Mutagenicity of airborne particulate organic material from urban and industrial areas of São Paulo, Brazil

Sato, Maria Inês Z.; Umbuzeiro, Gisela de Aragão; Coimbrão, Carlos Alberto; Coelho, Maria Cristina L.S.; Sanchez, Petra Sanchez; Alonso, C. D.; Martins, Maria Therezinha

doi:10.1016/0165-1161(95)00035-6

Cited by 47 publications

(23 citation statements)

References 36 publications

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“…Studies performed with TA98NR and TA98/1,8-DNP 6 in areas contaminated by vehicular emissions and industrial activity in Rio Grande do Sul State of Brazil showed similar results: a reduction of 10-95% for TA98NR and a reduction of 61-85% for TA98/1,8-DNP 6 (Vargas et al, 1998;Ducatti and Vargas, 2003). Similar results were also detected in the industrial city of Cubatão, São Paulo (Sato et al, 1995). Meanwhile, an absence of mutagenicity was observed at site 1, which is characteristic of residential areas with a low flow of vehicles.…”

Section: Salmonella/microsome Assaysupporting

confidence: 70%

“…Among these, the mono-and dinitro-PAHs associated with oxygenated PAH have been correlated with direct frameshift mutagenic activity (Sato et al, 1995;Vargas et al, 1998;Ducatti and Vargas, 2003). In the present study, the contribution of mono-and di-nitroarenes to direct mutagenic activity was investigated though the Salmonella/microsome assay with strains TA98NR and TA98/1,8-DNP 6 , respectively.…”

Section: Salmonella/microsome Assaymentioning

confidence: 99%

“…The contributions of nitroarenes to direct mutagenic activity are commonly investigated through the Salmonella/microsome assay with the following S. enterica serovar Typhimurium strains: TA98 (frameshift strain) and the derivative strains TA98/1.8-DNP 6 (O-acetyltransferase-deficient), TA98NR (nitroreductase-deficient) (Rosenkranz and Mermelstein, 1980), YG1021 (nitroreductase-overproducing), and YG1024 (O-acetyltransferase-overproducing) (Watanabe et al, 1989(Watanabe et al, , 1990. Genotoxic evaluation studies of particulate matter using these strains have been conducted previously (Sato et al, 1995;Vargas et al, 1998;Ducatti and Vargas, 2003;de Aragão Umbuzeiro et al, 2008;Coronas et al, 2009;Pereira et al, 2010;Rainho et al, 2012Rainho et al, , 2013a.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the sensitivity of strains of Salmonella enterica serovar Typhimurium in the detection of mutagenicity induced by nitroarenes

et al. 2014

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ABSTRACT. The use of strains of Salmonella enterica serovar Typhimurium with different metabolic capacities can indicate the class or classes of compounds present in an environmental sample and enable the diagnosis of the mutagenic activity of these pollutants adsorbed on particulate matter (PM) in the air. In the present study, the sensitivity of Salmonella strains TA98NR, TA98/1,8-DNP 6 , YG1021, and YG1024 to detect nitro compounds adsorbed on samples of PM 2.5 was compared from three sites in Rio de Janeiro city. Samples were collected using a high-volume sampler at three sites: one with light traffic and two with heavy traffic. The assays were performed in the presence of 10-50 μg/ plate organic extracts with and without exogenous metabolization. The YG1021 and YG1024 strains showed the highest rev/m 3 values, confirming their enhanced sensitivity. As YG1024 also demonstrated sensitivity to nitro and amino compounds, we suggest its use in research into environmental contamination.

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Section: Salmonella/microsome Assaysupporting

confidence: 70%

Section: Salmonella/microsome Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of the sensitivity of strains of Salmonella enterica serovar Typhimurium in the detection of mutagenicity induced by nitroarenes

et al. 2014

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“…Using S. typhimurium TA98, Zhao et al [2002] also found that S9 reduces the mutagenicity of dichloromethane-extracted TSP, whereas De Martinis et al [1999] reported that the effect of S9 is different for acetone-extracted PM (reduced mutagenicity) and for dichloromethane-extracted material (stimulated mutagenicity). Also, several other studies report an increased mutagenicity in the presence of S9 [Bronzetti et al, 1997;Nielsen et al, 1999;Cerna et al, 2000;Topinka et al, 2000], an effect that Binkova et al [2003] could detect only in PM sampled during winter [Buschini et al, 2001], or report neither a stimulating nor a reducing effect [Sato et al, 1995].…”

Section: Discussionmentioning

confidence: 97%

Genotoxicity and physicochemical characteristics of traffic‐related ambient particulate matter

