Mutation and selection for improved oil and meal quality in Brassica napus utilizing microspore culture

Kott, L. S.; Wong, R. S. C.; Swanson, E. B.; Chen, J.

doi:10.1007/978-94-017-0477-9_7

Cited by 18 publications

(10 citation statements)

References 34 publications

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“…The advantage of this system is that any genetic variation introduced will permit recovery of diploid mutant/transgenic plants that are homozygous for mutant/~ans-genes after spontaneous or chemical chromosome doubling (Kott et al, 1996;Yao et al, 1997). Recently, the topic of "microspore-based transformation" has been reviewed by Touraev et al (2001).…”

Section: Biotechnoloby Potentialmentioning

confidence: 99%

The canola microspore-derived embryo as a model system to study developmental processes in plants

Yeung

2002

J. Plant Biol.

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The ability of micrmpores to undergo embryo development after a successful induction treatment provides a unique experimental system to study a variety of developmental processes in plants. Recent published results focus on the cellular and molecular aspects of the early induction process. In this review, besides summarizing the current findings, the advantages of using the MDE system to study other aspects of embryo development are emphasized. The continual improvement of culturing procedures, media components, and molecular methods guarantees exciting new findings in the near future. Keywords: Brassica, embryo development, microspore-derived embryosThe reports by Maheshwari in 1964 and1966 demonstrated that microspores of a flowering plant, Datura, are capable of developing into haploid plants. This discovery clearly illustrates the added developmental potential of microspores as they can switch from gametophytic to sporophytic development under appropriate conditions. As a result, this observation has enabled further development of haploid plant technology. Since the original reports by Guha and Maheshwari, successes have been reported for different species (see Jain et al., 1996-t997). A majority of studies have been focused on tobacco, Brassica species, and different varieties of barley, rice and wheat. This is primarily due to their economic importance or their ease of manipulation. Although a large amount of information is available in the literature, emphasis is usually placed on the pre-culturing conditions, the stress treatments, the media components and the early cellular, biochemical and molecular events related to the induction process. The purpose of this review is to summarize recent findings and to draw attention to the usefulness of the rnicrospore-derived embryo (MDE) system in the study of other processes related to embryo development and maturation. Since not every aspect of MDE development will be discussed, readers are urged to consult reviews by Ferrie et al.

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Section: Biotechnoloby Potentialmentioning

confidence: 99%

The canola microspore-derived embryo as a model system to study developmental processes in plants

Yeung

2002

J. Plant Biol.

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“…1) (Kunst et al 1992;James et al 1995;Nath 2008). The development of seed oils bearing a high percentage of erucic acid for industrial applications is a subject of research (Taylor et al 1995;Kott et al 1996) because it is a very excellent renewable raw material used in plastic film manufacture, in the synthesis of nylon and in the lubricant and emollient Rapeseed Oils Food, Industry and Biodiesel • 127 industries (Leonard 1994;Sonntag 1995;Murphy 1996). A rapeseed line containing high proportions of erucic acid would significantly reduce the processing costs of itself for using as the industrial feed-stock.…”

Section: Development Of Vlcfa Rapeseed Linesmentioning

confidence: 99%

Modification of Fatty Acid Profiles of Rapeseed (Brassica napus L.) Oil for Using as Food, Industrial Feed-Stock and Biodiesel

