Mutagenicity of sodium azide and its metabolite azidoalanine in Drosophila melanogaster

Sadiq, May F.; Owais, W.M.

doi:10.1016/s1383-5718(00)00079-6

Cited by 29 publications

(26 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NaN 3 is known as a powerful mutagen, which affects several organisms including bacteria, plants, and animals, and generally causes base-substitutions. Previous studies showed that the mutagenicity of NaN 3 is related to the production of L-azidoalanine (Sadiq & Owais, 2000). One reason for observing no antimutagenicity against NaN 3 may be that both the tested chalcones do not have sufficient capability to inhibit formation of L-azidoalanine. However, these compounds showed significant antimutagenic activity against 9-aminoacridine (9-AA)-induced mutagenesis in S. typhimurium TA1537.…”

Section: Genotoxic and Antigenotoxic Activity Evaluationmentioning

confidence: 96%

Two antigenotoxic chalcone glycosides fromMentha longifoliasubsp.longifolia

Güvenalp

Özbek

Karadayı

et al. 2014

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Mentha L. (Labiatae) species (mint) with their flavoring properties have been used in food industries for centuries. Besides they have a great importance in drug development and medicinal applications due to various bioactive compounds of several members of the genus. Objective: The aim of this study was to isolate bioactive compounds with antimutagenic potential by bio-guided fractionation and determine their structures by spectroscopic methods. Materials and methods: The structural elucidation of the isolated compounds was done based on spectroscopic methods, including MALDI-MS, UV, IR, and 2D NMR experiments, and the bioguided fractionation process was done by using the Ames/Salmonella test system. Henceforth, solely genotoxic and antigenotoxic potential of the new compounds were also confirmed up to 2 mM/plate by using the same test system. According to the antimutagenicity results, both new test compounds significantly inhibited the mutagenic activity of 9-aminoacridine in a dose-dependent manner at the tested concentrations from 0.8 to 2 mM/plate. (bR)-b,4,2 0 ,6 0 -Tetrahydroxy-4 0 -O-rutinosyldihydrochalcone showed the maximum inhibition rate as 75.94% at 2 mM/plate concentration. Conclusions: This is the first report that two new chalcone glycosides were isolated from Mentha longifolia subsp. longifolia and their antimutagenic potentials by using mutant bacterial tester strains. In conclusion, the two new chalcone glycosides showed a significant antigenotoxic effect on 9-aminoacridine-induced mutagenesis at tested concentrations.

show abstract

Section: Genotoxic and Antigenotoxic Activity Evaluationmentioning

confidence: 96%

Two antigenotoxic chalcone glycosides fromMentha longifoliasubsp.longifolia

Güvenalp

Özbek

Karadayı

et al. 2014

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…Escherichia coli , Saccharomyces cerevisiae , H. vulgare , O. sativa and several other plant species, but does not increase mutation frequencies in A. thaliana and is only marginally mutagenic in humans and animals (Sadiq and Owais 2000). In barley, sodium azide is mutagenic in acidic pH (pH = 3) and it primarily induces A/T to G/C transitions.…”

Section: Mutagensmentioning

confidence: 99%

TILLING - a shortcut in functional genomics

et al. 2011

View full text Add to dashboard Cite

Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN Genomes) was developed a decade ago as an alternative to insertional mutagenesis. It takes advantage of classical mutagenesis, sequence availability and high-throughput screening for nucleotide polymorphisms in a targeted sequence. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of its genome size and ploidy level. The TILLING protocol provides a high frequency of point mutations distributed randomly in the genome. The great mutagenic potential of chemical agents to generate a high rate of nucleotide substitutions has been proven by the high density of mutations reported for TILLING populations in various plant species. For most of them, the analysis of several genes revealed 1 mutation/200–500 kb screened and much higher densities were observed for polyploid species, such as wheat. High-throughput TILLING permits the rapid and low-cost discovery of new alleles that are induced in plants. Several research centres have established a TILLING public service for various plant species. The recent trends in TILLING procedures rely on the diversification of bioinformatic tools, new methods of mutation detection, including mismatch-specific and sensitive endonucleases, but also various alternatives for LI-COR screening and single nucleotide polymorphism (SNP) discovery using next-generation sequencing technologies. The TILLING strategy has found numerous applications in functional genomics. Additionally, wide applications of this throughput method in basic and applied research have already been implemented through modifications of the original TILLING strategy, such as Ecotilling or Deletion TILLING.

show abstract

“…NaN 3 causes point mutations in several organisms including bacteria, plants and animals [39][40][41]. A lot of studies showed that mutagenesis mechanism of NaN 3 is associated with its metabolite called L-azidoline [40][41][42].…”

mentioning

confidence: 99%