Mutation in <i>OsCADT1</i> enhances cadmium tolerance and enriches selenium in rice grain

Chen, Jie; Huang, Xin‐Yuan; Salt, David E.; Zhao, Fang‐Jie

doi:10.1111/nph.16404

Cited by 64 publications

(29 citation statements)

References 53 publications

(67 reference statements)

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“…The multiple localization of AtNAA50-EYFP in the cytosol and the ER has been independently confirmed by Neubauer and Innes (2020). The authors want to put a note of caution on the nuclear localization of AtNAA50-EYFP, since EYFP on its own is known to display nucleocytoplasmic localization (Chen et al, 2020). Hence, we cannot exclude that EYFP contributes to the nucleocytoplasmic localization of AtNAA50-EYFP.…”

Section: Depletion Of Naa50 Leads To a Severe Growth Retardationmentioning

confidence: 91%

NAA50 Is an Enzymatically Active N^α-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses

et al. 2020

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N a-terminal acetylation (NTA) is a prevalent protein modification in eukaryotes. In plants, the biological function of NTA remains enigmatic. The dominant N-acetyltransferase (Nat) in Arabidopsis (Arabidopsis thaliana) is NatA, which cotranslationally catalyzes acetylation of ;40% of the proteome. The core NatA complex consists of the catalytic subunit NAA10 and the ribosome-anchoring subunit NAA15. In human (Homo sapiens), fruit fly (Drosophila melanogaster), and yeast (Saccharomyces cerevisiae), this core NatA complex interacts with NAA50 to form the NatE complex. While in metazoa, NAA50 has N-acetyltransferase activity, yeast NAA50 is catalytically inactive and positions NatA at the ribosome tunnel exit. Here, we report the identification and characterization of Arabidopsis NAA50 (AT5G11340). Consistent with its putative function as a cotranslationally acting Nat, AtNAA50-EYFP localized to the cytosol and the endoplasmic reticulum but also to the nuclei. We demonstrate that purified AtNAA50 displays N a-terminal acetyltransferase and lysine-«-autoacetyltransferase activity in vitro. Global N-acetylome profiling of Escherichia coli cells expressing AtNAA50 revealed conservation of NatE substrate specificity between plants and humans. Unlike the embryo-lethal phenotype caused by the absence of AtNAA10 and AtNAA15, loss of NAA50 expression resulted in severe growth retardation and infertility in two Arabidopsis transfer DNA insertion lines (naa50-1 and naa50-2). The phenotype of naa50-2 was rescued by the expression of HsNAA50 or AtNAA50. In contrast, the inactive ScNAA50 failed to complement naa50-2. Remarkably, loss of NAA50 expression did not affect NTA of known NatA substrates and caused the accumulation of proteins involved in stress responses. Overall, our results emphasize a relevant role of AtNAA50 in plant defense and development, which is independent of the essential NatA activity.

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Section: Depletion Of Naa50 Leads To a Severe Growth Retardationmentioning

confidence: 91%

NAA50 Is an Enzymatically Active N^α-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses

et al. 2020

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“…Chromosomes were counted as previously described (30). All plants were cultured in 0.5× Kimura B nutrient solution (54) in a growth chamber with 28°C/25°C (day/night) and 12 h/12 h (light/ dark) cycles. Seedlings at the trefoil stage were treated by a series of 0, 50, 100, 150, and 200 mM NaCl for 6 d and recovered in the nutrient solution for 5 d to determine optimal concentrations.…”

Section: Methodsmentioning

confidence: 99%

DNA hypomethylation in tetraploid rice potentiates stress-responsive gene expression for salt tolerance

