Mutant of Yeast Sensitive to 2,6-Diaminopurine

Abstract

A mutant of yeast sensitive to growth inhibition by 2,6-diaminopurine (2,6-DAP) was analyzed genetically and found to be a double mutant. One gene, dap, conferred approximately 30% sensitivity to the analogue. The other, slw, potentiated the inhibition such that the double mutant dap slw was inhibited 90%. The mutation dap conferred concomitant sensitivity to a number of other purine analogues. The activity of a purine phosphoribosyltransferase with 2, 6-DAP in a strain carrying dap was found to be three times higher than in the wild type. It is inferred that the mutation alters the properties of a purine phosphoribosyltransferase. A possible mechanism for the effect of slw is also discussed. Genetic methods. The techniques used for crossing and ascus dissection were those employed by Ahmed and Woods (1). Growth and inhibition studies. Cultures were grown at 28 C in 50-ml flasks containing 10 ml of YMM, with appropriate supplements, on a rotary shaker at 817

New Mutant Types at the ade3 Locus of Saccharomyces cerevisiae

“…Growth studies. Cultures were grown in 50-ml conical flasks on a rotary shaker as described previously (9). Yields (milligrams of dry weight per milliliter)…”