Mutation of a novel virulence-related gene mltD in Vibrio anguillarum enhances lethality in zebra fish

Xu, Zinan; Wang, Ying; Yin, Heng; Chen, Jixiang; Zhang, Xiaohua

doi:10.1016/j.resmic.2010.08.003

Cited by 23 publications

(20 citation statements)

References 29 publications

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“…As in V. vulnificus, only one RTX cluster of a total of three contains a complete set of rtx genes (8,34). The hemolysin genes are located mainly on Chr1, except for vah1, which was located on Chr2 (25,60,86). The metalloprotease genes are located on Chr2 (44,46).…”

Section: Resultsmentioning

confidence: 99%

Complete Genome Sequence of the Marine Fish Pathogen Vibrio anguillarum Harboring the pJM1 Virulence Plasmid and Genomic Comparison with Other Virulent Strains of V. anguillarum and V. ordalii

et al. 2011

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We dissected the complete genome sequence of the O1 serotype strain Vibrio anguillarum 775(pJM1) and determined the draft genomic sequences of plasmidless strains of serotype O1 (strain 96F) and O2␤ (strain RV22) and V. ordalii. All strains harbor two chromosomes, but 775 also harbors the virulence plasmid pJM1, which carries the anguibactin-producing and cognate transport genes, one of the main virulence factors of V. anguillarum. Genomic analysis identified eight genomic islands in chromosome 1 of V. anguillarum 775(pJM1) and two in chromosome 2. Some of them carried potential virulence genes for the biosynthesis of O antigens, hemolysins, and exonucleases as well as others for sugar transport and metabolism. The majority of genes for essential cell functions and pathogenicity are located on chromosome 1. In contrast, chromosome 2 contains a larger fraction (59%) of hypothetical genes than does chromosome 1 (42%). Chromosome 2 also harbors a superintegron, as well as host "addiction" genes that are typically found on plasmids. Unique distinctive properties include homologues of type III secretion system genes in 96F, homologues of V. cholerae zot and ace toxin genes in RV22, and the biofilm formation syp genes in V. ordalii. Mobile genetic elements, some of them possibly originated in the pJM1 plasmid, were very abundant in 775, resulting in the silencing of specific genes, with only few insertions in the 96F and RV22 chromosomes.Vibrio anguillarum is a marine pathogen that causes vibriosis in close to 50 species of fish, including cultured and wild fish, mollusks, and crustaceans, in marine, brackish, and fresh water (1). Vibriosis is a hemorrhagic septicemia with dire consequences for fish rearing, especially in countries that depend heavily on fish for their food consumption. Despite the fact that V. anguillarum is a dramatic cause of vibriosis in fish, little is known about the genomic composition of this important pathogen. Although 23 serotypes have been reported in V. anguillarum, the O1 and O2 serotypes are the major causative agent of fish vibriosis (32,66,75). Many O1 serotype strains harbor 65-kb pJM1-type plasmids, which carry the siderophore anguibactin biosynthesis and transport genes, a main virulence factor of V. anguillarum, while one of the O1 serotype strains and other serotypes, such as all of the O2 strains, are plasmidless (1, 13, 33, 76). Many virulence factors have been characterized, but we are still far from getting the whole picture of the virulence mechanisms of this pathogen (1, 50). The fact that the pJM1 plasmid is an important component of virulence for the 775 strain but that the other three strains examined do not harbor this plasmid and are still virulent indicates that they must have different mechanisms to cause disease. Moreover, O1 serotype strains cause disease in salmonid fish, whereas O2␤ strains are usually isolated from cod and other nonsalmonids (1, 32, 43). We have previously performed random genome sequencing of V. anguillarum 775 genomic DNA and identified potent...

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Section: Resultsmentioning

confidence: 99%

Complete Genome Sequence of the Marine Fish Pathogen Vibrio anguillarum Harboring the pJM1 Virulence Plasmid and Genomic Comparison with Other Virulent Strains of V. anguillarum and V. ordalii

et al. 2011

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“…Immediately flanking the attB site is the his-tRNA, then the parRS operon, encoding the two-component regulatory system ParR-ParS, involved in adaptive resistance to polymyxin B and colistin [35]. A gene encoding a membrane-bound lytic murein transglycosylase (mltD), a virulence-related factor that may participate in beta-lactam resistance is located a few kilobases away [36]. …”

Section: Discussionmentioning

confidence: 99%

Large Preferred Region for Packaging of Bacterial DNA by phiC725A, a Novel Pseudomonas aeruginosa F116-Like Bacteriophage

et al. 2017

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Bacteriophage vB_PaeP_PAO1_phiC725A (short name phiC725A) was isolated following mitomycin C induction of C7-25, a clinical Pseudomonas aeruginosa strain carrying phiC725A as a prophage. The phiC725A genome sequence shows similarity to F116, a P. aeruginosa podovirus capable of generalized transduction. Likewise, phiC725A is a podovirus with long tail fibers. PhiC725A was able to lysogenize two additional P. aeruginosa strains in which it was maintained both as a prophage and in an episomal state. Investigation by deep sequencing showed that bacterial DNA carried inside phage particles originated predominantly from a 700-800kb region, immediately flanking the attL prophage insertion site, whether the phages were induced from a lysogen or recovered after infection. This indicates that during productive replication, recombination of phage genomes with the bacterial chromosome at the att site occurs occasionally, allowing packaging of adjacent bacterial DNA.

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“…Therefore, differences in susceptibility to vibriosis were not completely due to a differential sensitivity of fish to the extracellular products of this bacterium. In L. anguillarum, a membrane-bound lytic murein transglycosylase D (mltD) mutant was generated and its extracellular protease activity decreased markedly together with a total loss of haemolytic activity compared with the wild type strain (Xu et al, 2011). The MltD protein was characterized and it showed haemolytic, phospholipase, gelatinase and diastase activities.…”

Section: Extracellular Products (Ecps)mentioning

confidence: 99%

“…Surprisingly, virulence of the mltD mutant was enhanced compared with that of the wild type when it was inoculated intraperitoneally into zebra fish. This could be partially explained by the hypothesis that peptidoglycan (PG) fragments, released during growth, can contribute to the pathogenesis of multiple bacterial infections (Xu et al, 2011).…”

Section: Extracellular Products (Ecps)mentioning

confidence: 99%

An Overview of Virulence-Associated Factors of Gram-Negative Fish Pathogenic Bacteria

Méndez¹,

Reimundo²,

Pérez-Pascual³

et al. 2012

Health and Environment in Aquaculture

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Mutation of a novel virulence-related gene mltD in Vibrio anguillarum enhances lethality in zebra fish

Cited by 23 publications

References 29 publications

Complete Genome Sequence of the Marine Fish Pathogen Vibrio anguillarum Harboring the pJM1 Virulence Plasmid and Genomic Comparison with Other Virulent Strains of V. anguillarum and V. ordalii

Complete Genome Sequence of the Marine Fish Pathogen Vibrio anguillarum Harboring the pJM1 Virulence Plasmid and Genomic Comparison with Other Virulent Strains of V. anguillarum and V. ordalii

Large Preferred Region for Packaging of Bacterial DNA by phiC725A, a Novel Pseudomonas aeruginosa F116-Like Bacteriophage

An Overview of Virulence-Associated Factors of Gram-Negative Fish Pathogenic Bacteria

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