Wang

Cao

Wang

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Polyploidy is a prominent feature for genome evolution in many animals and all flowering plants. Plant polyploids often show enhanced fitness in diverse and extreme environments, but the molecular basis for this remains elusive. Soil salinity presents challenges for many plants including agricultural crops. Here we report that salt tolerance is enhanced in tetraploid rice through lower sodium uptake and correlates with epigenetic regulation of jasmonic acid (JA)–related genes. Polyploidy induces DNA hypomethylation and potentiates genomic loci coexistent with many stress-responsive genes, which are generally associated with proximal transposable elements (TEs). Under salt stress, the stress-responsive genes including those in the JA pathway are more rapidly induced and expressed at higher levels in tetraploid than in diploid rice, which is concurrent with increased jasmonoyl isoleucine (JA-Ile) content and JA signaling to confer stress tolerance. After stress, elevated expression of stress-responsive genes in tetraploid rice can induce hypermethylation and suppression of the TEs adjacent to stress-responsive genes. These induced responses are reproducible in a recurring round of salt stress and shared between two japonica tetraploid rice lines. The data collectively suggest a feedback relationship between polyploidy-induced hypomethylation in rapid and strong stress response and stress-induced hypermethylation to repress proximal TEs and/or TE-associated stress-responsive genes. This feedback regulation may provide a molecular basis for selection to enhance adaptation of polyploid plants and crops during evolution and domestication.

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“…Rice ( Oryza sativa L.) is one of the most important cereal crops globally, and about half of the world’s population depends on it for food. Compared to other cereals, rice is considered an efficient Cd accumulator due to its ability to absorb Cd from the soil and further translocate into the grain [ 6 ]. It is a major dietary source of Cd [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Forms of Selenium on the Physiological Response and the Cadmium Uptake by Rice under Cadmium Stress

Yan

Qin

et al. 2020

IJERPH

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Cadmium (Cd) is a pollutant toxic to plants and a potential threat to human health. Selenium (Se), though not essential for plants, has beneficial effects on plants under abiotic stress. A hydroponic experiment was conducted to investigate the impact of different forms of Se (Nano-Se, selenite, selenate, and SeMet) on accumulation, subcellular distribution, and chemical forms of Cd, as well as oxidative stress in rice seedlings. Cd (20 μmol·L−1) treatment significantly decreased biomass accumulation and chlorophyll content. The application of all Se forms, except selenate, mitigated the adverse effects of Cd on growth and chlorophyll content. The application of selenite, Nano-Se, and SeMet decreased root and shoot Cd concentrations as well as root-to-shoot Cd translocation in rice seedlings. Selenate application decreased shoot Cd concentration and root-to-shoot Cd translocation with no effect on root Cd concentration. Accordingly, Se increased the sequestration of Cd in the cell wall and vacuoles and decreased the active chemical form of Cd in rice seedlings. SeMet was the most effective supplement that decreased Cd concentration and enhanced Se concentration in the roots and shoots of rice seedlings. All forms of Se further enhanced catalase (CAT) and glutathione peroxidase (GSH-Px) activities and inhibited MDA accumulation. To conclude, Se influenced Cd accumulation and translocation in rice seedlings by altering the subcellular distribution, chemical forms, and antioxidant defense system under Cd stress. These effects were highly significant with SeMet treatment, probably due to better absorption and utilization by the plant.

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Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain

Cited by 64 publications

References 53 publications

NAA50 Is an Enzymatically Active N^α-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses

NAA50 Is an Enzymatically Active N^α-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses

DNA hypomethylation in tetraploid rice potentiates stress-responsive gene expression for salt tolerance

Effect of Different Forms of Selenium on the Physiological Response and the Cadmium Uptake by Rice under Cadmium Stress

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Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain

Cited by 64 publications

References 53 publications

NAA50 Is an Enzymatically Active Nα-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses

NAA50 Is an Enzymatically Active Nα-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses

DNA hypomethylation in tetraploid rice potentiates stress-responsive gene expression for salt tolerance

Effect of Different Forms of Selenium on the Physiological Response and the Cadmium Uptake by Rice under Cadmium Stress

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NAA50 Is an Enzymatically Active N^α-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses

NAA50 Is an Enzymatically Active N^α-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses