Mutation Analysis of Braf Exon 15 and Kras Codons 12 and 13 in Moroccan Patients with Colorectal Cancer

Bennani, Bahia; Gilles, Sophie; Fina, Frédéric; Nanni, Isabelle; Ibrahimi, Sidi Adil; Riffi, Afaf Amarti; Nejjari, Chakib; Benajeh, D.; Abkari, Mohammed El; Martin, Pierre Marie; Ouafik, L’Houcine

doi:10.5301/jbm.2010.6091

Cited by 13 publications

(23 citation statements)

References 31 publications

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“…A total 14 studies1314151617181920212223242526 with 1324 samples were included in our study and the average sample number was 94.5 (range 13 to 201). The earliest study was in March 200515, while the latest was in April 20132526.…”

Section: Resultsmentioning

confidence: 99%

“…Instrument types included HR-1 (n = 2), LightCycler480 (n = 8), RotorGene6000 (n = 3) and Rotor-Gene Q (n = 1). The dye included LCGreen(n = 1), Syto 9(n = 5), LightCycler LC480 High Resolution Melting Master(n = 6),EvaGreen(n = 1),and one study didn't mention about it17. The final volumes (μL) of HRM were 10 μL (n = 8), 20 μL (n = 4), 25 μL (n = 1), while one study didn't mention about it19.…”

Section: Resultsmentioning

confidence: 99%

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High-Resolution Melting Analysis for accurate detection of BRAF mutations: a systematic review and meta-analysis

Chen

Wang

Chuai

et al. 2014

Sci Rep

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The high-resolution melting curve analysis (HRMA) might be a good alternative method for rapid detection of BRAF mutations. However, the accuracy of HRMA in detection of BRAF mutations has not been systematically evaluated. We performed a systematic review and meta-analysis involving 1324 samples from 14 separate studies. The overall sensitivity of HRMA was 0.99 (95% confidence interval (CI) = 0.75–0.82), and the overall specificity was very high at 0.99 (95% CI = 0.94–0.98). The values for the pooled positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio were 68.01 (95% CI = 25.33–182.64), 0.06 (95% CI = 0.03–0.11), and1263.76 (95% CI = 393.91–4064.39), respectively. The summary receiver operating characteristic curve for the same data shows an area of 1.00 and a Q* value of 0.97. The high sensitivity and specificity, simplicity, low cost, less labor or time and rapid turnaround make HRMA a good alternative method for rapid detection of BRAF mutations in the clinical practice.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

High-Resolution Melting Analysis for accurate detection of BRAF mutations: a systematic review and meta-analysis

Chen

Wang

Chuai

et al. 2014

Sci Rep

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“…Fifteen full-text papers were excluded for the reasons described in Figure 1 . Finally, 13 studies 5 10 15 16 17 18 19 20 21 22 23 24 25 met the inclusion criteria and were included in this meta-analysis. These articles were divided into 15 ‘units' for statistical analysis according to the specimen source.…”

Section: Resultsmentioning

confidence: 99%

“…However, there are discordant results between HRM and DNA sequencing in seven 5 10 19 20 21 22 23 of the 13 included studies. The total number of false positives was 44, which may be explained by different limits of detection or sensitivity for the two methods.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic accuracy of high resolution melting analysis for detection of KRAS mutations: a systematic review and meta-analysis

Liu

Xiang

et al. 2014

Sci Rep

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Increasing evidence points to a negative correlation between KRAS mutations and patients' responses to anti-EGFR monoclonal antibody treatment. Therefore, patients must undergo KRAS mutation detection to be eligible for treatment. High resolution melting analysis (HRM) is gaining increasing attention in KRAS mutation detection. However, its accuracy has not been systematically evaluated. We conducted a meta-analysis of published articles, involving 13 articles with 1,520 samples, to assess its diagnostic accuracy compared with DNA sequencing. The quality of included articles was assessed using the revised Quality Assessment for Studies of Diagnostic Accuracy (QUADAS-2) tools. Random effects models were applied to analyze the performance of pooled characteristics. The overall sensitivity and specificity of HRM were 0.99 (95% confidence interval [CI]: 0.98–1.00) and 0.96 (95%CI: 0.94–0.97), respectively. The area under the summary receiver operating characteristic curve was 0.996. High sensitivity and specificity, less labor, rapid turn-around and the closed-tube format of HRM make it an attractive choice for rapid detection of KRAS mutations in clinical practice. The burden of DNA sequencing can be reduced dramatically by the implementation of HRM, but positive results still need to be sequenced for diagnostic confirmation.

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“…This result confirms an earlier study led in Hassan II University Hospital of Fez, which took 62 samples of patients with sporadic colorectal adenocarcinomas brought in our pathology laboratory before 2010. So, only one patient (1.6 %) carried BRAF codon 600 mutation [ 24 ], that is lower than what found in literature, were BRAF mutations are found out in approximately 10–15 % of CRC. In Tunisia, where population is similar to Morocco, one study, including 51 patients with CRC, found out also just one patient with a BRAF somatic mutation and this tumor has a MSS status [ 25 ].…”

Section: Discussionmentioning

confidence: 96%

Epigenetics could explain some Moroccan population colorectal cancers peculiarities: microsatellite instability pathway exploration

et al. 2015

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BackgroundColorectal Cancers (CRC) are one of the most common malignancies in the world. Their incidence in Morocco, between 2005 and 2007, was 5.6 for 100000 inhabitants, which is very low compared to what found in developed countries. In addition, CRCs show a high frequency of rectal localizations, and occurs in a younger population in Morocco compared to what found in developed countries.The purpose of this study is to confirm these CRC peculiarities in Morocco and try to explain them by exploring the microsatellite instability molecular pathway.MethodsThis is a prospective observational study conducted since January 2010, including 385 patients admitted in Hassan II University Hospital of Fez. We collected clinical, radiological and pathological data. We investigated the expression of mismatch repair (MMR) proteins in 214 patients and BRAF gene mutations in 159 patients.ResultsMean age was 55.08 +/− 15.16 years. 36.5 % of patients were less than 50 years old and 49.3 % of tumors were localized in the rectum. Loss of MMR protein expression was observed in 11.2 % of cases. It was independently associated with individual or family history of cancer belonging to Hereditary Non-Polyposis Colorectal Cancer (HNPCC) spectrum (p = 0.01) and proximal localization (p = 0.02). No BRAF mutation was detected in all cases.ConclusionsThese results confirm the high occurrence of CRCs to young patients and the high frequency of rectal localizations in Moroccan population. They mostly show an absence of BRAF mutation, supposing a rarity of MLH1 promoter hypermethylation pathway, which may even partially explain the CRC peculiarities in our context.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/5868184711716884

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Mutation Analysis of Braf Exon 15 and Kras Codons 12 and 13 in Moroccan Patients with Colorectal Cancer

Cited by 13 publications

References 31 publications

High-Resolution Melting Analysis for accurate detection of BRAF mutations: a systematic review and meta-analysis

High-Resolution Melting Analysis for accurate detection of BRAF mutations: a systematic review and meta-analysis

Diagnostic accuracy of high resolution melting analysis for detection of KRAS mutations: a systematic review and meta-analysis

Epigenetics could explain some Moroccan population colorectal cancers peculiarities: microsatellite instability pathway exploration

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