Mutagenic effectiveness and efficiency of gamma rays and combinations with EMS in the induction of macro mutations in blackgram (Vigna mungo (L.) Hepper)

Tamilzharasi, M.; Dharmalingam, Kumaresan; Venkatesan, T.; Souframanien, J.; Jayamani, Palaniappan

doi:10.1016/j.apradiso.2022.110382

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…Charivnytsya. In our hands EMS had a lower mutation frequency than NMU or DG-2, but it has been shown that EMS in combination with X-ray treatment (Datta and Biswas, 1985, using N. damascena) or gamma-radiation (Gosh and Datta, 2005, using N. damascena;Kaul andBahn, 1977, using rice andTamilzharasi et al, 2022, using black mung bean) can enhance mutation frequency (Datta and Biswas, 1985). This could be a direction of our future research.…”

Section: Discussionmentioning

confidence: 62%

Mutation types and frequency in Nigella damascena L. in the М2 and M3 generation, using ethyl methanesulfonate, nitrosomethylurea and a new derivative of dimethylsulfate, DG-2

Gubanova

2023

Agric. sci. pract.

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Aim. To identify mutations and evaluate the mutation frequency in Nigella damascena L. cultivars (cvs) Bereginya and Charivnytsya (M2 and M3 generation), following treatment of their seeds with ethyl methanesulfonate (EMS), nitrosomethylurea (NMU) and a new derivative of dimethyl sulfate, DG-2. Methods. Treated Nigella seeds of two cvs with the mutagens for 6 and 16 h and in concentrations of 0.01 and 0.5 % for EMS and NMU and 0.05 and 0.5 % for DG-2. Results. A wide range of mutations (59 types) was obtained, that was divided into six groups: five groups with changes in the morphological type and one group with changes in the physiological type. Among the detected mutations, there were both previously known mutations and those obtained in this culture for the first time. The high- est mutation frequency (30 %) affecting synthesis of chlorophyll and structure of stem, shoots and leaves and 20 % for physiological features, was registered for NMU at 16 h and 0.05 % in cv. Bereginya. However, this NMU concentra- tion appeared to be lethal for cv. Charivnytsya. Conclusions. The new mutagen DG-2 proved to be most effective for inducing mutations in the corolla petal color of nigella, namely 4.0 at a 0.5 % concentration of the mutagen and 16h exposure for cv. Bereginya and 4.0 % at the same concentration and exposure for cv. Charivnytsya. DG-2 caused a substantial number of mutations in all six mutation groups affecting morphological and physiological traits. The clas- sic mutagen EMS was also effective across the spectrum of mutation groups in our study; however, it caused mutations at a lower frequency. The maximum mutation frequency under influence of EMC at a concentration of 0.05 % and an exposure of 16 h in cv. Bereginya was 11.0 %, and in cv. Charivnytsya 8.0 %. For all three mutagens used, an increase in the concentration of the active substance and of exposure time led to an increase in the mutation frequency in N. damascena plants. We will select mutants with economically valuable traits, such as tall, lodging-resistant plants and early maturing ones, for further work on the development of new cultivars of N. damascena for industrial cultivation.

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Section: Discussionmentioning

confidence: 62%

Mutation types and frequency in Nigella damascena L. in the М2 and M3 generation, using ethyl methanesulfonate, nitrosomethylurea and a new derivative of dimethylsulfate, DG-2

Gubanova

2023

Agric. sci. pract.

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“…Although there are many ways by which one or more traits were enhanced in crops to increase crop yield and quality, the efficiency among these methods can be drastically different ( Saker and Singh, 2015 ; Khosa et al., 2016 ). Mutations introduced via physical, chemical, or biotechnological methods are useful tools in crop breeding ( Mahadtanapuk et al., 2014 ; Jung and Till, 2021 ; Tamilzharasi et al., 2022 ). In order to increase seed oleic acid content, previous investigations indicate two methods that have been extensively applied, which are chemical mutation (EMS) and biological mutation ( Auld et al., 1992 ; Sivaraman et al., 2004 ; Peng et al., 2010 ; Lee et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-mediated editing of double loci of BnFAD2 increased the seed oleic acid content of rapeseed (Brassica napus L.)

