Mutagenic effects of chromium trioxide on root tip cells of Vicia faba

Xiaowei, Qian

doi:10.1631/jzus.2004.1570

Cited by 52 publications

(28 citation statements)

References 3 publications

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“…The variation of chromosomal aberrations with the different test liquids was however not dose-dependent. This is in agreement with Odeigah et al (1997) but in contrast to the observations by Qian (2004) who reported that chromosome aberration increased with increasing effluent concentration. A possible explanation for the former is that with increasing concentration, and consequently increasing toxicity, there was an inhibitory effect on cell division.…”

Section: Microscopic Effectssupporting

confidence: 91%

Genotoxicity assessment of three industrial effluents using the Allium cepa bioassay

Ibeh¹,

Umeham²

2018

Afr. J. Environ. Sci. Technol.

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The Allium cepa assay was employed, in conjunction with physico-chemical analysis, to investigate the potential cytotoxicity and genotoxicity of three industrial effluents (soap, beverage and paint) from the southeast of Nigeria. For in situ monitoring of cytotoxicity level, inhibition of mitotic division was investigated and for genotoxicity evaluation, chromosomal aberration assay was carried out. The results showed certain sample-constituents of the wastewaters (e.g. pH, turbidity) to be at concentrations beyond the maximum permissible limits required by international regulatory authorities. On the basis of the 72 h effective concentration (72 h EC 50 ), the paint effluent was the most toxic while the beverage effluent was the least toxic. The mean root lengths of A. cepa exposed to different concentrations of the industrial effluents, when compared to the control, were shown by Analysis of Variance (ANOVA) to be significantly (p<0.05) concentration dependent. The three industrial effluents were observed to induce chromosomal aberrations, laggards and sticky chromosomes being the most frequently seen. The findings show that a combination of physico-chemical analysis and genotoxicity assay is effective in assessing industrial effluents for the environmental monitoring of pollutants.

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Section: Microscopic Effectssupporting

confidence: 91%

Genotoxicity assessment of three industrial effluents using the Allium cepa bioassay

Ibeh¹,

Umeham²

2018

Afr. J. Environ. Sci. Technol.

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“…Laggard chromosomes depend upon the moving speed and process of an individual chromosome differing from normal ones (Qian 2004). Rivetta et al (1997), found that Cd binds to CAM calmodulin and competes with Cd in these bindings.…”

Section: Resultsmentioning

confidence: 99%

Comparative genotoxicity of heavy metals in root meristems of <i>Cuminum cyminum</i> L.

Kumar¹,

Bhardwaj²

2017

Chromosome Botany

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Effects of heavy metals are very extreme on biological organisms. Pollution causes accumulation of metals in soil which adversely affects the yield attributes of the crop plants. Cuminum cyminum L., (Cumin) is a major spice growing crop of India. The present study has been focused to scrutinize the cytogenetic effects of Chromium (Cr), Cadmium (Cd) and Lead (Pb) on root meristems of Cuminum cyminum L. During experiment, germinated seeds of cumin were exposed to four different concentrations viz., 50ppm, 100ppm, 150ppm and 200ppm of metallic salts of Chromium (Cr 2 O 3 ), Cadmium(CdCl 2 ) and Lead [Pb(NO 3 ) 2 ], respectively. The AMI% (Active Mitotic Index) was observed to show a sharp decline whereas TAB% (Total abnormality percentage) was elevated with increasing metal concentrations. Various Chromosomal aberrations were recorded viz., scattering, stickiness, precocious movement, laggards, bridges, c-metaphase, etc. Among the various types of chromosomal aberrations encountered, stickiness was dominant among all. On comparison Pb was found to be more genotoxic followed by Cd and Cr, respectively.

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“…Then they were stored in 70% ethanol at a temperature of +4 C. After they were hydrolyzed in 1 N HCl at a temperature of 60 C for 20 min, they were treated with 45% acetic acid solution for 30 min, and stained with acetocarmine for 24 h. In the last stage, the root tips were crushed in 45% acetic acid and photographed at 500 zoom with a binocular research microscope (Qian 2004, Staykova et al 2005.…”

Section: Micronucleus Assay and Chromosomal Aberrationsmentioning

confidence: 99%

The Investigation of Genotoxic, Physiological and Anatomical Effects of Paraquat Herbicide on <i>Allium cepa</i> L.

et al. 2015

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SummaryThe aim of this study was to investigate the toxic effects of different doses of Paraquat treatment on Allium cepa L. Root length (RL), germination percentage (GP), weight gain (WG), chromosomal aberrations (CAs), micronucleus (MN) frequency and anatomical alterations (AA) were used as toxicity indicators, and the data were correlated with statistical parameters. The seeds were seperated into four groups; one as control and three as Paraquat treatment groups. Paraquat doses of 10, 50, and 100 ppm were used in treatment groups during 72 h. The results indicated that Paraquat showed dose-dependent effects in the RL, GP, WG, CAs, MN frequency and AA on Allium cepa L. seeds. Increasing the dose of Paraquat significantly decreased RL, WG and GP in all of the treatment groups. The highest rate for RL, GP and WG were observed in the control group. Also, the frequency of MN and CAs showed dose-dependent increase in all of the Paraquat treatment groups. The rate of MN formation and CAs frequency were observed to be highest at the 100-ppm dose of Paraquat and lowest at the 10-ppm dose of Paraquat. Paraquat treatment caused anatomical alterations on root tips and rate of anatomical alterations increased with increasing dose of Paraquat. The results of this study showed that the widely used Paraquat herbicide showed toxic effects at certain concentrations which was demonstrated using Allium cepa L. test material. The selected parameters such as RL, GP, WG, CAs, MN frequency and AA are very sensitive and useful biomarkers for biomonitoring these effects.

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Mutagenic effects of chromium trioxide on root tip cells of Vicia faba

Cited by 52 publications

References 3 publications

Genotoxicity assessment of three industrial effluents using the Allium cepa bioassay

Genotoxicity assessment of three industrial effluents using the Allium cepa bioassay

Comparative genotoxicity of heavy metals in root meristems of <i>Cuminum cyminum</i> L.

The Investigation of Genotoxic, Physiological and Anatomical Effects of Paraquat Herbicide on <i>Allium cepa</i> L.

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