1978
DOI: 10.1042/bst0060436
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Mutant Citrate Synthases from Acinetobacter Generated by Transformation

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Cited by 13 publications
(2 citation statements)
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“…Consistent with the absence of this enzyme was a growth requirement for glutamate, ooxoglutarate or proline. We have used such CS-mutants to obtain revertant CS' strains either spontaneously or after mutagenesis [lo] or by transformation [4]. The method of fluoroacetate resistance therefore does succeed in selecting CS-mutants and should, in principle, be applicable to a range of other organisms (both prokaryotic and eukaryotic) and should prove a useful additional approach complementing methods which have been applied by other investigators.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Consistent with the absence of this enzyme was a growth requirement for glutamate, ooxoglutarate or proline. We have used such CS-mutants to obtain revertant CS' strains either spontaneously or after mutagenesis [lo] or by transformation [4]. The method of fluoroacetate resistance therefore does succeed in selecting CS-mutants and should, in principle, be applicable to a range of other organisms (both prokaryotic and eukaryotic) and should prove a useful additional approach complementing methods which have been applied by other investigators.…”
Section: Resultsmentioning
confidence: 99%
“…Following our report on mutant citrate synthases from A. calcoaceticus [4], which referred to the isolation of CS-strains by selection for fluoroacetate resistance, we learned that J. T. Beatty and H. Gest (personal communication) have employed a similar procedure to isolate CS-mutants from Rhodopseudomonas capsulata.…”
Section: Resultsmentioning
confidence: 99%