1973
DOI: 10.1128/jb.116.2.619-626.1973
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Mutant of Escherichia coli K-12 Defective in the Transport of Basic Amino Acids

Abstract: Escherichia coli K-12 possesses two active transport systems for arginine, two for ornithine, and two for lysine. In each case there is a lowand a high-affinity transport system. They have been characterized kinetically and by response to competitive inhibition by arginine, lysine, ornithine and other structurally related amino acids. Competitors inhibit the high-affinity systems of the three amino acids, whereas the low-affinity systems are not inhibited. On the basis of kinetic evidence and competition studi… Show more

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Cited by 60 publications
(38 citation statements)
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“…3), which has not been previously reported in related species. The argT gene is predicted to encode the lysine/arginine/ornithine-binding protein, a periplasmic protein responsible for binding the amino acid substrates during the process of amino acid transport into the cytoplasm (29). According to studies in E. coli, the expression of ArgT is up-regulated in the stationary growth phase (30) or under glucose-limiting conditions (31), suggesting its role in the physiological adaptation of bacteria to detrimental conditions.…”
Section: Proteomics Analysis Of S Flexneri 2a 2457tmentioning
confidence: 99%
“…3), which has not been previously reported in related species. The argT gene is predicted to encode the lysine/arginine/ornithine-binding protein, a periplasmic protein responsible for binding the amino acid substrates during the process of amino acid transport into the cytoplasm (29). According to studies in E. coli, the expression of ArgT is up-regulated in the stationary growth phase (30) or under glucose-limiting conditions (31), suggesting its role in the physiological adaptation of bacteria to detrimental conditions.…”
Section: Proteomics Analysis Of S Flexneri 2a 2457tmentioning
confidence: 99%
“…The transport of the basic amino acids arginine, lysine, and ornithine is mediated by at least four physiologically distinct systems in Escherichia coli K-12. There are one common, high-affinity transport system for arginine, lysine, and ornithine (LAO system) and three low-affinity specific systems, one for each amino acid (8,33). Evidence for separate systems is based on biphasic kinetics of entry of all three basic amino acids and on competitive inhibitions at low, but not at high, substrate concentrations.…”
mentioning
confidence: 99%
“…The formation of the specific systems for arginine and ornithine is repressible either by arginine or ornithine, and the formation of the specific lysine system and the LAO system, by lysine (8).…”
mentioning
confidence: 99%
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“…Third, inhibition of UW85 growth in culture by both seed exudate and canavanine was prevented by adding arginine, histidine, or lysine (data not shown). These three amino acids are known to compete with canavanine for uptake into cells (11) and to overcome canavanine toxicity. Finally, UW2000, a spontaneous mutant of UW85 selected for resistance to commercially available canavanine, was resistant to alfalfa seed exudate and to our preparations of purified canavanine.…”
Section: Resultsmentioning
confidence: 99%