Mutagenicity, antioxidant activity and nutritional content of long-life tomato

Silva, Regildo Márcio Gonçalves; Figueiredo, Célia Cristina Malaguti; Gomes, A.H.A.; Peixoto, Edison Carvalho Sandoval; Ferreira, Paulo Michel Pinheiro; Silva, Luciana Pereira

doi:10.14393/bj-v32n4a2016-32806

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…These observations are in agreements with previous studies indicated that free radicals arising during radiation-exposure can lead to DNA damage. If overproduction of theses radicals occurs, oxidative damage could lead to radiation-induced cytotoxicity; and MNi expression (HALLIWELL, 2002).So, determination of micronucleated cells and chromosome aberrations frequencies has a valuable role in monitoring populations exposed to natural or synthetic mutagenic agents (SILVA et al, 2016). Khabour et al (2013) suggested that various vitamin E isoforms, including gamma tocotrienol, protect against cytogenetic damage in humans.…”

Section: Discussionmentioning

confidence: 99%

Radioprotective role of α-tocotrienol against oxidative damage in γ-irradiated human blood cultures

Ahmed¹,

Montaser²,

Mohamed³

et al. 2018

Biosci. J.

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Tocotrienols are members of the natural vitamin E family which is considered one of important fat soluble vitamins. The tocotrienols react with free radicals, which are the main cause of oxidation damage to cell membranes, without formation of other free radicals in the process. All natural forms of tocotrienols have the ability to regulate peroxidation reactions and to control free radicals production within the body. This study aimed to assess the antimutagenic and antioxidant ability of α-tocotrienol at a working dose (0.04 mg/ ml) through cytogenetic (Micronucleus test) study and biochemical analysis including Caspase-3, Superoxide dismutase (SOD), Catalase (CAT) activities and Nitric Oxide (NO) concentration in γ-irradiated human blood cultures. The treatment time was 72 hrs post-irradiation with gamma rays at dose of 3 Gy. Triple blood cultures for each blood sample were set up. Ionizing irradiation induces a significant increase in micronuclei (MNi) frequencies, and nucleuplasmic bridge (N bridge) accompanied by a significant rise in Caspase-3 activity and NO concentration. Furthermore, SOD and CAT activities showed significant decrease. αtocotrienol treatment results into a decrease of MNi and N bridges numbers, enhancement of SOD and CAT activities and improvement of both NO and Caspase-3 levels, compared to irradiated cells which not treated with α-tocotrienol. The present results reveal the antimutagenic and the anti-oxidant effects of α-tocotrienol against γ-irradiation.

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Section: Discussionmentioning

confidence: 99%

Radioprotective role of α-tocotrienol against oxidative damage in γ-irradiated human blood cultures

Ahmed¹,

Montaser²,

Mohamed³

et al. 2018

Biosci. J.

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“…High-performance liquid chromatography (HPLC) is the most widely used technique in food analysis for measuring vitamins, carbohydrates, additives (antioxidants, colorants, artificial sweeteners, etc. ), proteins, amino acids, mycotoxins, triglycerides (TGs) in fats and oils, lipids, chiral compounds, and pigments (carotenoids, anthocyanines) (Chang et al, 1995;Tarrago-Trani et al, 2012;Silva et al, 2016;Colak et al, 2022).…”

mentioning

confidence: 99%

Gamma rays induced enhancement in the phytonutrient capacities of tomato (Solanum Lycopersicum L.)

et al. 2023

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One of the most important problems for the field-grown Ayaş tomato (Solanum lycopersicum Ayas population), which is preferred for consumption in Central Anatolia because of its aromatic taste, is that the shelf life is short because of insufficient fruit firmness. Therefore, a study was initiated to develop high-quality lines and variety candidates through mutation breeding in the current Ayaş population. In this study, the effective mutation dose (EMD50) was found to be 150 Gy for seeds using a Cobalt-60 gamma ray source. The main mutant population was generated by applying EMD50. During the study, mutant lines selected by following the classical mutation breeding stages were evaluated in terms of yield and quality traits (antioxidant content, total soluble solid amount, fruit firmness, Brix, etc.) starting from the M4 stage. One of our aims was to determine and improve the phytonutrient content of field-grown tomatoes. The fruits of selected lines at the M4 stage were extracted by liquid-liquid partition and accelerated solvent extraction techniques and analyzed by High Performance Liquid Chromatography (HPLC) in this study. It was found that 28 of the 29 mutant lines were significantly different from the control and mutant lines. Mutant lines 9-22, 8-90, 8-135, and 8-127 were determined to be the most promising for commercialization.

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Mutagenicity, antioxidant activity and nutritional content of long-life tomato

Cited by 2 publications

References 33 publications

Radioprotective role of α-tocotrienol against oxidative damage in γ-irradiated human blood cultures

Radioprotective role of α-tocotrienol against oxidative damage in γ-irradiated human blood cultures

Gamma rays induced enhancement in the phytonutrient capacities of tomato (Solanum Lycopersicum L.)

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