Mutants as a Genetic Resource for Future Crop Improvement

Gulfishan, Mohd; Bhat, Tariq Ahmad; Oves, Mohammad

doi:10.1007/978-3-319-22521-0_4

Cited by 7 publications

(2 citation statements)

References 70 publications

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“…Several sources of genetic variation are available to complement the genetic paucity of modern elite cultivars. Genetic diversity generated artificially through mutagenesis has proven a valuable resource for various crop species, as variants can be directly produced in commercial germplasm (Caldwell et al ., ; Mba, ; Nikam et al ., ; Gulfishan et al ., ; Çelik and Atak, ; Pando and Deza, ; Wang et al ., ; Tu Anh et al ., ). However, given that salt tolerance is most likely to arise from the concerted effects of numerous mechanisms, the potential to artificially create salt tolerant variants is surely limited.…”

Section: Harnessing the Genetic Diversity Of Exotic Germplasmmentioning

confidence: 99%

Salt stress under the scalpel – dissecting the genetics of salt tolerance

et al. 2019

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Summary Salt stress limits the productivity of crops grown under saline conditions, leading to substantial losses of yield in saline soils and under brackish and saline irrigation. Salt tolerant crops could alleviate these losses while both increasing irrigation opportunities and reducing agricultural demands on dwindling freshwater resources. However, despite significant efforts, progress towards this goal has been limited, largely because of the genetic complexity of salt tolerance for agronomically important yield‐related traits. Consequently, the focus is shifting to the study of traits that contribute to overall tolerance, thus breaking down salt tolerance into components that are more genetically tractable. Greater consideration of the plasticity of salt tolerance mechanisms throughout development and across environmental conditions furthers this dissection. The demand for more sophisticated and comprehensive methodologies is being met by parallel advances in high‐throughput phenotyping and sequencing technologies that are enabling the multivariate characterisation of vast germplasm resources. Alongside steady improvements in statistical genetics models, forward genetics approaches for elucidating salt tolerance mechanisms are gaining momentum. Subsequent quantitative trait locus and gene validation has also become more accessible, most recently through advanced techniques in molecular biology and genomic analysis, facilitating the translation of findings to the field. Besides fuelling the improvement of established crop species, this progress also facilitates the domestication of naturally salt tolerant orphan crops. Taken together, these advances herald a promising era of discovery for research into the genetics of salt tolerance in plants.

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Section: Harnessing the Genetic Diversity Of Exotic Germplasmmentioning

confidence: 99%

Salt stress under the scalpel – dissecting the genetics of salt tolerance

et al. 2019

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“…The development of new crop varieties with desirable agronomical traits, such as high productivity rates, has become crucial for this purpose. Since natural variation is scarce, breeding programs are in need of suitable alternatives and particularly induced mutagenesis programs [ 1 ]. Modern breeding programs should ideally be based on a combination of natural and induced mutagenesis supported by advanced molecular biology techniques [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

A Tomato EMS-Mutagenized Population Provides New Valuable Resources for Gene Discovery and Breeding of Developmental Traits

Fonseca

Capel

Nieto-Canseco

et al. 2022

Plants

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Tomato (Solanum lycopersicum L.) is a major horticultural crop and a model species among eudicots, especially for traits related to reproductive development. Although considerable progress has been made since the tomato genome sequence project was completed, most of the genes identified remain predictions with an unknown or hypothetical function. This lack of functional characterization hampers the use of the huge amount of genomic information available to improve the quality and productivity of this crop. Reverse genetics strategies such as artificial mutagenesis and next-generation sequencing approaches build the perfect tandem for increasing knowledge on functional annotation of tomato genes. This work reports the phenotypic characterization of a tomato mutant collection generated from an EMS chemical mutagenesis program aimed to identify interesting agronomic mutants and novel gene functions. Tomato mutants were grouped into fourteen phenotypic classes, including vegetative and reproductive development traits, and the inheritance pattern of the identified mutations was studied. In addition, causal mutation of a selected mutant line was isolated through a mapping-by-sequencing approach as a proof of concept of this strategy’s successful implementation. Results support tomato mutagenesis as an essential tool for functional genomics in this fleshy-fruited model species and a highly valuable resource for future breeding programs of this crop species aimed at the development of more productive and resilient new varieties under challenging climatic and production scenarios.

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