Mutation Induction in Vegetative and Developing Cells of Dictyostelium Discoideum

Deering, R. A.; Sheely, Mary

doi:10.1016/b978-0-444-41608-7.50012-5

Cited by 6 publications

(2 citation statements)

References 6 publications

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“…Mutation frequency. The procedures were in principle the same as those previously described (Deering and Sheely, 1977;Ohnishi et al, 1982). After germination, the expression of mutation was achieved by incubating in suspension for 36 h with NC4 or 48 h with gs13.…”

Section: Methodsmentioning

confidence: 99%

Mutation frequency of Dictyostelium discoideum spores exposed to the space environment.

Takahashi

Ohnishi

Fukui³

et al. 1997

Biol. Sci. Space

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Two strains of cellular slime mold Dictyostelium discoideum, a radiosensitive mutant and the parental wild-type strain, were used to investigate the effects of cosmic radiation on viability and mutation frequency at the spore stage for about 9 days in Space Shuttle of NASA. We measured little effect of space environment on viability and cell growth in the both strains as compared to ground controls. The mutation frequency of the flown spores were similar to that of ground control. These results suggest that there could be no effect of cosmic radiation, containing high linear energy transfer radiation at about 0.9 mSv/day as detected by real-time radiation monitoring device on the induction of mutation at the spore stage.

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Section: Methodsmentioning

confidence: 99%

Mutation frequency of Dictyostelium discoideum spores exposed to the space environment.

Takahashi

Ohnishi

Fukui³

et al. 1997

Biol. Sci. Space

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“…Induced mutation frequency. The procedures were in principle the same as those previously described (Deering and Sheely, 1977;Ohnishi et al, 1982). After irradiation by HL light, the amoeboid cells were transferred into a P,-buffer with E. coli cells at 1Olo cellslmC.…”

Section: Wave Length (Nm)mentioning

confidence: 99%

DNA DAMAGE and ITS REPAIR IN Dictyostelium discoideum IRRADIATED BY HEALTH LAMP LIGHT (UV‐ B)

Okaichi¹,

Kajitani²,

Nakajima

et al. 1989

Photochem & Photobiology

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Abstract— Irradiation by health lamp (HL) light (280–320 nm) more efficiently induced cell killing and mutation in a radiation sensitive mutant (TW8) of Dictyostelium discoideum as compared with the parental wild‐type strain (NC4). This light as well as a germicidal lamp‐light (254 nm) produced pyrimidine dimers. The dimers were removed from DNA molecules by excision repair in NC4, but more slowly in TW8. It is suggested that pyrimidine dimers are the main DNA damage caused by HL light in D. discoideum, and that this results in cell killing and induced mutation.

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In vivo nicking and rejoining of nuclear DNA in ultraviolet-irradiated radiation-resistant and sensitive strains of Dictyostelium discoideum

Welker

Deering

1979

Molec. Gen. Genet.

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Some aspects of DNA repair in several radiation-resistant and radiation-sensitive strains of Dictyostelium discoideum were investigated by using alkaline sucrose gradients to analyze for the production and resealing of single-strand breaks following irradiation with 254 nm UV. All radiation-resistant strains and all mutants assayed that are sensitive to both UV and 60Co gamma rays produced single-strand breaks in their nuclear DNA after a UV fluence of 15 M/m2. Mutants at the radC locus which are sensitive to UV but as resistant as their parental strains to 60Co gamma rays produced many fewer single-strand breaks in their DNA after irradiation with UV. Thus, the radC mutations alter a repair pathway specific for UV-induced DNA damage and presumably affect the activity of a UV-damage-specific endonuclease involved in excision repair. All radiation-resistant strains and all of our mutants sensitive to gamma rays rejoined much of their DNA during a three-hour post-UV-irradiation incubation, suggesting that these strains have at least a partially intact excision repair system.

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Mutation Induction in Vegetative and Developing Cells of Dictyostelium Discoideum

Cited by 6 publications

References 6 publications

Mutation frequency of Dictyostelium discoideum spores exposed to the space environment.

Mutation frequency of Dictyostelium discoideum spores exposed to the space environment.

DNA DAMAGE and ITS REPAIR IN Dictyostelium discoideum IRRADIATED BY HEALTH LAMP LIGHT (UV‐ B)

In vivo nicking and rejoining of nuclear DNA in ultraviolet-irradiated radiation-resistant and sensitive strains of Dictyostelium discoideum

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