Nath¹,

Kim²,

Khatun³

et al. 2016

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Rapeseed is a member of family Brassicaceae, cultivated as oil crop. Rapeseed oil is being utilized from early civilization, but its popularity being declined from the mid-nineteenth century due to presence of erucic acid (C22:1) and glucosinolates. Thereby, several attempts have been made to develop cultivars free from those toxins. In the past 20 years, breeders got success in developing '00'-quality rapeseed, known as 'Canola'. The target mutagenesis of fae-1 and fae-2 of Brassica napus ensured such success. Thereafter, 'canola' regains its market as a healthy vegetable oil. Moreover, high oleic acid rapeseed lines, with 86% oleic acid, have been developed by using chemical mutagenesis of FAD2 alleles responsible for desaturation of oleic acid (C18:1) to linoleic acid (C18:2). Recently, high erucic acid rapeseed oil regained interest for biodegradable plastic, cosmetic, emollient industries and for biodiesel. Therefore, breeding approaches have been pursued; unfortunately, that were failed to reach erucic acid level beyond 50% in seed-oil. Rapeseed genotypes over-expressed with Ld-LPAAT separately and Ld-LPAAT-FAE chimaric construct together were tried but failed to reach the erucic acid content more than 60%. Thereof, combined effort of conventional breeding and transgenic approaches are brought together to overcome three hypothesized bottlenecks; reviewed in this article, which restricted erucic acid level near to 60%. Finally, rapeseed genotypes with 78% erucic acid were developed successfully. This material is now available in Germany for using in emollient industries and for biodiesel. Therefore, this article is reviewed on the current status and future outlook for modification of fatty acid profiles of rapeseed oil for its end-use as food, industrial feed-stock and biodiesel.

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“…Consequently, much of our knowledge of genetics of higher organisms is based upon works utilizing induced mutations for analyzing gene function (McCallum et al, 2000). To date, several welldocumented examples of successful applications of mutation breeding to oilseed crops have been reported in the literature Bacelis, 2001;Bhatia et al, 1999;Ferrie et al, 2008;Fowler and Stefansson, 1972;Kott et al, 1996;MacDonald et al, 1991;Newsholme et al, 1989;Osorio et al, 1995;Parry et al, 2009;Rowland, 1991;Sala et al, 2008;Schnurbush et al, 2000;Spasibionek, 2006;Swanson et al, 1989;Velasco et al, 2008). Induced mutations have been used mainly to generate variation that could rarely be found in germplasm collections.…”

Section: Introductionmentioning

confidence: 99%

“…In winter canola, chemical mutagenesis was used to isolate two canola mutants of the cultivar "Winfield" with high oleic acid content (Wong and Swanson, 1991). Mutation breeding in canola has been also used to improve herbicide resistance Sala et al, 2008;Swanson et al, 1988Swanson et al, , 1989, disease resistance MacDonald et al, 1991;MacDonald and Ingram, 1986;Newsholme et al, 1989), and lower glucosinolate content (Barro et al, 2002;Kott, 1998;Kott et al, 1996). Chemical and physical mutagens are available for mutagenic treatment of crop plants.…”

Section: Introductionmentioning

confidence: 99%

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

Emrani¹,

Arzani²,

Saeidi³

2011

Afr. J. Biotechnol.

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Mutation induction is considered as an effective way to enrich plant genetic variation, particularly for traits with a very low level of genetic variation. The objectives of this study were to evaluate the effect of different dosages of chemical mutagens on seed germination, seed viability and seedling growth characteristics and to identify optimum treatment conditions for chemical mutagens based on the LD 50 criterion in canola (Brassica napus L.). Two pretreatment conditions of soaking in distilled water and non-soaking, different concentrations of chemical mutagens, and four treatment periods were investigated. The effect of mutagen dosage on seed viability was also assessed using the tetrazolium staining test. Results revealed the significant effects of mutagen dosages and treatment periods on seed viability and seed germination as well as on seedling characteristics for all the mutagens tested. Additionally, it was found that increased dosage and period in each treatment led to significant reductions in seed viability for the tested mutagens. Pretreatment did not significantly influence most of the studied characteristics. The 0.8% ethyl methanesulfonate (EMS) for 6 h, 12 mM N-nitroso-Nethylurea (ENU) and 6 mM sodium azide for 8 h and 9 mM N-nitroso-N-methylurea (NMU) for 4 h were considered as optimum treatment conditions.

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Mutation and selection for improved oil and meal quality in Brassica napus utilizing microspore culture

Cited by 18 publications

References 34 publications

The canola microspore-derived embryo as a model system to study developmental processes in plants

The canola microspore-derived embryo as a model system to study developmental processes in plants

Modification of Fatty Acid Profiles of Rapeseed (Brassica napus L.) Oil for Using as Food, Industrial Feed-Stock and Biodiesel

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

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