1992
DOI: 10.1111/j.1432-1033.1992.tb16804.x
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Mutants of human insulin‐like growth factor II

Abstract: Insulin-like growth factor I1 (IGF 11) and four structural analogs, constructed by site-directed mutagenesis, were expressed as protein A fusion proteins in Escherichia coli BL2lpLysS cells, cleaved with cyanogen bromide and purified by affinity chromatography and HPLC. Two mutants (Ser29 substituted by Arg-Leu-Pro-Gly, and Ser33 substituted by Cys-Gly-Asp) represent two naturally occurring variants of IGF 11. The other two mutants, (7 -67)IGF I1 and (9 -67)IGF 11, are truncated at the amino-terminus in analo… Show more

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Cited by 25 publications
(14 citation statements)
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“…Identical results were obtained in an assay with IGF-I1 species isolated from a separate DAP-I digestion. This result is in essential agreement with recent reports that des-1-6-IGF-I1 exhibited a normal binding affinity for the human IGF-I receptor overexpressed in NIH-3T3 cells and 56% of the binding affinity of IGF-I1 for toe IGF-I receptor in rat L6 myoblasts (Luthi et al 1992;Francis 1993). We assessed the ability of IGF-I1 and des-Ala-nr-IGF-I1 to stimulate DNA synthesis via the IGF-I receptor in MG-63 human osteosarcoma cells (Fig.…”
Section: Biological Activity Of Dap-i Cleavage Product Of Igf-11supporting
confidence: 93%
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“…Identical results were obtained in an assay with IGF-I1 species isolated from a separate DAP-I digestion. This result is in essential agreement with recent reports that des-1-6-IGF-I1 exhibited a normal binding affinity for the human IGF-I receptor overexpressed in NIH-3T3 cells and 56% of the binding affinity of IGF-I1 for toe IGF-I receptor in rat L6 myoblasts (Luthi et al 1992;Francis 1993). We assessed the ability of IGF-I1 and des-Ala-nr-IGF-I1 to stimulate DNA synthesis via the IGF-I receptor in MG-63 human osteosarcoma cells (Fig.…”
Section: Biological Activity Of Dap-i Cleavage Product Of Igf-11supporting
confidence: 93%
“…This result would be predicted from the recent reports that des-1-6-IGF-I1 and IGF-I1 were essentially equipotent in stimulating DNA synthesis in NIH-3T3 cells overexpressing the human IGF-I receptor and in stimulating protein synthesis in L6 myoblasts (Luthi et al, 1992;Francis et al, 1993). The native and truncated IGF-I1 species were also compared for their ability to bind to two preparations of IGFbinding proteins.…”
Section: Biological Activity Of Dap-i Cleavage Product Of Igf-11mentioning
confidence: 79%
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“…Several intermolecular NOE connectivities were observed from these residues to the proton resonances of the aromatic side‐chains of IGF‐II (Figure 8). Site‐directed mutagenesis studies on IGFs indicate that IGF residues Glu6, Phe48, Arg49 and Ser50 are important for binding to IGFBPs (Luthi et al ., 1992; Bach et al ., 1993; Francis et al ., 1993; Jansson et al ., 1997). It was suggested also that Phe26 of IGFs plays a role in changing the local structures of IGFs but does not bind directly to IGFBPs (Terasawa et al ., 1994).…”
Section: Discussionmentioning
confidence: 99%
“…The complex of IGF-IGFBPs has a relatively long half-life and is present in the intravascular space [2,3]. N-terminal truncated variants of IGF-I and IGF-II, des (1-3) IGF-I [4,5] and des (1-6) IGF-II [6], are biologically more potent than intact IGF-I and IGF-II, respectively, because these variants have reduced affinity for the IGFBPs but their affinity for the type-I IGF receptor is similar to that of intact IGF. Des (1-3) IGF-I has been identified in a variety of tissue extracts and biological fluids [7][8][9][10], whereas des (1-6) IGF-II has not yet been found.…”
mentioning
confidence: 99%