Mutagenic Effects of Gamma-rays and EMS on M1 Population of Rice Bean (Vigna umbellata Thunb, Ohwi &amp; Ohashi)

Mishra, Dayanidhi; Bhoi, Lopamudra; Tripathy, Swapan K.; Behera, Mohima Prasad; Mishra, Tapas

doi:10.20546/ijcmas.2018.709.421

Cited by 3 publications

(3 citation statements)

References 4 publications

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“…Elimination of damaged zones by inhibition of cell division at an earlier stage and replacement of injured meristematic cells may result in the reduction of plant height at maturity and Kadambavanasundaram, 1973). The extreme dwarf plants were induced by combination treatment of 60 Co gamma + EMS which confirmed with the reports of Mishra et al (2018) in rice bean.…”

Section: Number Of M1 Families Segregating In M2 Generation *supporting

confidence: 88%

Assessment of mutagenic sensitivity in blackgram variety CO 6

Tamilzharasi

Kumaresan²,

Venkatesan³

et al. 2021

EJPB

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The present study was conducted to examine the mutagenic effect of 60 Co gamma rays and a combination of 60 Co gamma rays with EMS in blackgram variety CO 6. Based on the LD 50 dose, the seeds were treated with three different doses of gamma rays (200, 300, 400 Gy) and recurrent doses of 20 mM and 30 mM EMS were treated with gamma ray irradiated seeds for combination treatments. The M 1 generation was evaluated in the field for the following parameters viz., germination percentage, plant survival percentage, plant height at 30 DAS and maturity, pollen fertility and seed fertility. A dose-dependent decrease was noticed for all the characters under this study in M 1 generation. Overall, the maximum reduction was noticed at a higher dose of 400 Gy gamma rays and 400 Gy + 30 mM EMS mutagenic treatments. Among the mutagenic treatments, a combination of 60 Co gamma ray + EMS exhibited the maximum effect with a higher percentage of reduction in all the traits while increased pollen sterility was found to be associated with a corresponding increase in dose/concentration of mutagenic agents. A significant positive correlation was observed between viable mutation frequency and biological damage caused by mutagens in M 1 generation. Hence, the higher doses of 60 Co gamma rays (300 and 400 Gy) and 60 Co gamma rays +EMS (300 Gy+ 30; 400 Gy+ 20 and 400 Gy+ 30 mM) proved to be efficient in increasing the mutation frequency towards desirable directions.

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Section: Number Of M1 Families Segregating In M2 Generation *supporting

confidence: 88%

Assessment of mutagenic sensitivity in blackgram variety CO 6

Tamilzharasi

Kumaresan²,

Venkatesan³

et al. 2021

EJPB

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“…The extent of mutations introduced by a chemical mutagen depends on the treatment procedure, even if the same agent is used. We used a conventional method for EMS mutagenesis, and the frequency of albinos in our study (Table 1) was within the frequency range (0.0010–18%) obtained in other studies of EMS‐mutagenized rice (Mishra et al., 2019; Mohapatra et al., 2014; Rajarajan et al., 2014; Rao, 1977; Wu et al., 2005). The density detected by genome resequencing was 2.3–14.7 mutations per Mbp in our study (Table 2) and 0.2–10 mutations per Mbp in previous studies (see a review Viana et al., 2019).…”

Section: Discussionsupporting

confidence: 75%

M₂ plants derived from different tillers of a chemically mutagenized rice M₁ plant carry independent sets of mutations

2023

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SUMMARYGeneration of mutant populations with high genetic diversity is key for mutant screening and crop breeding. For this purpose, the single‐seed descent method, in which one mutant line is established from a single mutagenized seed, is commonly used. This method ensures the independence of the mutant lines, but the size of the mutant population is limited because it is no greater than the number of fertile M1 plants. The rice mutant population size can be increased if a single mutagenized plant produces genetically independent siblings. Here, we used whole‐genome resequencing to examine the inheritance of mutations from a single ethyl methanesulfonate (EMS)‐mutagenized seed (M1) of Oryza sativa in its progeny (M2). We selected five tillers from each of three M1 plants. A single M2 seed was selected from each tiller, and the distributions of mutations induced by EMS were compared. Surprisingly, in most pairwise combinations of M2 siblings from the same parent, ≥85.2–97.9% of all mutations detected were not shared between the siblings. This high percentage suggests that the M2 siblings were derived from different cells of the M1 embryo and indicates that several genetically independent lines can be obtained from a single M1 plant. This approach should allow a large reduction in the number of M0 seeds needed to obtain a mutant population of a certain size in rice. Our study also suggests that multiple tillers of a rice plant originate from different cells of the embryo.

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“…The initial stages of gamma-ray irradiation affected more on quantitative plant characteristics, which lead to morphological abnormalities like plant height and panicle length [13]. Gamma-ray irradiation causes plants to repair themselves due to chromosome damage leading to inhibition of plant growth [14]. Decreasing of panicle length resulting in fewer grains formed on the plants.…”

Section: Phenotype Of Black Ricementioning

confidence: 99%

Selection of M3 mutant strains from 200 gy gamma irradiation on Cempo Ireng

Nandariyah,

Sukaya,

Sutarno

et al. 2023

E3S Web Conf.

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Climate change is a serious issue that can disrupt crop production and pest resistance, especially in rice crops. To tackle climate change, plant breeding can be employed to develop new characteristics that are resistant, using physical mutagenesis like gamma irradiation. Cempo Ireng is one type of rice in Indonesia with plant properties such as tall plant, long harvesting periods, and susceptibility to brown planthopper attacks. The research aims to obtain information and select M3 mutant strains with properties of shorter plants, early harvesting, and brown planthopper resistance. The study was conducted from February-July 2022 in Klaten Regency, Central Java, Indonesia. The research material consisted of 3 mutant strains of black rice M3(GH8'), M3(GH51'), and M3(GH52'), with the control variety of Cempo Ireng. The experimental design used was a Randomized Complete Block Design without replication. Data were analyzed descriptively and used t-test, at a significance level of 5%. The results showed that M3 mutant black rice had better growth and yield components than the control plant. Brown planthopper attacks of M3 mutant strains decreased to 24% compared to the control.

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Mutagenic Effects of Gamma-rays and EMS on M1 Population of Rice Bean (Vigna umbellata Thunb, Ohwi & Ohashi)

Cited by 3 publications

References 4 publications

Assessment of mutagenic sensitivity in blackgram variety CO 6

Assessment of mutagenic sensitivity in blackgram variety CO 6

M₂ plants derived from different tillers of a chemically mutagenized rice M₁ plant carry independent sets of mutations

Selection of M3 mutant strains from 200 gy gamma irradiation on Cempo Ireng

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Mutagenic Effects of Gamma-rays and EMS on M1 Population of Rice Bean (Vigna umbellata Thunb, Ohwi & Ohashi)

Cited by 3 publications

References 4 publications

Assessment of mutagenic sensitivity in blackgram variety CO 6

Assessment of mutagenic sensitivity in blackgram variety CO 6

M2 plants derived from different tillers of a chemically mutagenized rice M1 plant carry independent sets of mutations

Selection of M3 mutant strains from 200 gy gamma irradiation on Cempo Ireng

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M₂ plants derived from different tillers of a chemically mutagenized rice M₁ plant carry independent sets of mutations