Mutagenicity studies with urine concentrates from coke plant workers.

Møller, Mona; Dybing, Erik

doi:10.5271/sjweh.2611

Cited by 36 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although Mielzy nska and Snit (1992) found an association with coal, other studies did not (Moller & Dybing, 1980;Recio et al, 1984). We have found high concentrations of the PAH metabolite 9-hydroxybenzo[a]pyrene, a known mutagen (Lubet et al, 1979), in urine samples collected from smoky coal-exposed subjects (Mumford et al, 1995); however, these samples were not assessed for mutagenicity.…”

Section: Protection Agencycontrasting

confidence: 56%

Elevated urinary mutagenicity among those exposed to bituminous coal combustion emissions or diesel engine exhaust

Wong

Vermeulen

Dai

et al. 2021

Environ and Mol Mutagen

View full text Add to dashboard Cite

Urinary mutagenicity reflects systemic exposure to complex mixtures of genotoxic/ carcinogenic agents and is linked to tumor development. Coal combustion emissions (CCE) and diesel engine exhaust (DEE) are associated with cancers of the lung and other sites, but their influence on urinary mutagenicity is unclear. We investigated associations between exposure to CCE or DEE and urinary mutagenicity. In two separate cross-sectional studies of nonsmokers, organic extracts of urine were evaluated for mutagenicity levels using strain YG1041 in the Salmonella (Ames) mutagenicity assay. First, we compared levels among 10 female bituminous (smoky) coal users from Laibin, Xuanwei, China, and 10 female anthracite (smokeless) coal users. We estimated exposure-response relationships using indoor air concentrations of two

show abstract

Section: Protection Agencycontrasting

confidence: 56%

Elevated urinary mutagenicity among those exposed to bituminous coal combustion emissions or diesel engine exhaust

Wong

Vermeulen

Dai

et al. 2021

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…Increased urinary mutagenicity has been reported in smokers and workers exposed to aromatic amines and benzidine in the rubber and dyes industries (19)(20)(21)). An elevated urinary mutagenicity was also observed among groups occupationally exposed to polycyclic aromatic hydrocarbons, including coke oven workers, chimney sweeps, and aluminum workers (22), although the negative findings on mutagenic activity in urine samples of workers exposed to polycyclic aromatic hydrocarbons have also been reported by several authors (23,24). Meanwhile, because many chemicals that are mutagenic (or carcinogenic) are also toxic, acute toxicity of urine has been used as a screening test in addition to mutagenicity test (18).…”

Section: Introductionmentioning

confidence: 83%

Repeated Measurements of Urinary Methylated/Oxidative DNA Lesions, Acute Toxicity, and Mutagenicity in Coke Oven Workers

Chao¹,

Wang

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

View full text Add to dashboard Cite

We conducted a repeated-measures cohort study of coke oven workers to evaluate the relationships between the traditional exposure biomarker, urinary 1-hydroxypyrene (1-OHP), and a series of biomarkers, including urinary 8-oxo-7,8-dihydro-2 ¶-deoxyguanosine (8-oxodG), N7-methylguanine (N7-MeG), acute toxicity, and mutagenicity. A total of eight spot urine samples were collected from each high-exposed (at topside oven area) and low-exposed workers (at side oven area) during the whole working cycle, which consisted of 6 consecutive days of working followed by 2 days off. Our results showed that the high-exposed workers had significantly higher urinary levels of 1-OHP, 8-oxodG, and N7-MeG compared with the low-exposed workers. Acute toxicity and mutagenicity of urine were also found to be markedly increased in the high-exposed workers, as determined by Microtox assay and Ames test, respectively. Multivariate regressions analysis revealed that the urinary 8-oxodG, N7-MeG, or acute toxicity was significantly correlated with 1-OHP concentrations. Overall, the present study showed that exposure to coke oven emissions increased oxidatively damaged DNA products and mutagenicity of urine, and for the very first time, such exposure was also found to increase DNA methylation and urinary acute toxicity. The potential source of methylating agents in coke oven emissions warrants further investigation. Additionally, with repeated measurements, the pattern of time course for urinary 1-OHP was found to be different from those of 8-oxodG and N7-MeG, as well as acute toxicity and mutagenicity. This finding implies that the single measurement that was often conducted in occupational healthy investigations should be used with certain precautions, because single measurement may fail to provide the proper information of interest. (Cancer Epidemiol Biomarkers Prev 2008;17(12):3381 -9)