et al. 2022

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Seed oleic acid is an important quality trait sought in rapeseed breeding programs. Many methods exist to increase seed oleic acid content, such as the CRISPR/Cas9-mediated genome editing system, yet there is no report on seed oleic acid content improvement via this system’s precise editing of the double loci of BnFAD2. Here, a precise CRISPR/Cas9-mediated genome editing of the encoded double loci (A5 and C5) of BnFAD2 was established. The results demonstrated high efficiency of regeneration and transformation, with the rapeseed genotype screened in ratios of 20.18% and 85.46%, respectively. The total editing efficiency was 64.35%, whereas the single locus- and double locus-edited ratios were 21.58% and 78.42%, respectively. The relative proportion of oleic acid with other fatty acids in seed oil of mutants was significantly higher for those that underwent the editing on A5 copy than that on C5 copy, but it was still less than 80%. For double locus-edited mutants, their relative proportion of oleic acid was more than 85% in the T1 and T4 generations. A comparison of the sequences between the double locus-edited mutants and reference showed that no transgenic border sequences were detected from the transformed vector. Analysis of the BnFAD2 sequence on A5 and C5 at the mutated locus of double loci mutants uncovered evidence for base deletion and insertion, and combination. Further, no editing issue of FAD2 on the copy of A1 was detected on the three targeted editing regions. Seed yield, yield component, oil content, and relative proportion of oleic acid between one selected double loci-edited mutant and wild type were also compared. These results showed that although the number of siliques per plant of the wild type was significantly higher than those of the mutant, the differences in seed yield and oil content were not significant between them, albeit with the mutant having a markedly higher relative proportion of oleic acid. Altogether, our results confirmed that the established CRISPR/Cas9-mediated genome editing of double loci (A5 and C5) of the BnFAD2 can precisely edit the targeted genes, thereby enhancing the seed oleic acid content to a far greater extent than can a single locus-editing system.

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“…These mutants with increased number of parts and about size were found to be correlated with plant yield and hence subsequent research has to be carried out with several clinical parameters. Tamilzharasi et al (2019a), Tamilzharasi et al (2022c) observed various mutation frequencies and span with different concentration of EMS in the M2 generation of black gram. The study Tamilzharasi et al (2021b) founded a significant correlation between the biological damage in M1 generation with the information provided in the studies the mutagenic sensitivity can be understand on the surviving plants (Goyal et al, 2020, Serrat et al, 2014, Ramya et al, 2014.…”

Section: Introductionmentioning

confidence: 99%

Assessment on Seedling Traits of Black Gram (Vigna mungo L. Hepper) Mutants Raised during M2 Generation

Nayak,

Naik,

Padiya

et al. 2023

IJEP

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The present study was conducted at department of Genetics, Osmania University, Hyderabad, Telangana, India, during September to November, 2021 with the blackgram accession- IC-436524 obtained from NBPGR regional centre, Hyderabad, Telangana, India and the check T9 was collected from ICAR-CRIDA Hyderabad. The present study aimed to estimate the mutagenic sensitivity of black gram accession- IC-436524 with Ethyl methane sulfonate. Accordingly, this study checked whether the mutagenic activity has been inherited to the next generation M2. Data analysis has been performed for the determination of significance in germination percentage, seedling length (cm), seedling vigor index, root length (cm) and seed survival rate (Day 1 to Day 15) that were observed to be highly significant (p<0.01) for above characters except root length (cm) for treatments. It clearly indicated that there was adequate amount of variability among the black gram mutant due to treatments. The collected data for 7th day observed that seed germination percentage was higher at 0.2% and 0.3% EMS treated mutants and lowest germination percentage was observed in 0.5% EMS treated mutants. Seedling length (cm) was recorded highest in 0.2% EMS treated mutants and lowest seedling length (cm) was recorded in control. Maximum seedling vigor index was recorded in 0.2% EMS treated mutants and lowest seedling vigor index was showed for 0.5% EMS treated mutants. Seedling survival rate data was recorded for 15th day showed higher in 0.2% EMS treated mutants. Data showed EMS has induced mutations during M1 generation and inherited to offspring (M2 generation).

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Mutagenic effectiveness and efficiency of gamma rays and combinations with EMS in the induction of macro mutations in blackgram (Vigna mungo (L.) Hepper)

Cited by 5 publications

References 12 publications

Mutation types and frequency in Nigella damascena L. in the М2 and M3 generation, using ethyl methanesulfonate, nitrosomethylurea and a new derivative of dimethylsulfate, DG-2

Mutation types and frequency in Nigella damascena L. in the М2 and M3 generation, using ethyl methanesulfonate, nitrosomethylurea and a new derivative of dimethylsulfate, DG-2

CRISPR/Cas9-mediated editing of double loci of BnFAD2 increased the seed oleic acid content of rapeseed (Brassica napus L.)

Assessment on Seedling Traits of Black Gram (Vigna mungo L. Hepper) Mutants Raised during M2 Generation

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