Mutation detection in the promoter region of survivin gene on N-methyl-N-nitrosourea induced colon tumor model in experiment

Budak, Metin; Ma, Korpinar; Kalkan, Tunaya; Tuncel, Handan

doi:10.4149/bll_2014_107

Cited by 2 publications

(2 citation statements)

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“…Matrix metalloproteinase-2 (MMP-2) is the most widely distributed member of the MMP family, and is able to degrade the extracellular matrix (ECM) and basement membrane, thus participating in physiological and pathological processes including tumor progression (8,9). Survivin is an important member of the inhibitors of apoptosis proteins gene family, and is closely associated with the differentiation, proliferation, angiogenesis, invasion and metastasis of tumor cells (10,11). In the present study, indomethacin, a cyclooxygenase inhibitor, was used in combination with oxaliplatin, a chemotherapeutic drug, to perform intervention treatment on lung cancer-nude mouse transplanted tumors.…”

Section: Introductionmentioning

confidence: 99%

Impact and mechanism of non-steroidal anti-inflammatory drugs combined with chemotherapeutic drugs on human lung cancer-nude mouse transplanted tumors

Sun

Chen

2016

Oncology Letters

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Abstract. The present study aimed to investigate the impact of indomethacin treatment combined with oxaliplatin treatment on the expression of cluster of differentiation 44 variant 6 (CD44v6), matrix metalloproteinase-2 (MMP-2) and survivin in human lung cancer-nude mouse transplanted tumors. The human lung adenocarcinoma (A549)-nude mouse transplanted tumor model was established, and the mice were divided into a control group, an indomethacin treatment group, an oxaliplatin treatment group and an indomethacin-oxaliplatin combination treatment group. The tumor inhibition rate was calculated following sacrificing of the mice. Immunohistochemical staining and fluorescence reverse transcription-quantitative polymerase chain reaction were utilized to detect the protein and messenger (m)RNA expression of CD44v6, MMP-2 and survivin. The tumor inhibition rates of the indomethacin group, the oxaliplatin group and the combination group were 26.67, 47.70 and 68.88%, respectively. The protein and mRNA expression levels of CD44v6, MMP-2 and survivin in the transplanted tumors of each treatment group were reduced compared with the control group (P<0.05), and those of the combination group were lower compared with the single-drug treatment groups (P<0.05). Survivin and MMP-2, MMP-2 and CD44v6, and MMP-2 and CD44v6 all exhibited linear positive correlation. The present study provides evidence that the administration of indomethacin alone, or in combination with oxaliplatin, may significantly inhibit the growth of lung cancer-nude mouse transplanted tumors and the expression of CD44v6, MMP-2 and survivin inside the tumor. The combination of non-steroidal anti-inflammatory drugs with chemotherapeutic drugs may improve the antitumor effects.

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Section: Introductionmentioning

confidence: 99%

Impact and mechanism of non-steroidal anti-inflammatory drugs combined with chemotherapeutic drugs on human lung cancer-nude mouse transplanted tumors

Sun

Chen

2016

Oncology Letters

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“…The main reason of this is the fact that most treatments exert their effects by activating apoptotic mechanisms. Targeting the apoptotic pathway ensures the effectiveness of anti-cancer treatments[41,42].…”

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confidence: 99%

Epigenetic Modifications and Potential Treatment Approaches in Lung Cancers

Budak¹,

Yıldız²

2018

Lung Cancer - Strategies for Diagnosis and Treatment

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Alteration of methylation is a process seen across a wide variety of species ranging from bacterial microorganisms to mammals, defined as the adaptation method the organism develops against environmental or intrinsic effects, or employs to switch off the genome regions which are no longer required through the evolutionary process. Scientific advancements have allowed detecting the regions that undergo different patterns of methylation. It has been demonstrated that the control on changes in gene expression is not guided by transcription factors alone and that epigenetic alterations are also involved in this process. Furthermore, epigenetic modifications have been shown to be considerably important in cancer development. This section focuses on epigenetic changes and potential treatment options in lung cancer.

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Mutation detection in the promoter region of survivin gene on N-methyl-N-nitrosourea induced colon tumor model in experiment

Cited by 2 publications

References 11 publications

Impact and mechanism of non-steroidal anti-inflammatory drugs combined with chemotherapeutic drugs on human lung cancer-nude mouse transplanted tumors

Impact and mechanism of non-steroidal anti-inflammatory drugs combined with chemotherapeutic drugs on human lung cancer-nude mouse transplanted tumors

Epigenetic Modifications and Potential Treatment Approaches in Lung Cancers

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