Mutagenicity and chemopreventive activities of Astronium species assessed by Ames test

Resende, Flávia Aparecida; Campos, Denise; Silva, Viviane Cândida da; Grandis, Rone Aparecido De; Souza, Leonardo Perez de; Leonardo, Carlos Sérgio; Rocha, Cláudia Quintino da; Santos, Lourdes Campaner dos; Vilegas, Wagner; Varanda, Eliana Aparecida

doi:10.1016/j.yrtph.2015.05.014

Cited by 15 publications

(13 citation statements)

References 36 publications

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“…The chemical composition analysis of M. urundeuva leaf dry decoction revealed the presence of 24 ubiquitous secondary metabolites, mainly simple phenolic acids and their esters, flavonoids, flavonoid glycosides, and gallotannins (Aquino et al ). Similar results with the leaf ethanol and hydroalcoholic extracts of M. urundeuva were reported by Silva et al () and Resende et al (), respectively. Usually, the presence of gallic acid derivatives, gallotannins, and polar flavonoids in the studied decoction should be an important information regarding the safety and possible biological activities related to the presence of these ubiquitous compounds.…”

Section: Discussionsupporting

confidence: 91%

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Genotoxic Assessment of the Dry Decoction of Myracrodruon urundeuva Allemão (Anacardiaceae) Leaves in Somatic Cells of Drosophila melanogaster by the Comet and SMART Assays

Amorim

Santana

Silva

et al. 2019

Environ and Mol Mutagen

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Medicinal plants are worldwide used as an efficient treatment of many diseases. Myracrodruon urundeuva Allemão (Anacardiaceae) is widely used Brazilian folk medicine to treat inflammations and infections of the female genital tract, conditions of the stomach and throat, and to heal wounds on the skin and mucous membranes. Several pharmacological properties of extracts and compounds isolated from M. urundeuva are found in the literature, corroborating its uses as antiulcer and gastroprotective, anti‐inflammatory and analgesic, as well as antimicrobial. Despite these many uses in traditional herbal medicine, there are few reports of its toxic‐genetic effect. This work aimed to investigate the genotoxic and mutagenic potential in vivo of the dry decoction of M. urundeuva leaves on somatic cells of Drosophila melanogaster, through the Comet assay and somatic mutation and recombination test (SMART). Six concentrations (0.5, 1.0, 2.0, 4.0, 8.0, and 16.0 mg/mL) were studied after feeding individuals for 24 hr in culture medium hydrated with extracts of M. urundeuva. In the Comet assay, all concentrations showed a genotoxic effect significantly higher than the negative control group, treated with distilled water. The two highest concentrations were also superior to the positive control group, treated with cyclophosphamide (1 mg/mL). In the SMART, there was a mutagenic effect at all concentrations tested, with a clear dose‐dependent relationship. Both recombination and mutation account for these mutagenic effects. The set of results indicate that the dry decoction of M. urundeuva leaves is genotoxic and mutagenic for D. melanogaster under the experimental conditions of this study. Environ. Mol. Mutagen. 61:329–337, 2020. © 2019 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 91%

“…The mutagenicity and antimutagenicity of hydroalcoholic extracts from M. urundeuva leaves and stem bark were assessed by the Ames test. None of the extracts any presented mutagenic effect and both exhibited a protective effect against different mutagens, either in the presence, or absence, of metabolic activation (Resende et al ).…”

Section: Introductionmentioning

confidence: 99%

Genotoxic Assessment of the Dry Decoction of Myracrodruon urundeuva Allemão (Anacardiaceae) Leaves in Somatic Cells of Drosophila melanogaster by the Comet and SMART Assays

Amorim

Santana

Silva

et al. 2019

Environ and Mol Mutagen

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“…No antimutagenic effect was recorded when inhibition was lower than 25%, a moderate effect for an inhibition value between 25% and 40%, and strong antimutagenicity for values greater than 40% [29].…”

Section: Plos Onementioning

confidence: 90%

“…Where T is the mean number of revertant colonies in the plate containing both mutagen and test compounds, M is the mean number of revertant colonies in the plate containing only the mutagen [29].…”

Section: Salmonella Mutagenicity Assay (Ames Test)mentioning

confidence: 99%

“…Results of antimutagenic activity with and without metabolic activation are presented respectively in Tables 1 and 2, as the mean of revertants/plates ± SD of three independent experiments (each one based on three replicates). Inhibition rate percentage was calculated [29]. Basically, significant moderate and strong antimutagenic effects exerted by F.angustifolia Vahl.…”

Section: Antimutagenicitymentioning

confidence: 99%

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Antimutagenic, antigenotoxic and antiproliferative activities of Fraxinus angustifolia Vahl. leaves and stem bark extracts and their phytochemical composition

et al. 2020

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In recent years, chronic degenerative diseases such as certain types of cancers, are becoming an evident issue. DNA damage has been for long recognized as a causal factor for cancer development because mutations or chromosomal aberrations affect oncogenes and tumor suppressor genes leading cells to malignant transformation and to the subsequent cancerous growth. Medicinal plants are often used for the prevention or treatment of various diseases with great scientific interest. Among the medicinal plants distributed in the Mediterranean region, Fraxinus angustifolia Vahl. has been used in traditional medicine for its remarkable curative properties. However, in spite of this popularity, little works have been performed on the activity so that further studies should be performed to investigate in depth the antimutagenic, antigenotoxic and antiproliferative activities of the plant. Thus, the present study was aimed to the evaluation of the potential antimutagenic, antigenotoxic and antiproliferative properties of leaves and stem bark extracts of this well-known tree. Antimutagenic activity was evaluated by Salmonella mutagenicity assay in Salmonella typhimurium TA98 and TA100 strains. The antigenotoxic potential was assessed by umu test in the strain of S. typhimurium TA1535/pSK1002. Antiproliferative activity was studied on human hepatoblastoma (HepG-2) and on breast adenocarcinoma (MCF-7) cell lines by MTT assay. Furthermore, the antiproliferative activity observed on cancer cells was compared with that on the human normal-like fibroblasts (TelCOFS02MA) and the selectivity index was calculated to understand if extracts were able to exert selective toxicity towards cancer cells. Moreover, phenolic compounds are plant substances with a large spectrum of biochemical activities with antioxidant, antimutagenic and anticarcinogenic effects. Based on the strong evidence of biological activities of phenolic compounds, the study was focused on the determination of total phenolics and flavonoids contents, and the phytochemical composition of the extracts assessed by LC/MS. The ethanol extracts of both leaves and stem barks showed

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Bark of Astronium lecointei Ducke trees from the Amazon: chemical and structural characterization

et al. 2021

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Mutagenicity and chemopreventive activities of Astronium species assessed by Ames test

Cited by 15 publications

References 36 publications

Genotoxic Assessment of the Dry Decoction of Myracrodruon urundeuva Allemão (Anacardiaceae) Leaves in Somatic Cells of Drosophila melanogaster by the Comet and SMART Assays

Genotoxic Assessment of the Dry Decoction of Myracrodruon urundeuva Allemão (Anacardiaceae) Leaves in Somatic Cells of Drosophila melanogaster by the Comet and SMART Assays

Antimutagenic, antigenotoxic and antiproliferative activities of Fraxinus angustifolia Vahl. leaves and stem bark extracts and their phytochemical composition

Bark of Astronium lecointei Ducke trees from the Amazon: chemical and structural characterization

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