Mutagenic potential of water from Pelotas Creek in Rio Grande do Sul, Brazil

Abstract

ABSTRACT. Water resource degradation is one of mankind's greatest worries, as it causes direct and indirect damage to the associated biota. We initiated a water monitoring study in Pelotas Creek in 2003 in order to assess the mutagenic effect of the creek's waters. Allium cepa cells exposed to water samples and a chronically exposed macrophyte were analyzed, through evaluation of the mitotic index, mitotic anomalies, interphase anomalies, and total anomalies. Five points were chosen along the lower course of Pelotas Creek, from which water samples and floating pennywort (Hydrocotyle ranunculoides, Apiaceae) were collected in 2006 and 2007. The enteric bacterium Escherichia coli was found at all sampling points; in the physical-chemical analysis, a few variables exceeded permitted limits, pH (from 6 to 9), chloride (250 mg/L), hardness (from 10 to 200 mg CaCO 3 /L), and conductivity (100 µΩ/cm). There was an increased number of cytogenetic anomalies in exposed A. cepa cells and in the pennywort in 2006 relative to 2007, which may be explained by the increased rainfall, which was three times greater in 2007 at some stations than in 2006.

Ecotoxicological assessment of irrigation water for vegetables in a watershed region of Greater São Paulo

“…Besides, low values of dissolved oxygen (DO), high conductivity values and color, (Table 1) allow us to infer the existence of a high load of organic pollution likely originated from domestic sewage in these waters also confirmed by analysis of thermotolerant coliforms (Table 2).Similar results to these were also found by Santos et al, (2009) and Bianchi et al (2011).…”

“…In recent years, various studies testing the system Allium cepa has been conducted in order to evaluate the effects of industrial and domestic effluents into surface waters (Santos et al, 2009 ;Barbosa et al, 2010 ;Bianchi et al, 2011;Nunes et al, 2011;Düsman et al, 2013). Overall, the highest genotoxic activity values for superficial water samples were found in points 2 and 3, suggesting that these points had been compromised to a greater extent due to the presence of genotoxic compounds, a result that was also found in sediment samples.…”

Multi‐institutional experience of genetic diagnosis in Ecuador: National registry of chromosome alterations and polymorphisms

“…Our results are consistent with previous studies in México, Argentina, Chile and Colombia that reported 45,X monosomy as the most common karyotype associated with TS: 30%, 35%, 60%, and 73%, respectively (Cortés‐Gutiérrez, Dávila‐Rodríguez, Vargas‐Villarreal, & Cerda‐Flores, ; Del Rey et al, ; Román et al, ; Saldarriaga, Valencia, Fandiño‐Losada, Andrés Ruiz, & Isaza, ). In contrast, in a Brazilian register somatic mosaicism (53.6%) was more common than the classic karyotype (28.6%) (Duarte et al, ). TS diagnosis is usually postponed until adolescence when pubertal delay, infertility and amenorrhea are present.…”

“…DS and TS often evidenced great karyotype variability (Duarte et al, ; Gimeno‐Martos et al, ), which emphasized the importance of differential cytogenetic diagnosis confirmation. The rest of structural autosome rearrangements were similarly scarce and varied (Duarte et al, ; Gimeno‐Martos et al, ; Gómez‐Puente et al, ).…”

Genotoxicidade e Citotoxicidade da água do Rio Passaúna: Bioensaio com Allium Cepa e relação com níveis de cafeína

“…Uma das primeiras análises utilizando Allium cepa foi realizada por Levan (1938), quando os efeitos da colchicina foram investigados. Desde então, o teste vem sendo utilizado em avaliações ambientais, por exemplo, de amostras de águas de lagos (GRISOLIA et al, 2005;BARBOSA, 2008) e de rios (CASTRO E SOUSA et al, 2017;DÜSMAN et al, 2014;SILVA & NASCIMENTO, 2013;CIRCUNVIS et al, 2012;FERREIRA et al, 2012;BIANCHI et al, 2011;ME-NEGUETTI et al, 2011;MENDES et al, 2011;OLIVEI-RA et al, 2011;MANZANO, 2010;SANTOS et al, 2009;PERON et al, 2009;MASCHIO et al, 2009;LEME & MARIN-MORALES, 2008;CARITÁ & MARIN-MORALES, 2008;EGITO et al, 2007;AMARAL et al, 2007;MATSU-MOTO & MARIN-MORALES, 2005).…”