Mutagenicity of particulate matter fractions in areas under the impact of urban and industrial activities

Lemos, Andréia Torres de; Coronas, Mariana Vieira; Rocha, Jocelita Aparecida Vaz; Vargas, Vera Maria Ferrão

doi:10.1016/j.chemosphere.2012.05.100

Cited by 32 publications

(14 citation statements)

References 33 publications

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“…The results for nitroarenes and aromatic amines were higher at Site NO. The maximum values for each strain were also higher than the maximum values observed in previous studies in the state for 4 areas (Triunfo, Porto Alegre, Rio Grande and Santo Antônio da Patrulha), (Coronas et al, 2016;Brito et al, 2013;Lemos et al, 2012;Silva et al, 2015).…”

Section: Discussioncontrasting

confidence: 62%

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Lemos

Flores

et al. 2016

Chemosphere

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Section: Discussioncontrasting

confidence: 62%

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Lemos

Flores

et al. 2016

Chemosphere

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“…Surface waters, such as in rivers and lakes, are often exposed to household, industrial and agricultural contaminants (Ohe et al, 2004;Pellacani et al, 2006;Žegura et al, 2009;Lemos et al, 2012), such as pesticides, heavy metals and household cleaning products that contribute to toxicity (Pereira et al, 2007;Vargas et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity assays as tools to assess water quality in the Sinos River basin

et al. 2015

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Cytotoxicity assays using cell cultures may be an alternative to assess biological toxicity of surface waters and may help to improve the control of water quality. This study compared two methods to prepare culture media for the exposure of Hep-2 cells to water samples collected from the Rolante River, an important affluent of the Sinos River. The toxicity was evaluated using the MTT and neutral red assays. Two methods were used to prepare culture media. In method 1, the sample was diluted at 1:1, 1:10, 1:100, 1:1000, 1:10.000 (v/v, sample/medium) in a standard culture medium; in method 2, water samples were used as the solvent for the culture medium, which was prepared at concentrations of 100, 80, 60, 40 and 20%. Semi-confluent cultures were then exposed to the media test for 24 hours, and cytotoxicity was determined immediately using the MTT and NR assays. Mitochondrial activity (MTT) was significantly lower at all concentrations in both methods, except at 1:1000 in method 1. However, the lysosome viability (NR) results revealed cytotoxicity only in the 1:1 sample of method 1. Both culture preparation methods were efficient and sensitive to the MTT assay, but method 2 seemed to be more adequate for the NR assay. The Rolante River has cytotoxic contaminants to Hep-2 cells, which may be one of the explanations for the poor water quality of the Sinos River basin.Keywords: cytotoxicity, Sinos River basin, samples preparation. Ensaios de citotoxicidade como ferramentas para avaliar a qualidade da água da bacia do Rio do Sinos ResumoOs ensaios de citotoxicidade utilizando culturas de células constituem uma alternativa para avaliar a toxicidade biológica de águas de superfície e podem auxiliar no controle da qualidade da água. Este estudo comparou dois métodos de preparação dos meios de cultura com amostras de água coletadas no rio Rolante, um importante afluente do Rio dos Sinos, para a exposição de células Hep-2. A toxicidade foi avaliada usando os ensaios do MTT e do vermelho neutro (VN). Dois métodos foram utilizados para preparar os meios de cultura. No método 1, a amostra foi diluída a 1:1, 1:10, 1:100, 1:1000 e 1:10.000 (v/v, amostra/ meio de cultivo) em um meio de cultura padrão; no método 2, as amostras de água foram utilizados como solventes para o meio de cultura, o qual foi preparado em concentração de 100% e nas diluições de 80, 60, 40 e 20%. Culturas semi-confluentes foram então expostas aos meios teste durante 24 horas, e a citotoxicidade foi determinada imediatamente usando os ensaios MTT e VN. A atividade mitocondrial (MTT) foi significativamente menor em todas as concentrações em ambos os métodos, exceto na diluição 1:1000 do método 1. No entanto, os resultados de viabilidade lisossomal (VN) revelaram citotoxicidade apenas no na diluição 1:1 do método 1. Ambos os métodos de preparação do meio cultura foram eficientes e sensíveis para o ensaio do MTT, mas o método 2 foi mais adequado para o ensaio do VN. O rio Rolante possui contaminantes citotóxicos para as células Hep-2, o que pode ser uma...

