Mutagenic activity of estradiol evaluated by an<i>in vitro</i>micronucleus assay

Djelić, Ninoslav; Spremo-Potparević, Biljana; Djelic, Dijana

doi:10.1556/abiol.56.2005.3-4.22

Cited by 7 publications

(4 citation statements)

References 5 publications

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“…The mutagenic potential of these compounds can be expressed only after its metabolic activation, mainly by the cytochrome P450,30 which can be accentuated by other substances or chemicals (alcohol, caffeine, cocaine, tobacco, drugs, etc) 20…”

Section: Discussionmentioning

confidence: 99%

Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders

et al. 2007

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Objective:To evaluate genotoxicity of anabolic androgenic steroids (AAS) in male bodybuilders by a micronucleus assay in buccal mucosa cells.Methods:11 male bodybuilders volunteered to participate in this study and two groups were formed: group 1 (n = 6), without AAS consumption and group 2 (n = 5), with AAS consumption. A sample of buccal epithelium was taken from each participant once a week for 6 weeks. Samples were fixed, stained and analysed by a light microscope, and 2000 cells were counted from each slide. Results are expressed as micronucleated cells (MNC) per 1000 cells and were analysed by the Mann–Whitney U test and Wilcoxon’s test.Results:A marked increased in MNC was seen in bodybuilders with AAS consumption compared with those without AAS consumption (mean (SD) 4.1 (2.4) MNC/1000 cells vs 0.4 (0.4) MNC/1000 cells, respectively; p<0.004). Intragroup comparisons showed no differences in the MNC frequencies during the sampling time in group 1, whereas the MNC frequency in group 2 varied significantly, reaching the highest MNC frequencies in the third and fourth week of sampling (5.9 (2.4) MNC/1000 cells; 5.8 (1.8) MNC/1000 cells, respectively); frequency in the first sampled week was 1.1 (0.1) MNC/1000 cells. Significant differences in all sampled weeks were found between the two groups.Conclusion:AAS consumption increased the frequency of MNC from buccal mucosa in bodybuilders.

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Section: Discussionmentioning

confidence: 99%

Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders

et al. 2007

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“…This bond can be produced with separate DNA chains, between the bases of the same DNA molecule, or between the DNA and RNA, forming MN during cell division. 30 The collateral effects of alkyl agent treatment include toxicity and cancer due to genetic damage. The mutagenic potential of these compounds can be expressed only after its metabolic activation, mainly by the cytochrome P450, 30 which can be accentuated by other substances or chemicals (alcohol, caffeine, cocaine, tobacco, drugs, etc).…”

Section: Discussionmentioning

confidence: 99%

“…30 The collateral effects of alkyl agent treatment include toxicity and cancer due to genetic damage. The mutagenic potential of these compounds can be expressed only after its metabolic activation, mainly by the cytochrome P450, 30 which can be accentuated by other substances or chemicals (alcohol, caffeine, cocaine, tobacco, drugs, etc). 20 Toxicity, mutagenicity, genotoxicity and cancerogenesis of sexual hormones are the result of a combination of genetic and epigenetic factors.…”

Section: Discussionmentioning

confidence: 99%

Commentary on “Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders”

Vuyyuri¹

2007

Br J Sports Med

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“…In our previous studies we demonstrated that 17b-oestradiol induces sisterchromatid exchanges (Djeli} and Djeli}, 2002) and micronuclei (Djeli} et al, 2005) in cultured human lymphocytes. These results are in accordance with findings of genotoxic effects of natural (Ahmad et al, 2000) and synthetic oestrogens (Hundal et al, 1997)…”

Section: Introductionmentioning

confidence: 90%

Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro

Đelić¹,

Spremo-Potparević²,

Marković³

et al. 2006

Acta vet. (Beogr.)

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Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level

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Mutagenic activity of estradiol evaluated by anin vitromicronucleus assay

Cited by 7 publications

References 5 publications

Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders

Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders

Commentary on “Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders”

Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro

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