Mutants of Aspergillus nidulans blocked at an early stage of sporulation secrete an unusual metabolite

Butnick, N Z; Yager, L N; Hermann, Thomas; Kurtz, Myra B.; Champe, Sewell P.

doi:10.1128/jb.160.2.533-540.1984

Cited by 31 publications

(13 citation statements)

References 28 publications

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“…Another interesting developmental event occurring prior to brlA activation is the acquisition of developmental competence. Previ-ously, Champe's group used conditional mutations to show that three different aco (aconidial) genes, acoA, acoB, and acoC, are blocked in conidiation before the acquisition of developmental competence (29,30,33,170). These precompetence mutants were isolated based on the idea that genes required for competence may no longer be essential once a strain has already become competent.…”

Section: Activation Of the Central Regulatory Pathwaymentioning

confidence: 99%

Section: Activation Of the Central Regulatory Pathwaymentioning

confidence: 99%

“…acoA, acoB, and acoC mutants grown at the restrictive temperature all overproduce abundant quantities of a complex set of metabolites that absorb UV light (29,30). At least some of these compounds are diphenyl ethers, which probably arise from acetate through the polyketide pathway (29,35). Although acoA, acoB, and acoC mutants are sexually sterile at the restrictive temperature, they also produce large quantities of a hormone-like fatty acid metabolite called psi (precocious sexual inducer) factor that stimulates sexual sporulation while inhibiting asexual sporulation in wild-type strains (32,34,101).…”

Section: Activation Of the Central Regulatory Pathwaymentioning

confidence: 99%

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Asexual Sporulation inAspergillus nidulans

Adams

Wieser

1998

Microbiol Mol Biol Rev

652

402

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SUMMARY The formation of mitotically derived spores, called conidia, is a common reproductive mode in filamentous fungi, particularly among the large fungal class Ascomycetes. Asexual sporulation strategies are nearly as varied as fungal species; however, the formation of conidiophores, specialized multicellular reproductive structures, by the filamentous fungus Aspergillus nidulans has emerged as the leading model for understanding the mechanisms that control fungal sporulation. Initiation of A. nidulans conidipohore formation can occur either as a programmed event in the life cycle in response to intrinsic signals or to environmental stresses such as nutrient deprivation. In either case, a development-specific set of transcription factors is activated and these control the expression of each other as well as genes required for conidiophore morphogenesis. Recent progress has identified many of the earliest-acting genes needed for initiating conidiophore development and shown that there are at least two antagonistic signaling pathways that control this process. One pathway is modulated by a heterotrimeric G protein that when activated stimulates growth and represses both asexual and sexual sporulation as well as production of the toxic secondary metabolite, sterigmatocystin. The second pathway apparently requires an extracellular signal to induce sporulation-specific events and to direct the inactivation of the first pathway, removing developmental repression. A working model is presented in which the regulatory interactions between these two pathways during the fungal life cycle determine whether cells grow or develop.

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Section: Activation Of the Central Regulatory Pathwaymentioning

confidence: 99%

Section: Activation Of the Central Regulatory Pathwaymentioning

confidence: 99%

Section: Activation Of the Central Regulatory Pathwaymentioning

confidence: 99%

See 1 more Smart Citation

Asexual Sporulation inAspergillus nidulans

Adams

Wieser

1998

Microbiol Mol Biol Rev

652

402

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“…3b,c ). In contrast, features 14–16 were annotated as various adducts of diorcinol, an antibiotic secondary metabolite involved in fungal development 34 , and were collectively extracted best in chloroform ( Fig. 3c,e ).…”

Section: Resultsmentioning

confidence: 99%

Microbial metabolomics in open microscale platforms

et al. 2016

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The microbial secondary metabolome encompasses great synthetic diversity, empowering microbes to tune their chemical responses to changing microenvironments. Traditional metabolomics methods are ill-equipped to probe a wide variety of environments or environmental dynamics. Here we introduce a class of microscale culture platforms to analyse chemical diversity of fungal and bacterial secondary metabolomes. By leveraging stable biphasic interfaces to integrate microculture with small molecule isolation via liquid–liquid extraction, we enable metabolomics-scale analysis using mass spectrometry. This platform facilitates exploration of culture microenvironments (including rare media typically inaccessible using established methods), unusual organic solvents for metabolite isolation and microbial mutants. Utilizing Aspergillus, a fungal genus known for its rich secondary metabolism, we characterize the effects of culture geometry and growth matrix on secondary metabolism, highlighting the potential use of microscale systems to unlock unknown or cryptic secondary metabolites for natural products discovery. Finally, we demonstrate the potential for this class of microfluidic systems to study interkingdom communication between fungi and bacteria.

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“…In all cases, the conidial yields of the revertants grown at 42°C were comparable to that of the wild type, whereas cleistothecia were present in normal numbers for some isolates but completely absent for others ( Table 5). The revertant strains were analyzed by thin-layer chromatography for the presence of the set of metabolites character- a Parameters were measured as described previously (3,18).…”

Section: Resultsmentioning

confidence: 99%

Genetic analysis of mutants of Aspergillus nidulans blocked at an early stage of sporulation

Butnick¹,

Yager²,

Kurtz³

et al. 1984

J Bacteriol

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Three mutants of Aspergillus nidulans, selected to have a block at an early stage of conidiation (asexual sporulation), exhibit similar pleiotropic phenotypes. Each of these mutants, termed preinduction mutants, also are blocked in sexual sporulation and secrete a set of phenolic metabolites at level much higher than wild type or mutants blocked at later stages of conidiation. Backcrosses of these mutants to wild type showed that the three phenotypes always cosegregated. Diploids containing the mutant alleles in all pairwise combinations were normal for all phenotypes, showing that the three mutations are nonallelic. This conclusion was confirmed by the finding that the mutations map at three unlinked or distantly linked loci. Ten revertants of the two least leaky preinduction mutants, selected for ability to conidiate, were found in each case to arise by a second-site suppressor mutation. All of the revertants still showed accumulation of some of the phenolic metabolites but differed from each other in certain components. Three of the revertants retained the block in sexual sporulation. In these cases the suppressor has thus uncoupled the block in asexual sporulation from the block in sexual sporulation. These results are understandable in terms of a model in which preinduction mutations and their suppressors affect steps in a single metabolic pathway whose intermediates include an effector specific for asexual sporulation and a second effector specific for sexual sporulation.

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Mutants of Aspergillus nidulans blocked at an early stage of sporulation secrete an unusual metabolite

Cited by 31 publications

References 28 publications

Asexual Sporulation inAspergillus nidulans

Asexual Sporulation inAspergillus nidulans

Microbial metabolomics in open microscale platforms

Genetic analysis of mutants of Aspergillus nidulans blocked at an early stage of sporulation

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