Mutagenic Effect of Gamma Radiation on Macro Mutation Spectrums, Effectiveness and Efficiency under M3 Generation in Pea (Pisum sativum L.)

Kumar, Arvind; Chaurasia, A. K.; Yadav, Pramod Kumar

doi:10.9734/jalsi/2020/v23i430157

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…Similar to seedling height, the greater the dosage of gamma radiation, the lower the pollen fertility %. This conclusion was already endorsed by a number of studies [37][38][39][40][41][42]. Kumar, et al [38] demonstrated that when dried seeds of pea were irradiated, a 5 kR dosage of gamma rays induced 10.06 percent pollen sterility, while a 40 kR exposure generated 53.12 percent pollen sterility.…”

Section: Plos Onementioning

confidence: 82%

“…This conclusion was already endorsed by a number of studies [ 37 – 42 ]. Kumar, et al [ 38 ] demonstrated that when dried seeds of pea were irradiated, a 5 kR dosage of gamma rays induced 10.06 percent pollen sterility, while a 40 kR exposure generated 53.12 percent pollen sterility. Additionally, when presoaked seeds were utilized, they observed an increase in pollen sterility.…”

Section: Discussionmentioning

confidence: 99%

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Morphometric frequency and spectrum of gamma-ray-induced chlorophyll mutants identified by phenotype and development of novel variants in lentil (Lens culinaris Medik.)

Pramanik¹,

Debnath²,

Rahimi³

et al. 2023

PLoS ONE

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Genetic variations are a crucial source of germplasm heterogeneity, as they contribute to the development of new traits for plant breeding by offering an allele resource. Gamma rays have been widely used as a physical agent to produce mutations in plants, and their mutagenic effect has attracted much attention. Nonetheless, few studies have examined the whole mutation spectrum in large-scale phenotypic evaluations. To comprehensively investigate the mutagenic effects of gamma irradiation on lentils, biological consequences on the M1 generation and substantial phenotypic screening on the M2 generation were undertaken. Additionally, the study followed the selected mutants into the M3 generation to evaluate the agronomic traits of interest for crop improvement. Seeds of lentil variety Moitree were irradiated with a range of acute gamma irradiation doses (0, 100, 150, 200, 250, 300, and 350 Gy) to induce unique genetic variability. This research focused on determining the GR50 value while considering seedling parameters and examining the status of pollen fertility while comparing the effects of the gamma irradiation dosages. The GR50 value was determined to be 217.2 Gy using the seedling parameters. Pollens from untreated seed-grown plants were approximately 85% fertile, but those treated with the maximum dosage (350 Gy) were approximately 28% fertile. Numerous chlorophyll and morphological mutants were produced in the M2 generation, with the 300 Gy -treated seeds being the most abundant, followed by the 250 Gy -treated seeds. This demonstrated that an appropriate dosage of gamma rays was advantageous when seeking to generate elite germplasm resources for one or multiple traits. Selected mutants in the M3 generation showed improved agronomic traits, including plant height, root length, number of pods per plant, and yield per plant. These investigations will contribute to a comprehensive understanding of the mutagenic effects and actions of gamma rays, providing a basis for the selection and design of suitable mutagens. This will facilitate the development of more controlled mutagenesis protocols for plant breeding and help guide future research directions for crop improvement using radiation-induced mutation breeding techniques.

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Section: Plos Onementioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Morphometric frequency and spectrum of gamma-ray-induced chlorophyll mutants identified by phenotype and development of novel variants in lentil (Lens culinaris Medik.)

Pramanik¹,

Debnath²,

Rahimi³

et al. 2023

PLoS ONE

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Biological effect of gamma and electron beam to target turning X-ray irradiation on two varieties of freesia in M3 generation

Xu,

Wang,

Matina

et al. 2023

Journal of Radiation Research and Applied Sciences

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Effect of different doses of chemical and physical mutagen on genetic variability and character association in M1 and M2 generation of Momordical balsamina L.

Bammanakatti¹,

Rathod²,

Gasti³

et al. 2023

Preprint

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Momordica balsamina L. commonly known as the Balsam apple is an under-exploited cucurbit vegetable occurs wild type in nature which offers various dietary and nutraceutical properties. It is an annual to perennial, tendril-bearing herb, native to tropical regions of Africa. Since the crop occurs in wild forms, the variation exists among the genotype is limited. For the improvement of plant characteristics in diverse crops, mutation breeding has been extensively applied. In the present study, treated population was evaluated to study the extent of genetic variability and character association on growth and yield parameters in M1 and M2 generation of Momordica balsamina L. The results revealed that high heritability was exhibited by vine length at harvest, number of branches per plant at harvest, stem diameter, leaf area, number of fruits per plant, fruit girth, lycopene content, β-carotene content and ascorbic acid content. As per the character association studies, fruit yield per plant showed highly significant and positive association with number of fruits per plant, fruit weight, fruit length and days to first flowering. Whereas, highly significant but negative association was revealed by number of branches per plant at harvest, stem diameter and days to 50 per cent flowering among mutant population. Whereas, vine length at harvest, number of fruits per plant, fruit weight, fruit length and fruit girth recorded highest positive direct effect on fruit yield per plant. While, number of branches per plant at harvest, stem diameter and days to 50 per cent flowering exerted negative direct effect on fruit yield per plant.

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Mutagenic Effect of Gamma Radiation on Macro Mutation Spectrums, Effectiveness and Efficiency under M3 Generation in Pea (Pisum sativum L.)

Cited by 3 publications

References 3 publications

Morphometric frequency and spectrum of gamma-ray-induced chlorophyll mutants identified by phenotype and development of novel variants in lentil (Lens culinaris Medik.)

Morphometric frequency and spectrum of gamma-ray-induced chlorophyll mutants identified by phenotype and development of novel variants in lentil (Lens culinaris Medik.)

Biological effect of gamma and electron beam to target turning X-ray irradiation on two varieties of freesia in M3 generation

Effect of different doses of chemical and physical mutagen on genetic variability and character association in M1 and M2 generation of Momordical balsamina L.

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