Mutagenicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin and perfluoro-n-decanoic acid in L5178Y mouse-lymphoma cells

Rogers, Alix; Andersen, Melvin E.; Back, Kenneth C.

doi:10.1016/0165-7992(82)90192-0

Cited by 30 publications

(4 citation statements)

References 10 publications

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“…PFDA was not mutagenic in the Chinese hamster ovary (CHO)/HGPRT forward mutation assay when cells were exposed to concentrations ranging from 0.01 to 0.16 mg/mL in the absence of S9 mix or from 0.005 to 0.1 mg/mL in the presence of S9 mix 89 . PFDA was also negative in the mouse lymphoma L5178Y/tk +/forward mutation assay after 24 hours of exposure at concentrations ranging from 0.01 to 100 µg/mL in the absence of S9 mix 98 . Sister chromatid exchanges were not induced in CHO-WBL cells exposed to PFDA for 2.5 hours at concentrations ranging from 1.67 to 50 µg/mL in the absence of S9 mix or when exposed to PFDA for 2 hours at concentrations ranging from 5 to 167 µg/mL with S9 mix 89 .…”

Section: Genetic Toxicitymentioning

confidence: 98%

NTP Technical Report on the Toxicity Studies of Perfluoroalkyl Carboxylates (Perfluorohexanoic Acid, Perfluorooctanoic Acid, Perfluorononanoic Acid, and Perfluorodecanoic Acid) Administered by Gavage to Sprague Dawley (Hsd:Sprague Dawley SD) Rats

2019

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Section: Genetic Toxicitymentioning

confidence: 98%

NTP Technical Report on the Toxicity Studies of Perfluoroalkyl Carboxylates (Perfluorohexanoic Acid, Perfluorooctanoic Acid, Perfluorononanoic Acid, and Perfluorodecanoic Acid) Administered by Gavage to Sprague Dawley (Hsd:Sprague Dawley SD) Rats

2019

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“…Thus, in the present study we constructed CA-containing dendrimers, which were shown to self-assemble into micelles at low micromolar concentrations 22 . Similarly, DA-conjugated 24 peptides have been reported to possess greater affinity for various cancer cells, which led us to trial this as an alternative dual targeting and self-assembling lipid, alongside CA. Using SPPS 6 , a panel of asymmetric peptide dendrimers, varying in generation and head group chemistry, in the presence or absence of lipid (CA and DA) conjugation were synthesized and characterized as illustrated in Fig.…”

Section: Introductionmentioning

confidence: 99%

Self-assembling asymmetric peptide-dendrimer micelles – a platform for effective and versatile in vitro nucleic acid delivery

Kokil

Veedu

et al. 2018

Sci Rep

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Despite advancements in the development of high generation cationic-dendrimer systems for delivery of nucleic acid-based therapeutics, commercially available chemical agents suffer from major drawbacks such as cytotoxicity while being laborious and costly to synthesize. To overcome the aforementioned limitations, low-generation cationic peptide asymmetric dendrimers with side arm lipid (cholic and decanoic acid) conjugation were designed, synthesized and systematically screened for their ability to self-assemble into micelles using dynamic light scattering. Cytotoxicity profiling revealed that our entire asymmetric peptide dendrimer library when trialled alone, or as asymmetric dendrimer micelle-nucleic acid complexes, were non-cytotoxic across a broad concentration range. Further, the delivery efficiency of asymmetric peptide dendrimers in H-4-II-E (rat hepatoma), H2K (mdx mouse myoblast), and DAOY (human medulloblastoma) cells demonstrated that cholic acid-conjugated asymmetric dendrimers possess far superior delivery efficiency when compared to the commercial standards, Lipofectamine 2000 or Lipofectin®.

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“…The aim of this study was to estimate the genetic risk of two environmental dioxin mixtures in comparison to 2,3,7,8-tetradichlorodibenzo-p-dioxin (TCDD). It is known that TCDD is carcinogenic (1-5), but it is nearly ineffective in genotoxicity tests (6)(7)(8)(9)(10)(11)(12). Therefore, it did not seem useful to perform another test for mutagenicity.…”

Section: Introductionmentioning

confidence: 99%

Genetic effects of dioxins in the spot test with mice.

Fahrig

1993

Environ Health Perspect

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More than any other environmental chemicals, dioxins have been in the limelight of public interest for about 10 years. In addition to carcinogenicity, genetic risk is a cause for concern. Mutagenicity tests performed so far do not give a clear picture. The mutagenic potential of dioxins has to be considered weak or absent. Therefore, it seemed profitable to investigate comutagenicity and co-recombinogenicity of dioxins more thoroughly. The only useful method for investigating comutagenicity and co-recombinogenicity of dioxins in vivo is the spot test with mice. In this test system, a number of cocarcinogens and tumor promoters have shown comutagenic or co-recombinogenic effects. In the present study, tetrachlorodibenzo-p-dioxin (TCDD) and two environmental dioxin mixtures [pentachlorodibenzodioxin (PCDD) 1 and 2] were tested for genetic activity. Given alone, no mutagenic or recombinogenic effects could be observed. In combination with the carcinogenic mutagen ethyl nitrosourea (ENU) at concentrations of 128 micrograms/kg for PCCD 2, 314 micrograms/kg for PCDD 1, and 3 micrograms/kg for TCDD, a doubling of the genetic effectiveness of ENU was observed. The genetic risk can roughly be considered as 1:0.02 for TCDD:PCDD 2 and 1:0.01 for TCDD:PCDD 1. While PCDD 1 and 2 seem to enhance the mutagenic as well as the recombinogenic potential of ENU, TCDD showed mainly co-recombinogenic and antimutagenic activity. This characteristic indicates that TCDD is mainly a tumor promoter.

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Mutagenicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin and perfluoro-n-decanoic acid in L5178Y mouse-lymphoma cells

Cited by 30 publications

References 10 publications

NTP Technical Report on the Toxicity Studies of Perfluoroalkyl Carboxylates (Perfluorohexanoic Acid, Perfluorooctanoic Acid, Perfluorononanoic Acid, and Perfluorodecanoic Acid) Administered by Gavage to Sprague Dawley (Hsd:Sprague Dawley SD) Rats

NTP Technical Report on the Toxicity Studies of Perfluoroalkyl Carboxylates (Perfluorohexanoic Acid, Perfluorooctanoic Acid, Perfluorononanoic Acid, and Perfluorodecanoic Acid) Administered by Gavage to Sprague Dawley (Hsd:Sprague Dawley SD) Rats

Self-assembling asymmetric peptide-dendrimer micelles – a platform for effective and versatile in vitro nucleic acid delivery

Genetic effects of dioxins in the spot test with mice.

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