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“…The Kyoto sample presented slightly lower mutagenic potencies for all the three strains in relation to the data presented by . In general, the samples evaluated by and the samples from Limeira, Stockholm, and Kyoto presented higher mutagenic potencies (rev./μg of EOM and rev./m 3 ) than the TPS samples submitted to ultrasonication-based organic extraction with DCM and also were evaluated using the Salmonella/microsome assay in microsuspension with the same strains Alves, 2011;Lemos et al, 2011;.…”

Section: Resultsmentioning

confidence: 95%

Comparação do perfil de mutagenicidade e da composição química do material particulado atmosférico de Limeira, Estocolmo e Quioto

Maselli¹

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Atmospheric particulate matter (PM) is associated with various diseases and has recently been classified as carcinogenic to humans (Group 1) by the International Agency for Research on Cancer (IARC). Although the PM toxicity, including genotoxicity, is known to be related to particle size, the contribution of its chemical composition to the observed effects has not yet been fully elucidated. The size and composition of the particles are influenced by meteorological and climatic characteristics, especially the temperature and the period of solar radiation. The Salmonella/microsome assay is the most used for the evaluate of the mutagenicity of PM samples, however a small number of strains are used. The objective of this study was to investigate and compare the influence of the different atmospheric and climatic conditions of the cities of Limeira (Brazil), Stockholm (Sweden) and Kyoto (Japan) on mutagenicity profiles, using 11 strains with different selectivity, and chemical composition profiles of total particles suspended (TPS) composed samples collected during the winter of these cities. For the results to be directly compared, the same methodology was adopted, including the sampling procedure, the sample preparation protocol, the mutagenicity test protocol, and the chemical analysis techniques for the identification of polycyclic aromatic hydrocarbons (PAH) and their alkylated derivatives. Limeira presented the highest concentration of TPS (99.0 μg/m 3), followed by Kyoto (28.0 μg/m 3) and Stockholm (6.2 μg/m 3). Although the TPS concentration in Limeira was 16-fold higher than in Stockholm and 3.5-fold higher than in Kyoto, the percentage of extracted organic material (EOM) obtained was 9, 15 and 5%, respectively. The extracts from the TPS samples collected in the three cities presented mutagenic activity for all strains, both in the absence and presence of S9, except for TA102 that did not detect the mutagenic activity in any of the extracts, and for YG7108 in the presence of S9, only for the Limeira sample. Despite the differences in meteorological and climatic conditions of these cities, their mutagenicity profiles were similar. The lower mutagenic potency for YG7108 in the absence of S9 and the nonmutagenicity in the presence of S9 reveals the lower contribution of alkylating agents to the mutagenicity of the Limeira sample in relation to the other cities. Considering the selectivity of the strains used, we can also observe a greater contribution of compounds that cause DNA oxidative damage and of alkylating agents for the mutagenicity of the Kyoto sample. However, according to the responses of the strains that have increased activity of the nitroreductase (NR) and/or O-acetyltransferase (OAT) enzymes, YG1021, YG1024 and YG1041, show the highest contribution of nitroarenes and aromatic amines for the mutagenicity of the three cities, with emphasis on those mutagens that depend on OAT activation. The mutagenic potencies expressed as a function of EOM mass (rev./μg EOM) were similar for all samples. When exp...

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Mutagenicity of particulate matter fractions in areas under the impact of urban and industrial activities

Cited by 32 publications

References 33 publications

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Cytotoxicity assays as tools to assess water quality in the Sinos River basin

Comparação do perfil de mutagenicidade e da composição química do material particulado atmosférico de Limeira, Estocolmo e Quioto

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Mutagenicity of particulate matter fractions in areas under the impact of urban and industrial activities

Cited by 32 publications

References 33 publications

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Cytotoxicity assays as tools to assess water quality in the Sinos River basin

Comparação do perfil de mutagenicidade e da composição quí­mica do material particulado atmosférico de Limeira, Estocolmo e Quioto

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Comparação do perfil de mutagenicidade e da composição química do material particulado atmosférico de Limeira, Estocolmo e Quioto