show abstract

“…Other at-risk exposures for colorectal adenoma, such as dietary intake of protein-rich meals cooked at high temperature (i.e., thermic amines with pan-fried meats) show that oral intake may contribute to an increase in urinary mutagenicity, but this assertion needs further confirmation [Murray et al, 2001;Pavanello et al, 2002b;Peters et al, 2003]. Published negative results [Moller and Dyhing, 1980;Recio et al, 1984;Reuterwall et al, 1991] were presumably due to the low sensitivity of most urinary mutagenicity assays [Clonfero et al, 1995] employing Salmonella strains that are not specifically engineered for the detection of heterocyclic amine exposures. Moreover, increments in urinary mutagenicity found in certain occupational or environmental studies are of doubtful significance due to the lack of control for confounding factors such as tobacco smoke [Pasquini et al, 1982;DeMeo et al, 1995;Sardas et al, 1997;Sorsa and Anderson, 1996] and diet [DeMeo et al, 1987;Heussener et al, 1985;Kriebel et al, 1983].…”

Section: Introductionmentioning

confidence: 92%

Mutagenic activity of overnight urine from healthy non‐smoking subjects

Pavanello

Lupi

Pulliero

et al. 2007

Environ and Mol Mutagen

View full text Add to dashboard Cite

Urinary mutagenicity was evaluated in relation to environmental mutagen exposure (i.e., diet, indoor/outdoor activities, residential area etc.) on the day prior to sample collection, and also considering factors that contribute to the variability of Salmonella mutagenicity assay results. Overnight urine samples from 283 healthy non-smoking residents of northeast Italy (46% males, 20-62 years) were analyzed for mutagenicity on sensitive Salmonella typhimurium strain YG1024 with S9 mix employing the preincubation version of the plate incorporation assay (i.e., the Salmonella reverse mutation test). Urinary mutagenicity varied between 0.02 and 9.84 rev/ equiv. ml, and 7% of samples were positive (i.e., sample elicited a two-fold increase in revertants). There was an evident increase in mutagenicity in subjects with increased intake of mutagen-rich meals (n = 80) (P < 0.01 and positive urine 13% vs. 5%, P = 0.025). Indoor-exposed subjects (n = 65) also showed a higher percentage of positive urine (14% vs. 5%, P = 0.015). In particular, those subjects exposed to cooking fumes the previous evening (n = 28) revealed higher urinary mutagenicity (P = 0.035, positive urine 25% vs. 5%, P < 0.001) than non-indoor exposed. The sources of variability of the mutagenicity assay, mainly the histidine content of the urine concentrate (z = 4.06, P < 0.0001), and to a lesser extent bacterial inoculum size (z = 2.33, P = 0.019), also significantly influenced urinary mutagenicity values. In a linear multiple regression analysis, their effects were still significant (i.e., histidine content P = 0.026 and inoculum size P = 0.021), but the effects of diet, indoor exposure, and other environmental exposures (i.e., traffic and heating system exhausts, residential area) were not. It is concluded that the previous day's exposure to mutagen-rich meals and cooking fumes may influence the presence of mutagenic activity in the overnight urine of non-smoking subjects. This mutagenic activity, which remains in contact with bladder mucosa for several hours, could be considered risk factors for colorectal adenoma and possibly other cancers (i.e., bladder) in non-smokers. Accurate control of histidine content and bacterial inoculum size is strongly recommended when investigating the mutagenic activity of urine from non-smokers.

show abstract

Mutagenicity studies with urine concentrates from coke plant workers.

Cited by 36 publications

References 12 publications

Elevated urinary mutagenicity among those exposed to bituminous coal combustion emissions or diesel engine exhaust

Elevated urinary mutagenicity among those exposed to bituminous coal combustion emissions or diesel engine exhaust

Repeated Measurements of Urinary Methylated/Oxidative DNA Lesions, Acute Toxicity, and Mutagenicity in Coke Oven Workers

Mutagenic activity of overnight urine from healthy non‐smoking subjects

Contact Info

Product

Resources

About