Mutagenic effectiveness and efficiency of Gamma rays and EMS in Lablab purpureus (L.) Sweet var. typicus

Abstract

Mutation has been successfully employed in breeding of several food crop varieties, ornamentals and export crops. The aim of this study was undertaken to assess the effectiveness and efficiency of EMS and gamma rays on Lablab purpureus (L.) Sweet var. typicus cv. CO(Gb)14. Genetically pure, uniform and dry seeds were treated with different doses/ concentrations of gamma rays (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 KR) and EMS (5mM, 10mM, 15mM, 20mM, 25mM, 30mM, 35mM, 40mM, 45mM and 50mM). Mutagenic effectiveness and efficiency of gamma rays and EMS was calculated on the biological damage (lethality, injury and pollen sterility) in the M 1 generation and both chlorophyll and morphological mutations observed in the M 2 generation. The results indicated that, mutagenic effectiveness and efficiency decreased with increase in mutagenic treatments.

Assessment of Bio-physiological damages and cytological aberrations in cowpea varieties treated with gamma rays and sodium azide

“…As expected, we noticed a progressive decline in the pollen fertility percentage with the increase in the mutagen doses. The results of the present study were in propinquity with the findings of previous studies in various crops such as garden beans [ 37 ], lentils [ 24 ], [ 38 ], horse gram [ 39 ], and pigeon pea [ 40 ]. The normal segregations of chromosomes without any structural alterations are important in developing normal pollen grains.…”

Effectiveness and Efficiency of Different Doses of Physical Mutagen (γ- rays) in Clusterbean [Cyamopsis tetragonoloba (L.) Taub.] Genotypes

“…The results of present investigations are in conformity with these results. Similar results were also obtained by More and Borkar (2016) in French bean, Monica and Seetharaman (2016) in lablab.…”

Effect of gamma irradiation on the germination, pollen viability, and morpho-agronomic of Pachyrhizus erosus cv. Kota Padang

“…Generally, the effect of primary injury on mutant growth and yield was a reduction in growth and fertility, which finally led to lethality (Kodym et al 2011). The effect of irradiation mutation on low pollen fertility percentage was due to meiotic aberration resulting in abnormal pollen grains formation (Mathusamy and Jayabalan 2002;Monica and Seetharaman 2015). Reduction in pollen fertility due to irradiation and chemical mutation was reported earlier in cowpea (Bin and Dwivedi 2014), pigeon pea (Sangle et al 2011), and garden bean (Monica and Seetharaman 2015).…”

“…Anther was then extracted on glass slides, and 1% KI was dropped into pollen grains and incubated for 2 hours before observation was conducted using the microscope. A fully stained grain reflected fertile, while partial stained, shrunken and empty reflected sterile pollen grains (Monica and Seetharaman 2015). The viability of pollen by KI test was found out as formulae below.…”

“…In the study, the effect of gamma-rays on pollen viability was also observed. Pollen viability is one of the essential stable and genetically controlled traits (Monica and Seetharaman 2015). Data presented in Table 2 represents the direct effects of irradiation mutation on pollen viability.…”