k j/mol and 0.590 k j/mol. K for thermal inactivation, respectively. The respective values for product formation were lower while those for product deactivation were higher than the respective values for the parental culture. Therefore, the mutant strain was thermodynamically more resistant to thermal denaturation.

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Mutagenesis of Cellulomonas biazotea for enhanced production of xylanases

Cited by 35 publications

References 40 publications

Optimization of fermentation conditions for cellulase production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut

Optimization of fermentation conditions for cellulase production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut

Kinetics of enhanced ethanol productivity using raw starch hydrolyzing glucoamylase from Aspergillus niger mutant produced in solid state fermentation

Mutation of Aspergillus oryzae for improved production of 3, 4-dihydroxy phenyl-L-alanine (L-DOPA) from L-tyrosine

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