Mutagenic evaluation and spectroscopic characterization of flavonoidal fraction of Apium leptophyllum (Pers.) fruit

Sahoo, Himanshu Bhusan; Patro, Saroj Kumar; Sagar, Rakesh; Santani, D. D.

doi:10.4103/2231-0738.153798

Cited by 6 publications

(6 citation statements)

References 2 publications

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“…It was conducted as per the procedure of Schmid. [10][11][12] Needle was inserted into the upper end of femur and 0.5ml of fetal bovine serum was injected into the lower epiphyseal end. Collected bone marrow samples were centrifuged at 1000 rpm for 8 min.…”

Section: In Vivo Micronucleus Testmentioning

confidence: 99%

Genotoxic evaluation of ceftriaxone by in vivo micronucleus test in albino mice

Dayana

Manasa

2018

Int J Basic Clin Pharmacol

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Background: Genotoxicity screening of drugs is essential. It is mandatory for new drugs. However, screening of drugs already in use is also necessary. Several cephalosporins are reported to induce chromosomal aberrations in previous studies. But there is paucity of data regarding the genotoxic potential of ceftriaxone. Hence the present study was undertaken to evaluate the genotoxic potential of ceftriaxone, a third generation cephalosporin, by micronucleus assay in albino mice.Methods: In vivo micronucleus test was performed with mice bone marrow after intraperitoneal injection of ceftriaxone at 100mg/kg BW and 200mg/kg BW at 24 hr and 48 hr harvest time. Mice bone marrow was harvested, and slides were prepared. The percentage of micronucleated polychromatic erythrocytes (% MnPCE) and the ratio of polychromatic erythrocytes to normochromatic erythrocytes (PCE:NCE) were determined. The data from ceftriaxone treated groups was compared with control group and analyzed using ANOVA followed by Dunnett's test.Results: Ceftriaxone at the dose of 100mg/kg BW and 200mg/kg BW did not exhibit any significant increase in the percentage of micronucleated polychromatic erythrocytes. It also did not decrease the ratio of polychromatic erythrocytes to normochromatic erythrocytes significantly.Conclusions: The present study demonstrates that ceftriaxone is not genotoxic in in vivo micronucleus study in albino mice at a dose of 100mg/kg BW and 200mg/kg BW.

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Section: In Vivo Micronucleus Testmentioning

confidence: 99%

Genotoxic evaluation of ceftriaxone by in vivo micronucleus test in albino mice

Dayana

Manasa

2018

Int J Basic Clin Pharmacol

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“…In India, it is cultivated in Andhra Pradesh, Gujarat, Madhya Pradesh, Jammu-Kashmir and Karnataka [7] . Traditionally, the fruit is widely used as an antinephritic, antirheumatic, carminative and is also beneficial for prevention of tumour, anorexia, vomiting, colic pain and mitch [8] . The volatile oil from the leaves possesses antimicrobial activity and radical scavenging activity [9] .…”

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confidence: 99%

“…Earlier scientific investigation showed that the fruits of this plant possesses antioxidant, chemopreventive and antimutagenic activity in mice [10] , [11] . Phytochemically, it contains volatile oils, coumarin derivatives, terpene hydrocarbons, phenolics and alkaloids, and is a rich source of flavonoids [8] , [10] . This plant is used in the treatment of diarrhoea and stomachaches due to its strong antifungal, antibacterial and anti-inflammatory property [12] , [13] .…”

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Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in rats

et al. 2016

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BackgroundApium leptophyllum (Pers.) is an annual herb with traditional appreciation for various pharmacological properties; however, the scientific information on this herb is insufficient. The aim of the present investigation was undertaken to evaluate flavonoidal fraction of A. leptophyllum fruit (FFALF) against diarrhoea on albino rats.MethodsThe antidiarrhoeal study was conducted by castor oil induce diarrhoea, prostaglandin E2 (PGE2) induced enteropooling and intestinal transit by charcoal meal test. The rats were divided into five groups (six/group). Group I served as control and received orally 2% acacia suspension; Group II served as standard and received orally loperamide (3 mg/kg) or atropine sulphate (5 mg/kg); Group III, IV and V served as test groups and received the FFALF at doses of 5, 10 and 20 mg/kg orally, respectively.ResultsIn castor oil-induced diarrhoeal model, the FFALF significantly (p < 0.001) reduced the frequency of diarrhoea, defecation and weight of faeces as well as increased the sodium–potassium ATPase (Na+K+ATPase) activity and decreased nitric oxide (NO) content in the small intestine. In prostaglandin induced enteropooling model, it significantly (p < 0.01) and dose dependently slowed the intestinal fluid accumulation by decreasing the masses and volumes of intestinal fluid where as in charcoal meal test, it decreased charcoal meal transit in gastrointestinal tract as compared with control.ConclusionsThe study reveals that the FFALF possess anti-diarrhoeal properties mediated through inhibition of hyper secretion and gastrointestinal motility which support the traditional use of the plant.

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“…[ 5 ] Traditionally, the fruit was widely used as an antinephritic, antirheumatic, carminative and was beneficial for prevention of tumor, anorexia, vomiting, colic pain, and mitch. [ 6 ] The volatile oil of the leaves possesses antimicrobial and radical scavenging activity. [ 7 ] Earlier scientific investigation showed that the fruits of this plant possess antioxidant, chemopreventive, and antimutagenic activity in mice.…”

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confidence: 99%

“…[ 8 9 ] Phytochemically, it contains volatile oils, coumarins, terpene hydrocarbons, phenolics, alkaloids, and is a rich source of flavonoids. [ 6 8 ] Again, the fruit was useful in bronchitis, cough, and asthma by various traditional practitioners of India. In addition, the fruits possess thermogenic and antispasmodic in asthmatic patients.…”

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Pharmacological evaluation of Apium leptophyllum (Pers.) on bronchial asthma

et al. 2016

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Objective:The present study was to investigate the antiasthmatic potential from the flavonoid fraction of Apium leptophyllum fruit (FFALF) to validate its traditional claim.Materials and Methods:The antiasthmatic activity of FFALF was evaluated by histamine or acetylcholine-induced bronchospasm model in guinea pigs, compound 48/80 induced mast cell degranulation in albino rats and histamine-induced tracheal contraction in guinea pig. The preconvulsion dyspnea time at 0th and 7th day at the dose of 100 and 200 mg/kg in guinea pig's bronchospasm model, the percentage of granulated and degranulated mast cell at the dose of 500, 750, and 1000 μg/ml in rats and tracheal contraction at the dose of 500, 750, and 1000 μg/ml in guinea pig were measured and compared with respective control groups.Results:The treatments of FFALF were significantly (P < 0.001) decreased the histamine/acetylcholine-induced bronchospasm, mast cell degranulation, and histamine-induced tracheal contraction as compared to inducer group. In addition, FFALF showed dose-dependent antiasthmatic activity in all the animals.Conclusion:Hence, this study suggested that the FFALF showed antiasthmatic activity probably by membrane stabilizing property as well as suppressing antibody production and inhibiting of antigen induced by histamine and acetylcholine.

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Mutagenic evaluation and spectroscopic characterization of flavonoidal fraction of Apium leptophyllum (Pers.) fruit

Cited by 6 publications

References 2 publications

Genotoxic evaluation of ceftriaxone by in vivo micronucleus test in albino mice

Genotoxic evaluation of ceftriaxone by in vivo micronucleus test in albino mice

Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in rats

Pharmacological evaluation of Apium leptophyllum (Pers.) on bronchial asthma

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