Mutagenicity assessment of Salacia chinensis by bacterial reverse mutation assay using histidine dependent Salmonella typhimurium tester strains

Nagesh, Madhuranthakam Reddi; Gatasheh, Mansour K.; Hoda, Nasrul; Vijayakumar, Natesan

doi:10.1016/j.sjbs.2022.103370

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…Previous studies conducted on the root extract of Salacia chinensis using Ame's test, in the presence of the s9 mix, the same method as used in our study and absence of the S9 Mix (rat liver extract after treatment with metabolic enzyme-inducing drugs) not used in our study, indicated that the root extract was not mutagenic [9]. Experimental results conducted in the presence of the S9 mix when positive is an indication that the product under test requires metabolic activation to cause mutation and when testing complex mixtures, the Ames miniaturized, microplate fluctuation format test (MPF) test is advantageous [20,21].…”

Section: Discussionsupporting

confidence: 53%

“…Any chemical substance (compounds) that is capable of causing the organism to mutate is considered mutagenic and possibly carcinogenic [2,3]. Evaluation of herbal medicine products which contains polychemical substances (compounds) for their mutagenic potential has not been a common practice Worldwide but of late, several products have been evaluated for their mutagenic potentials [4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Mutagenic Potential of Artavol® Using the Ame’s Test

Oloro,

Kasazza,

P’okello

et al. 2024

EJMP

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Background: The worldwide increase in the use of herbal medicinal - products calls for their safety testing to protect the public from unintended hazardous effects. However, most are not tested, putting the public at risk. The modified Ames ISO test is a useful resource for determining the mutagenic potential of medicinal products and was used in this study to determine the safety of an herbal tea like Artavol®. Methods: This study used Ame's Modified ISO test to analyze aqueous extract of Artavol®, in a Level II biosafety cabinet. Artavol® was extracted using the infusion method 1,2, freeze dried, diluted to concentrations of 125µg, 250µg, and 500µg and used in the study. Mutagenicity was tested by culturing Salmonella typhimurium TA98 and TA100. Results were considered by observing colour change in the wells of the microtitre plates from purple to yellow indicating mutation. Valid results were determined by comparing negative and positive control plates after 3 days of incubation, with positive results showing a color change from purple to yellow. Results: Colour changes were not observed in all wells containing 125µg, 250µg, and 500µg of Artavol® up to day 3, while negative control showed color changes equivalent to 80% for TA98 and 70% for TA100. Conclusion: Aqueous extract of Artavol® is not mutagenic. Further safety test such as sub-chronic toxicity study ad teratogenicity studies are recommended to provide more safety data on the product.

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Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Evaluation of the Mutagenic Potential of Artavol® Using the Ame’s Test

Oloro,

Kasazza,

P’okello

et al. 2024

EJMP

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Phytochemical and pharmacological insights into Salacia chinensis L. (Saptarangi): an underexplored important medicinal plant

Nikule,

Nikam,

Borde

et al. 2024

Discov. Plants

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Salacia reticulata: An Integrative Review of its Antioxidant, Lipid-Lowering, and Glucose-Regulating Properties in Diabetes and Obesity

Muskan,

Panwar,

Chopra

et al. 2025

NPJ

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Abstract:: Across the globe, approximately half of the population diagnosed with diabetes use complementary medicines for the treatment of diabetes. Salacia reticulata (family Hypocrataceae), is an indigenous woody climber flowering plant commonly employed within the Ayurvedic healthcare framework for addressing diabetes and obesity. It is also known as KothalaHimbutu and grows in the dry zone forests of India and Sri Lanka. It is documented to exhibit antioxidant, lipid-lowering, hypertrophy- reducing, and fibrosis-inhibiting properties and hepatoprotective activity. We critically analyze the available in-vitro, animal, and clinical research supporting the utilization of Salacia reticulata in managing type 2 diabetes and obesity. Compounds that have been recognized for their ability to counteract diabetes include salacinol, kotalanol, ponkoranol, and salaprinol. Various invitro research depicted salacia's capacity to impede intestinal alpha-glucosidase function. Furthermore, it enhances the breakdown of stored fat (lipolysis) and reduces insulin resistance by increasing the production of messenger RNA for hormone-sensitive lipase (HSL) as well as adiponectin, respectively, in the mouse mesenteric fat. Salacia reticulata treatment up-regulates the lipolysis factors while downregulating the 3T3-L1 adipocytes lipogenesis factors. Both animal studies and clinical research consistently showed significant improvement in levels of glucose when fasting compared to being exposed to sucrose and maltose. Furthermore, 6 weeks to 3 months of treatment showed a substantial reduction in the HbA1C and plasma Insulin. Salacia reticulata efficiently decreases obesity and insulin resistance while enhancing glucose metabolism therefore, more substantial evidence derived from meticulously designed research is necessary to confirm its effectiveness and safety. Moreover, the research aimed at improving the growth of callus, increasing polyphenolic content, promoting mangiferin synthesis, and assessing the biological properties of the salaciagenus suggests its potential as a valuable source for the industrial production of important industrial secondary compounds. At the same time, data indicate cumulative knowledge, highlighting its strong antioxidant effect and unveiling its capabilities without impacting natural reserves.

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Mutagenicity assessment of Salacia chinensis by bacterial reverse mutation assay using histidine dependent Salmonella typhimurium tester strains

Cited by 3 publications

References 14 publications

Evaluation of the Mutagenic Potential of Artavol® Using the Ame’s Test

Evaluation of the Mutagenic Potential of Artavol® Using the Ame’s Test

Phytochemical and pharmacological insights into Salacia chinensis L. (Saptarangi): an underexplored important medicinal plant

Salacia reticulata: An Integrative Review of its Antioxidant, Lipid-Lowering, and Glucose-Regulating Properties in Diabetes and Obesity

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