Kok

Hogervorst

Briedé

et al. 2005

Environ and Mol Mutagen

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Exposure to ambient particulate matter (PM) has been linked to several adverse health effects. Since vehicular traffic is a PM source of growing importance, we sampled total suspended particulate (TSP), PM(10), and PM(2.5) at six urban locations with pronounced differences in traffic intensity. The mutagenicity, DNA-adduct formation, and induction of oxidative DNA damage by the samples were studied as genotoxicological parameters, in relation to polycyclic aromatic hydrocarbon (PAH) levels, elemental composition, and radical-generating capacity (RGC) as chemical characteristics. We found pronounced differences in the genotoxicity and chemical characteristics of PM from the various locations, although we could not establish a correlation between traffic intensity and any of these characteristics for any of the PM size fractions. Therefore, the differences between locations may be due to local sources of PM, other than traffic. The concentration of total (carcinogenic) PAHs correlated positively with RGC, direct and S9-mediated mutagenicity, as well as the induction of DNA adducts and oxidative DNA damage. The interaction between total PAHs and transition metals correlated positively with DNA-adduct formation, particularly from the PM(2.5) fraction. RGC was not associated with one specific PM size fraction, but mutagenicity and DNA reactivity after metabolic activation were relatively high in PM(10) and PM(2.5), when compared with TSP. We conclude that the toxicological characteristics of urban PM samples show pronounced differences, even when PM concentrations at the sample sites are comparable. This implies that emission reduction strategies that take chemical and toxicological characteristics of PM into account may be useful for reducing the health risks associated with PM exposure.

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“…During period II, the presence of nitroarenes was also detected at all the sites, but dinitroarenes were only detected at sites 2 and 3. Nitroarenes and dinitroarenes result from direct emissions of diesel combustion and can be produced by atmospheric reactions of PAH with gaseous copollutants found in photochemical smog [43,44]. Studies performed in the urban region of Porto Alegre detected a similar response during the hot season, when there is marked mutagenic activity due to the presence of mono and dinitroarene compounds in different sizes of atmospheric particles such as PTS, PM10 and PM2.5 [4,45].…”

Section: Discussionmentioning

confidence: 66%

Seasonal variations in the level of mutagenicity: an assessment of respirable particulate matter in Rio de Janeiro, Brazil

Rainho¹,

Corrêa²,

Mazzei³

et al. 2014

WIT Transactions on Ecology and the Environment

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Respirable particles (PM2.5) can become associated with organic matter containing several compounds such as polycyclic aromatic hydrocarbons (PAHs). Many PAHs have been identified as cancer-inducing chemicals. The mutagenicity of airborne particles is generally associated with PAHs, but recent reviews show that PAHs may not be the predominant mutagens in atmospheric pollution, and that nitroaromatic compounds, aromatic amines and aromatic ketones, often found in moderately polar or highly polar organic fractions, are potent mutagens. Nitro-polycyclicaromatics (nitro-PAHs) are persistent environmental mutagens and can be found in airborne suspended particles from direct sources such as diesel and gasoline exhausts, or may be products of atmospheric reactions in the presence of NO 2 and NO 3 radicals. In the present work we compared PAH levels and mutagenicity using gas chromatography spectrometry and the Salmonella/microsome assay on organic extracts of PM2.5 for Salmonella typhimurium strain TA98. The samples were collected in two periods: (I) July to October 2010 and (II) November 2010 to May 2011 at three sites in Rio de Janeiro -(1) low urban traffic at the University campus; (2) heavy . Mutagenic frameshift responses in the absence of metabolic activation were detected at all the sites in periods I and II. In the presence of metabolic activation this response was observed for all three sites in period I, but only for site 3 in period II. Nitroarenes and dinitroarenes were detected at all three sites in period I. In period II, the presence of nitroarenes was also detected at all sites, but dinitroarenes were only detected at sites 2 and 3. The information generated in this study shows that different levels of PAHs and nitroderivatives, influenced by seasonal variations in climatic conditions, probably contribute to the detected airborne mutagenicity.

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Mutagenicity of airborne particulate organic material from urban and industrial areas of São Paulo, Brazil

Cited by 47 publications

References 36 publications

Comparison of the sensitivity of strains of Salmonella enterica serovar Typhimurium in the detection of mutagenicity induced by nitroarenes

Comparison of the sensitivity of strains of Salmonella enterica serovar Typhimurium in the detection of mutagenicity induced by nitroarenes

Genotoxicity and physicochemical characteristics of traffic‐related ambient particulate matter

Seasonal variations in the level of mutagenicity: an assessment of respirable particulate matter in Rio de Janeiro, Brazil

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